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The SOS-LUX-TOXICITY-Test on the International Space Station

Rabbow, Elke and Stojicic, Nevena and Walrafen, David and Baumstark-Khan, Christa and Rettberg, Petra and Schulze-Varnholt, D. and Franz, Markus and Reitz, Günther (2006) The SOS-LUX-TOXICITY-Test on the International Space Station. Research in Microbiology, 157, pp. 30-36. Elsevier.

Full text not available from this repository.

Abstract

For the safety of astronauts and to ensure the stability and integrity of the genome of microorganisms and plants used in bioregenerative life support systems, it is important to improve our knowledge of the combined action of (space) radiation and microgravity. The SOS-LUXTOXICITY test, as part of the TRIPLE-LUX project (accepted for flight at Biolab in Columbus on the International Space Station, (ISS)), will provide an estimation of the health risk resulting from exposure of astronauts to the radiation environment of space in microgravity. The project will: (i) increase our knowledge of biological/health threatening action of space radiation and enzymatic DNA repair; (ii) uncover cellular mechanisms of synergistic interaction of microgravity and space radiation; (iii) provide specified biosensors for spacecraft milieu examination; and (iv) provide experimental data on stability and integrity of bacterial DNA in spacecrafts. In the bacterial biosensor “SOS-LUX-Test” developed at DLR (patent), bacteria are transformed with the pBR322-derived plasmid pPLS-1 or the similar, advanced plasmid SWITCH, both carrying the promoterless lux operon of Photobacterium leiognathi as the reporter element controlled by a DNA damage-dependent SOS promoter as sensor element. A short description of the space experiment is given, and the current status of adaptation of the SOS-LUX-Test to the ISS, i.e. first results of sterilization, biocompatibility and functional tests performed with the already available hardware and bread board model of the automated space hardware under development, is described here.

Item URL in elib:https://elib.dlr.de/47248/
Document Type:Article
Title:The SOS-LUX-TOXICITY-Test on the International Space Station
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Rabbow, ElkeUNSPECIFIEDUNSPECIFIED
Stojicic, NevenaUNSPECIFIEDUNSPECIFIED
Walrafen, DavidUNSPECIFIEDUNSPECIFIED
Baumstark-Khan, ChristaUNSPECIFIEDUNSPECIFIED
Rettberg, PetraUNSPECIFIEDUNSPECIFIED
Schulze-Varnholt, D.EADS SPACE Transportation GmbH, 88039 Friedrichshafen, GermanyUNSPECIFIED
Franz, MarkusUNSPECIFIEDUNSPECIFIED
Reitz, GüntherUNSPECIFIEDUNSPECIFIED
Date:2006
Journal or Publication Title:Research in Microbiology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:Yes
Volume:157
Page Range:pp. 30-36
Publisher:Elsevier
Status:Published
Keywords:Space, Bioluminescence, Green fluorescent protein, Radiation, Bioassay, Genotoxicity
HGF - Research field:Aeronautics, Space and Transport (old)
HGF - Program:Space (old)
HGF - Program Themes:W FR - Forschung unter Weltraumbedingungen (old)
DLR - Research area:Space
DLR - Program:W FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):W - Vorhaben Strahlenbiologie (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:28 Feb 2007
Last Modified:27 Apr 2009 13:30

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