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DNA damage under simulated extraterrestrial conditions in bacteriophage T7

Fekete, A. and Módos, K. and Hegedüs, M. and Kovács, G. and Rontó, Gy. and Péter, Á and Lammer, H. and Panitz, Corinna (2005) DNA damage under simulated extraterrestrial conditions in bacteriophage T7. Advances in Space Research, 36, pp. 303-310. Elsevier.

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Abstract

The experiment ‘‘Phage and Uracil response’’ will be accommodated in the EXPOSE facility of the International Space Station. Its objective is to examine and quantify the effect of specific space conditions on nucleic acid models, especially on bacteriophage T7 and isolated T7 DNA thin films. In order to define the environmental and technical requirements of the EXPOSE, the samples were subjected to the experiment verification test (EVT). During EVT, the samples were exposed to vacuum (10<sup>-4</sup> – 10<sup>-6</sup> Pa) and polychromatic UV-radiation (200–400 nm) in air, in inert atmosphere, as well as in simulated space vacuum. The effect of extreme temperature in vacuum and the influence of temperature fluctuations around 0°C were also studied. The total intraphage/isolated DNA damage was determined by quantitative PCR using 555 and 3826 bp fragments of T7 DNA. The type of the damage was resolved using a combination of enzymatic probes and neutral and alkaline agarose gel electrophoresis; the structural/chemical effects were analyzed by spectroscopic and microscopical methods. We obtained substantial evidence that DNA lesions accumulate throughout exposure, but the amount of damage depends on the thickness of the layers. According to our preliminary results, the damages by exposure to conditions of dehydration and UV-irradiation are larger than the sum of vacuum alone, or radiation alone case, suggesting a synergistic action of space vacuum and UV radiation with DNA being the critical target.

Item URL in elib:https://elib.dlr.de/47504/
Document Type:Article
Title:DNA damage under simulated extraterrestrial conditions in bacteriophage T7
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iD
Fekete, A.Institute of Biophysics and Radiation Biology, Semmelweis University, H-1088 Budapest, Puskin u. 9, HungaryUNSPECIFIED
Módos, K.Institute of Biophysics and Radiation Biology, Semmelweis University, H-1088 Budapest, Puskin u. 9, HungaryUNSPECIFIED
Hegedüs, M.Institute of Biophysics and Radiation Biology, Semmelweis University, H-1088 Budapest, Puskin u. 9, HungaryUNSPECIFIED
Kovács, G.MTA-SE Research Group for Biophysics, Hungarian Academy of Sciences, H-1088 Budapest, Puskin u. 9, HungaryUNSPECIFIED
Rontó, Gy.MTA-SE Research Group for Biophysics, Hungarian Academy of Sciences, H-1088 Budapest, Puskin u. 9, HungaryUNSPECIFIED
Péter, ÁResearch Institute for Solid State Physics and Optics, Hungarian Academy of Sciences, H-1121 Budapest, Konkoly-Thege u. 29-33, HungaryUNSPECIFIED
Lammer, H.Department for Extraterrestrial Physics, Space Research Institute, Austrian Academy of Sciences, Schmiedstrasse 6, A-8042 Graz, AustriaUNSPECIFIED
Panitz, CorinnaUNSPECIFIEDUNSPECIFIED
Date:2005
Journal or Publication Title:Advances in Space Research
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:Yes
Volume:36
Page Range:pp. 303-310
Editors:
EditorsEmailEditor's ORCID iD
COSPAR, UNSPECIFIEDUNSPECIFIED
Publisher:Elsevier
Status:Published
Keywords:Astrobiology, Bacteriophage, DNA thin film, Phage thin layer, Space vacuum, Solar UV radiation, DNA damage
HGF - Research field:Aeronautics, Space and Transport (old)
HGF - Program:Space (old)
HGF - Program Themes:W FR - Forschung unter Weltraumbedingungen (old)
DLR - Research area:Space
DLR - Program:W FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):W - Vorhaben Strahlenbiologie (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:05 Mar 2007
Last Modified:27 Apr 2009 13:34

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