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Genotoxicity testing on the international space station: Preparatory work on the SOS-LUX test as part of the space experiment TRIPLE-LUX

Stojicic, Nevena and Walrafen, David and Baumstark-Khan, Christa and Rabbow, Elke and Rettberg, Petra and Weisshaar, Maria-Paz and Horneck, Gerda (2005) Genotoxicity testing on the international space station: Preparatory work on the SOS-LUX test as part of the space experiment TRIPLE-LUX. Advances in Space Research, 36, pp. 1710-1717. Elsevier.

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Abstract

Harmful environmental factors – namely ionizing radiation – will continue to influence future manned space missions. The Radiation Biology Unit at the German Aerospace Center (DLR) develops cellular monitoring systems, which include bacterial and mammalian cell systems capable of recognizing DNA damage as a consequence of the presence of genotoxic conditions. Such a bioassay is the SOS-LUX test, which represents the radiobiological part of the German space experiment ‘‘Gene, immune and cellular responses to single and combined space flight conditions (TRIPLE-LUX)’’ which has been selected by the IDI/USRA Peer Review Panel for NASA/ESA to be performed on the International Space Station (ISS). It will supply basic information on the genotoxic response to radiation applied in microgravity. The biological end-point under investigation will depend on the bacterial SOS response brought about by genetically modified bacteria that are transformed with the pSWITCH plasmid (constructed from the plasmids pPLS-1 and pGFPuv). The luminescent/fluorescent bioassay SWITCH (SWITCH: Salmonella Weighting of Induced Toxicity Cyto/GenoTox for Human Health) as successor of the SOS-LUX test for rapid toxicity (genotoxicity and cytotoxicity) testing, makes use of two sensing and reporting systems for the two biological endpoints under investigation: the SOS-LUX test and the LAC-Fluoro test. The SWITCH plasmid carries the promoterless lux operon of Photobacterium leiognathi as reporter element under the control of the DNA-damage-dependent SOS promoter of ColD as sensor element (for genotoxicity testing) and the sequences for a hybrid protein consisting of b-galactosidase and GFPuv of Aequorea victoria as reporter element under the control of the (in Salmonella constitutively active) LAC promoter of Escherichia coli as sensor element (for cytotoxicity testing). The system has worked properly for terrestrial applications during the first experiments. Experiments using X-rays and UV radiation of various qualities (from UVC to UVA) have given insights into cellular mechanisms relevant for estimation of health risks, resulting from exposure of astronauts to the extraordinary radiation environment of space.

Item URL in elib:https://elib.dlr.de/47428/
Document Type:Article
Title:Genotoxicity testing on the international space station: Preparatory work on the SOS-LUX test as part of the space experiment TRIPLE-LUX
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Stojicic, NevenaUNSPECIFIEDUNSPECIFIED
Walrafen, DavidUNSPECIFIEDUNSPECIFIED
Baumstark-Khan, ChristaUNSPECIFIEDUNSPECIFIED
Rabbow, ElkeUNSPECIFIEDUNSPECIFIED
Rettberg, PetraUNSPECIFIEDUNSPECIFIED
Weisshaar, Maria-PazDepartment of Natural Sciences, University of Applied Sciences Bonn-Rhein-Sieg, Rheinbach, GermanyUNSPECIFIED
Horneck, GerdaUNSPECIFIEDUNSPECIFIED
Date:2005
Journal or Publication Title:Advances in Space Research
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:Yes
Volume:36
Page Range:pp. 1710-1717
Editors:
EditorsEmail
COSPAR, UNSPECIFIED
Publisher:Elsevier
Status:Published
Keywords:Space, Bioluminescence, Green fluorescent protein, Radiation, Bioassay, Genotoxicity, SOS-LUX test
HGF - Research field:Aeronautics, Space and Transport (old)
HGF - Program:Space (old)
HGF - Program Themes:W FR - Forschung unter Weltraumbedingungen (old)
DLR - Research area:Space
DLR - Program:W FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):W - Vorhaben Strahlenbiologie (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:05 Mar 2007
Last Modified:27 Apr 2009 13:33

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