Rettberg, Petra and Fritze, D. and Verbarg, S. and Nellen, Jörg and Horneck, Gerda and Stackebrandt, E. and Kminek, G. (2006) Determination of the microbial diversity of spacecraft assembly, testing and launch facilities: First results of the ESA project MiDiv. Advances in Space Research, 38, pp. 1260-1265. Elsevier.
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Abstract
In the near future, an increasing number of in situ life detection and sample return missions to planets and other solar system bodies will be launched. The demand to control spacecraft-carried microbial contamination becomes obvious. COSPAR (Committee of Space Research) has defined guidelines and bioburden limits for different types of missions and target bodies. The first step in the implementation of these planetary protection guidelines encompasses a qualitative and quantitative inventory of the bioburden of spacecraft assembly facilities. With information about the composition of these microbial communities the development and/or optimization of adequate cleaning, disinfection, and sterilization procedures for spacecraft preparation before launch will be possible. In the ESA project MiDiv, we started to investigate the diversity of cultivable microorganisms found on spacecraft and spacecraft assembly halls using the satellites SMART-1 and ROSETTA as test objects. The analyses to date include cultivation of microorganisms by varying pH, temperature, oxygen, and pasteurization. A culture collection of bacterial isolates and a database of 16S RNA gene sequences have been established. The results of our preliminary work, including the numbers of colony forming units, differentiated as aerobes and facultative anaerobes as well as their phylogenetic classification, give a first overview of the breadth of physiological potential of the identified microorganisms and their capability to withstand various cleaning and sterilizing procedures currently used for the planetary protection.
Item URL in elib: | https://elib.dlr.de/47285/ | ||||||||||||||||||||||||||||||||
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Document Type: | Article | ||||||||||||||||||||||||||||||||
Title: | Determination of the microbial diversity of spacecraft assembly, testing and launch facilities: First results of the ESA project MiDiv | ||||||||||||||||||||||||||||||||
Authors: |
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Date: | 2006 | ||||||||||||||||||||||||||||||||
Journal or Publication Title: | Advances in Space Research | ||||||||||||||||||||||||||||||||
Refereed publication: | Yes | ||||||||||||||||||||||||||||||||
Open Access: | No | ||||||||||||||||||||||||||||||||
Gold Open Access: | No | ||||||||||||||||||||||||||||||||
In SCOPUS: | No | ||||||||||||||||||||||||||||||||
In ISI Web of Science: | Yes | ||||||||||||||||||||||||||||||||
Volume: | 38 | ||||||||||||||||||||||||||||||||
Page Range: | pp. 1260-1265 | ||||||||||||||||||||||||||||||||
Editors: |
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Publisher: | Elsevier | ||||||||||||||||||||||||||||||||
Status: | Published | ||||||||||||||||||||||||||||||||
Keywords: | Planetary protection, Microbial diversity, Clean room, Spacecraft assembly facility, Cultivable microorganisms | ||||||||||||||||||||||||||||||||
HGF - Research field: | Aeronautics, Space and Transport (old) | ||||||||||||||||||||||||||||||||
HGF - Program: | Space (old) | ||||||||||||||||||||||||||||||||
HGF - Program Themes: | W FR - Forschung unter Weltraumbedingungen (old) | ||||||||||||||||||||||||||||||||
DLR - Research area: | Space | ||||||||||||||||||||||||||||||||
DLR - Program: | W FR - Forschung unter Weltraumbedingungen | ||||||||||||||||||||||||||||||||
DLR - Research theme (Project): | W - Vorhaben Strahlenbiologie (old) | ||||||||||||||||||||||||||||||||
Location: | Köln-Porz | ||||||||||||||||||||||||||||||||
Institutes and Institutions: | Institute of Aerospace Medicine > Radiation Biology | ||||||||||||||||||||||||||||||||
Deposited By: | Kopp, Kerstin | ||||||||||||||||||||||||||||||||
Deposited On: | 28 Feb 2007 | ||||||||||||||||||||||||||||||||
Last Modified: | 27 Apr 2009 13:31 |
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