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Immobilization as a technical possibility for long-term storage of bacterial biosensors

Park, Kyeong Seo and Baumstark-Khan, Christa and Rettberg, Petra and Horneck, Gerda and Rabbow, Elke and Gu, Man Bock (2005) Immobilization as a technical possibility for long-term storage of bacterial biosensors. Radiat Environ Biophys, 44, pp. 69-71. Springer-Verlag.

Full text not available from this repository.

Abstract

For applications in field experiments, the recombinant strain Salmonella typhimurium TA1535 was immobilized to permit its immediate utilization after long storage periods. Salmonella typhimurium TA1535 cells contain the plasmid that has an inducible SOS promoter fused to a promoterless luxCDABFE operon from Photobacterium leiognathi. The induction of bioluminescence occurs in the presence of the DNA-damaging agent mitomycin C which stimulates the bacterial SOS response. Early stationary phase cells were immobilized at a cell concentration of 10<sup>10</sup> CFU/ml in microtiter plates and stored up to 6 weeks at 4°C in a sealed container. Even after 4 weeks of storage, the bioluminescence kinetics and yield in response to different concentrations of mitomycin C were not significantly different from those of freshly prepared samples.

Item URL in elib:https://elib.dlr.de/47585/
Document Type:Article
Title:Immobilization as a technical possibility for long-term storage of bacterial biosensors
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Park, Kyeong SeoNational Research Laboratory on Environmental Biotechnology,Gwangju Institute of Science and Technology (GIST), 1 Oryong-dong, Buk-gu, Gwangju, 500-712, Republic of KoreaUNSPECIFIED
Baumstark-Khan, ChristaUNSPECIFIEDUNSPECIFIED
Rettberg, PetraUNSPECIFIEDUNSPECIFIED
Horneck, GerdaUNSPECIFIEDUNSPECIFIED
Rabbow, ElkeUNSPECIFIEDUNSPECIFIED
Gu, Man BockNational Research Laboratory on Environmental Biotechnology,Gwangju Institute of Science and Technology (GIST), 1 Oryong-dong, Buk-gu, Gwangju, 500-712, Republic of KoreaUNSPECIFIED
Date:2005
Journal or Publication Title:Radiat Environ Biophys
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Volume:44
Page Range:pp. 69-71
Publisher:Springer-Verlag
Status:Published
Keywords:long-term storage of bacterial biosensors, Immobilization, Salmonella typhimurium, DNA-damage
HGF - Research field:Aeronautics, Space and Transport (old)
HGF - Program:Space (old)
HGF - Program Themes:W FR - Forschung unter Weltraumbedingungen (old)
DLR - Research area:Space
DLR - Program:W FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):W - Vorhaben Strahlenbiologie (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:19 Mar 2007
Last Modified:27 Apr 2009 13:35

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