elib
DLR-Header
DLR-Logo -> http://www.dlr.de
DLR Portal Home | Imprint | Privacy Policy | Contact | Deutsch
Fontsize: [-] Text [+]

Cellular monitoring systems for the assessment of space environmental factors

Hellweg, Christine E. and Arenz, Andrea and Meier, Matthias M. and Baumstark-Khan, Christa (2005) Cellular monitoring systems for the assessment of space environmental factors. Advances in Space Research, 36, pp. 1673-1679. Elsevier.

Full text not available from this repository.

Abstract

Detrimental environmental factors – namely ionizing radiation – will continue to affect future manned space missions. The Cellular Biodiagnostics group at the German Aerospace Center (DLR) develops cellular monitoring systems, which include bacterial and mammalian cell systems capable of responding to DNA damage as a consequence of the presence of genotoxic conditions. Such bioassays will complement the physical detector systems used in space, insofar as they yield intrinsically biologically weighted measures of cellular responses. Furthermore, synergistic toxic impacts of the radiation environment together with other potentially genotoxic constituents of the space habitat can be quantified using such systems. The biological end-point under investigation in this work is the gene activation by radiation in mammalian cells, based on fluorescent promoter reporter systems using the destabilized enhanced green fluorescent protein variant (d2EGFP). The promoter element to be investigated reflects the activity of the nuclear factor κB (NF-κB) pathway. The NF-κB family of proteins plays a major role in the inflammatory and immune response, cell proliferation and differentiation, apoptosis and tumorigenesis. After exposure to X-rays, an increase in NF-κB activation was seen only with high doses. Experiments using accelerated argon ions (95 MeV/u, LET ∼230 keV/μm) produced at the French heavy ion accelerator GANIL have shown activation of the NF-κB pathway with doses greater than 1 x 10<sup>6</sup> particles cm<sup>-2</sup> (P cm<sup>-2</sup>), reaching its maximal activation at 2 x 10<sup>7</sup> P cm<sup>-2</sup>. These results suggest that the exceptional radiation field in space may activate the NF-κB pathway in human cells.

Item URL in elib:https://elib.dlr.de/47429/
Document Type:Article
Title:Cellular monitoring systems for the assessment of space environmental factors
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Hellweg, Christine E.UNSPECIFIEDUNSPECIFIED
Arenz, AndreaUNSPECIFIEDUNSPECIFIED
Meier, Matthias M.UNSPECIFIEDUNSPECIFIED
Baumstark-Khan, ChristaUNSPECIFIEDUNSPECIFIED
Date:2005
Journal or Publication Title:Advances in Space Research
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:Yes
Volume:36
Page Range:pp. 1673-1679
Editors:
EditorsEmail
COSPAR, UNSPECIFIED
Publisher:Elsevier
Status:Published
Keywords:Bioassay, Gene activation, Genotoxicity, Green Fluoroscent Protein, Nuclear Factor kappa B, Radiation, Space
HGF - Research field:Aeronautics, Space and Transport (old)
HGF - Program:Space (old)
HGF - Program Themes:W FR - Forschung unter Weltraumbedingungen (old)
DLR - Research area:Space
DLR - Program:W FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):W - Vorhaben Strahlenbiologie (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:05 Mar 2007
Last Modified:27 Apr 2009 13:33

Repository Staff Only: item control page

Browse
Search
Help & Contact
Information
electronic library is running on EPrints 3.3.12
Copyright © 2008-2017 German Aerospace Center (DLR). All rights reserved.