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Determination of Geno- and Cytotoxicity of Groundwater and Sediments Using the Recombinant SWlTCH Test*

Baumstark-Khan, Christa and Cioara, Katharina and Rettberg, Petra and Horneck, Gerda (2005) Determination of Geno- and Cytotoxicity of Groundwater and Sediments Using the Recombinant SWlTCH Test*. Journal of Environmental Science and Health, A 40, pp. 245-263. Taylor & Francis. ISSN 1093-4529 (Print): 1532-4117 (Online)

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Abstract

The recombinant Salmonella typhimurium. TA1535 strain carrying the SWITCH plasmid (combined construct of the SOS-Lux plasmid pPLS-1. and the LAC-Fluoro plasmid pGFPuv: pSWITCH) was treated with control substances for genotoxic (2-aminoanthracene) and cytotoxic (aureomycin) potency as well as with 18 environmental samples (groundwater, river water, sediments) provided at the SENSPOL Technical Meeting on Problems Related to Diffuse Pollution Sources (Characterization of Sediment, Dredged Material, and Groundwater) organized by the Federal Institute of Hydrology in Koblenz, Germany, during late October 2003. For metabolic activation the samples were treated with S9 mix (5% S9 fraction in cofactor mix, Aroclor 1254- induced rat liver microsomes, Moltox Inc., Mol, Belgium), Simultaneously determined cytotoxicity and genotoxicity data were derived through the Multilabel Countear 1420 Victor<sup>2</sup> (ParkinElmer, Boston, MA, USA), by sequential measurement of luminescence, absorbance, and fluorescence. The newly designed SWITCH test, as it was applied at the Koblenz meeting, displays a comparable sensitivity for test samples with known cyto- and genotoxic potential, Groundwater samples from the wells at the former gas plant site Kiel Canal and the agricultural area at Niederwerth expressed neither genotoxic nor cytotoxic responses of the bacteria for both metabolic conditions (+/-S9). Spiked groundwater samples from the Niederwerth well BW1 and the Urmitz well U12 located on the river Rhine were identified to be positive in terms of genotoxicity for the direct and the metabolic approach, samples from the lake Tiefer See in the city of Potsdam showed a ruduction of GFPuv expression as an indication for cytotoxicity, while luminescence output of incubated bacteria remained unaffected. This reflects the well-known presence of contaminations (especially cyanides) in the lake sediment as well as in the acetonic extract. The results obtained at the SENSPOL Technical or the analysis of chemicals under laboratory conditions but also for environmental samples polluted by diffuse industrial sources.

Item URL in elib:https://elib.dlr.de/47580/
Document Type:Article
Title:Determination of Geno- and Cytotoxicity of Groundwater and Sediments Using the Recombinant SWlTCH Test*
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Baumstark-Khan, ChristaUNSPECIFIEDUNSPECIFIED
Cioara, KatharinaUNSPECIFIEDUNSPECIFIED
Rettberg, PetraUNSPECIFIEDUNSPECIFIED
Horneck, GerdaUNSPECIFIEDUNSPECIFIED
Date:2005
Journal or Publication Title:Journal of Environmental Science and Health
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Volume:A 40
Page Range:pp. 245-263
Publisher:Taylor & Francis
ISSN:1093-4529 (Print): 1532-4117 (Online)
Status:Published
Keywords:SOS response, Bioluminescence, Fluorescence, Recombinant bacteria, GFP, Genotoxicity, Cytotoxicity
HGF - Research field:Aeronautics, Space and Transport (old)
HGF - Program:Space (old)
HGF - Program Themes:W FR - Forschung unter Weltraumbedingungen (old)
DLR - Research area:Space
DLR - Program:W FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):W - Vorhaben Strahlenbiologie (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:19 Mar 2007
Last Modified:27 Apr 2009 13:35

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