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Gene expression in mammalian cells after exposure to 95 MeV/amu argon ions

Arenz, Andrea and Hellweg, Christine E. and Meier, Matthias M. and Baumstark-Khan, Christa (2005) Gene expression in mammalian cells after exposure to 95 MeV/amu argon ions. Advances in Space Research, 36, pp. 1680-1688. Elsevier.

Full text not available from this repository.

Abstract

High LET radiations, such as heavy ions or neutrons, have an increased biological effectiveness compared to X-rays for gene mutation, genomic instability and carcinogenesis. Estimating the biological risks from space radiation encountered by cosmonauts will continue to influence long term duration in space, such as the planned mission to Mars. The human radiation responsive genes CDKN1A (p21/WAF), GADD45a (GADD45), GADD45b (MyD118), RRM2b (p53R2) and BRCA2 (FancD1), involved in cell cycle control or damage repair, were screened for gene expression changes in MCF-7 cells by quantitative real-time reverse transcription PCR (qRT-PCR) assay, using cDNA obtained from total RNA isolated at various time points after irradiation with accelerated doses of 36-argon ions and X-rays. Examination of the expression profiles 2 and 12 h after exposure reveals a pattern consistent with a population of cells in the early response to DNA damage and invoking cell stress responses. Interesting new data showing different expression patterns according to the gene and the type of ionizing radiation used could be obtained. Results show, that the signaling and repair activities induced after heavy ion or X-ray exposure are not the same and gene expression patterns may become useful indicators for distinguishing different types of radiation in relation to their biological effects.

Item URL in elib:https://elib.dlr.de/47425/
Document Type:Article
Title:Gene expression in mammalian cells after exposure to 95 MeV/amu argon ions
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iD
Arenz, AndreaUNSPECIFIEDUNSPECIFIED
Hellweg, Christine E.UNSPECIFIEDUNSPECIFIED
Meier, Matthias M.UNSPECIFIEDUNSPECIFIED
Baumstark-Khan, ChristaUNSPECIFIEDUNSPECIFIED
Date:2005
Journal or Publication Title:Advances in Space Research
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:Yes
Volume:36
Page Range:pp. 1680-1688
Editors:
EditorsEmailEditor's ORCID iD
COSPAR, UNSPECIFIEDUNSPECIFIED
Publisher:Elsevier
Status:Published
Keywords:Space, Human radiation-responsive genes, Gene activation, Quantitative RT-PCR
HGF - Research field:Aeronautics, Space and Transport (old)
HGF - Program:Space (old)
HGF - Program Themes:W FR - Forschung unter Weltraumbedingungen (old)
DLR - Research area:Space
DLR - Program:W FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):W - Vorhaben Strahlenbiologie (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:28 Feb 2007
Last Modified:27 Apr 2009 13:33

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