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Simulated microgravity upregulates gene expression of the skeletal regulator core binding factor α1/Runx2 in Medaka fish larvae in vivo

Renn, Joerg and Seibt, Dieter and Goerlich, Roland and Schartl, Manfred and Winkler, Christian (2006) Simulated microgravity upregulates gene expression of the skeletal regulator core binding factor α1/Runx2 in Medaka fish larvae in vivo. Advances in Space Research, 38, pp. 1025-1031. Elsevier.

Full text not available from this repository.

Abstract

Long-term space flight results in significant bone loss in humans. However, it remains to be shown how microgravity affects the expression of genes involved in modeling and remodeling of bone material in vivo. For these analyses, animal models are instrumental to study alterations at the molecular and cellular level. Although it is not known at present, whether fish lose bone in microgravity, they show many experimental advantages to approach these questions in vivo. Here, we report for the first time that living Medaka larvae can be used in hypergravitation and clinorotation experiments to study the effect of altered gravity on gene expression in a whole-animal situation. Living Medaka larvae at 1 day post-hatching were exposed to hypergravity and simulated microgravity for 24 hours (h) and the level of mRNA expression of skeletal regulators was determined by real-time RT-PCR. No effect of altered gravity was observed on the expression of osteoprotegerin (opg) genes that regulate osteoclast formation in humans. However, clinorotation resulted in a significant increase of expression of core binding factor α1(cbfa1/runx2), a crucial regulator of osteoblast formation. Exposure to hypergravitation for 24 h on the other hand had no effect on cbfa1/runx2 expression. This shows that cbfa1/runx2 responds to reduced gravity by expression level changes in vivo. Furthermore, it demonstrates that Medaka provides a valuable experiental model to study molecular mechanisms for compensating microgravity induced bone loss.

Item URL in elib:https://elib.dlr.de/45968/
Document Type:Article
Title:Simulated microgravity upregulates gene expression of the skeletal regulator core binding factor α1/Runx2 in Medaka fish larvae in vivo
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Renn, JoergUniversity of Wuerzburg and RWTH AachenUNSPECIFIED
Seibt, DieterUNSPECIFIEDUNSPECIFIED
Goerlich, RolandRWTH AachenUNSPECIFIED
Schartl, ManfredUniversity of WuerzburgUNSPECIFIED
Winkler, ChristianUniversity of WuerzburgUNSPECIFIED
Date:2006
Journal or Publication Title:Advances in Space Research
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:Yes
Volume:38
Page Range:pp. 1025-1031
Publisher:Elsevier
Status:Published
Keywords:Animal models, Medaka, cbfa1/runx2, Osteoprotegerin, Clinorotation, Cartilage, Skeleton, Bone loss
HGF - Research field:Aeronautics, Space and Transport (old)
HGF - Program:Space (old)
HGF - Program Themes:W FR - Forschung unter Weltraumbedingungen (old)
DLR - Research area:Space
DLR - Program:W FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):W - Vorhaben Biowissenschaftliche Nutzerunterstützung (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Biomedical Science Support Center
Deposited By: Lütz, Gertrud
Deposited On:20 Feb 2007
Last Modified:27 Apr 2009 13:17

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