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The combined bacterial Lux-Fluoro test for the detection and quantification of genotoxic and cytotoxic agents in surface water: Results from the “Technical Workshop on Genotoxicity Biosensing”

Baumstark-Khan, Christa and Rabbow, Elke and Rettberg, Petra and Horneck, Gerda (2007) The combined bacterial Lux-Fluoro test for the detection and quantification of genotoxic and cytotoxic agents in surface water: Results from the “Technical Workshop on Genotoxicity Biosensing”. Aquatic Toxicology, 85 (3), pp. 209-218. Elsevier. doi: 10.1016/j.aquatox.2007.09.003.

Full text not available from this repository.

Abstract

The bioassay Lux-Fluoro testwas developed for the rapid detection and quantification of environmental pollutants with genotoxic and/or cytotoxic potential. This bacterial test system uses two different reporter genes whose gene products and their reactions, respectively, can be measured easily and simultaneously by optical methods. Genotoxicity is measured by the increase of bioluminescence in genetically modified bacteria which carry a plasmid with a complete lux operon for the enzyme luciferase from the marine photobacterium P. leiognathi under the control of a DNA-damage dependent so-called SOS promoter. If the deoxyribonucleic acid in these bacteria is damaged by a genotoxic chemical, the SOS promoter is turned on and the lux operon is expressed. The newly synthesized luciferase reacts immediately with its substrate thereby producing bioluminescence in a damage-proportional manner. In the second part of the system, genetically modified bacteria carry the gene for the green fluorescent protein (gfp) from the jellyfish Aequora victoria downstream from a constitutively expressed promoter. These bacteria are fluorescent under common growth conditions. If their cellular metabolism is disturbed by the action of cytotoxic chemicals, the fluorescence decreases in a dose-proportional manner. The combined Lux-Fluoro test is shown to be well suited for the biological assessment of the geno- and cytotoxicity of a series of model agents and environmental samples at the Technical Workshop on Genotoxicity Biosensing (TECHNOTOX).

Item URL in elib:https://elib.dlr.de/51838/
Document Type:Article
Title:The combined bacterial Lux-Fluoro test for the detection and quantification of genotoxic and cytotoxic agents in surface water: Results from the “Technical Workshop on Genotoxicity Biosensing”
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iD
Baumstark-Khan, ChristaUNSPECIFIEDUNSPECIFIED
Rabbow, ElkeUNSPECIFIEDUNSPECIFIED
Rettberg, PetraUNSPECIFIEDUNSPECIFIED
Horneck, GerdaUNSPECIFIEDUNSPECIFIED
Date:2007
Journal or Publication Title:Aquatic Toxicology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:Yes
Volume:85
DOI :10.1016/j.aquatox.2007.09.003
Page Range:pp. 209-218
Publisher:Elsevier
Status:Published
Keywords:Salmonella typhimurium, Recombinant bacteria, Chemical exposure, Deoxyribonucleic acid damage repair, Bioluminescence, Fluorescence, Green fluorescent protein, Genotoxicity, Cytotoxicity, TECHNOTOX workshop
HGF - Research field:Aeronautics, Space and Transport (old)
HGF - Program:Space (old)
HGF - Program Themes:W FR - Forschung unter Weltraumbedingungen (old)
DLR - Research area:Space
DLR - Program:W FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):W - Vorhaben Strahlenbiologie (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:20 Nov 2007
Last Modified:27 Apr 2009 14:28

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