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Determination of the microbial diversity of spacecraft assembly, testing and launch facilities: First results of the ESA project MiDiv

Rettberg, Petra and Fritze, D. and Verbarg, S. and Nellen, Jörg and Horneck, Gerda and Stackebrandt, E. and Kminek, G. (2006) Determination of the microbial diversity of spacecraft assembly, testing and launch facilities: First results of the ESA project MiDiv. Advances in Space Research, 38, pp. 1260-1265. Elsevier.

Full text not available from this repository.

Abstract

In the near future, an increasing number of in situ life detection and sample return missions to planets and other solar system bodies will be launched. The demand to control spacecraft-carried microbial contamination becomes obvious. COSPAR (Committee of Space Research) has defined guidelines and bioburden limits for different types of missions and target bodies. The first step in the implementation of these planetary protection guidelines encompasses a qualitative and quantitative inventory of the bioburden of spacecraft assembly facilities. With information about the composition of these microbial communities the development and/or optimization of adequate cleaning, disinfection, and sterilization procedures for spacecraft preparation before launch will be possible. In the ESA project MiDiv, we started to investigate the diversity of cultivable microorganisms found on spacecraft and spacecraft assembly halls using the satellites SMART-1 and ROSETTA as test objects. The analyses to date include cultivation of microorganisms by varying pH, temperature, oxygen, and pasteurization. A culture collection of bacterial isolates and a database of 16S RNA gene sequences have been established. The results of our preliminary work, including the numbers of colony forming units, differentiated as aerobes and facultative anaerobes as well as their phylogenetic classification, give a first overview of the breadth of physiological potential of the identified microorganisms and their capability to withstand various cleaning and sterilizing procedures currently used for the planetary protection.

Item URL in elib:https://elib.dlr.de/47285/
Document Type:Article
Title:Determination of the microbial diversity of spacecraft assembly, testing and launch facilities: First results of the ESA project MiDiv
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Rettberg, PetraUNSPECIFIEDUNSPECIFIED
Fritze, D.German Collection of Microorganisms and Cell Cultures (DSMZ), Mascheroder Weg 1b, D-38124 Braunschweig, GermanyUNSPECIFIED
Verbarg, S.German Collection of Microorganisms and Cell Cultures (DSMZ), Mascheroder Weg 1b, D-38124 Braunschweig, GermanyUNSPECIFIED
Nellen, JörgUNSPECIFIEDUNSPECIFIED
Horneck, GerdaUNSPECIFIEDUNSPECIFIED
Stackebrandt, E.German Collection of Microorganisms and Cell Cultures (DSMZ), Mascheroder Weg 1b, D-38124 Braunschweig, GermanyUNSPECIFIED
Kminek, G.European Space Agency, D/HME, ESTEC, Keplerlaan 1, NL-2200 AG Noordwijk, The NetherlandsUNSPECIFIED
Date:2006
Journal or Publication Title:Advances in Space Research
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:Yes
Volume:38
Page Range:pp. 1260-1265
Editors:
EditorsEmail
COSPAR, UNSPECIFIED
Publisher:Elsevier
Status:Published
Keywords:Planetary protection, Microbial diversity, Clean room, Spacecraft assembly facility, Cultivable microorganisms
HGF - Research field:Aeronautics, Space and Transport (old)
HGF - Program:Space (old)
HGF - Program Themes:W FR - Forschung unter Weltraumbedingungen (old)
DLR - Research area:Space
DLR - Program:W FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):W - Vorhaben Strahlenbiologie (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:28 Feb 2007
Last Modified:27 Apr 2009 13:31

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