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Distribution of Membrane Bound Guanylyl Cyclases in Human Intestine

Krause, G. (1) and Bayerl, A. (1) and Heim, J.-M. (1) and Singh, S. (1) and Gerzer, R. (1994) Distribution of Membrane Bound Guanylyl Cyclases in Human Intestine. Gut 1994, 35, pp. 1250-1257.

Full text not available from this repository.

Abstract

The quantification and distinction of particulate guanylyl cyclases in the human intestine were considered by an enzymatic approach, which comprised the signal transduction from receptor binding to cGMP formation, and, in addition, by showing the expression of an intracellular portion of these transmembrane proteins. Basal guany- lyl cyclase (GC) activities were 50 to 80 pmol cGMP formation/min/mg protein and were stimulated up to twofold by heat stable enerotoxin, but were not significantly influenced by atrial natriuretic factor. Enzymatic analysis of colonoscopic specimens pointed to the prevalen-ce of guanylyl cyclase C in the terminal ileum and in the large bowelincluding colon ascendens, colon descendens, sigmoid, and rectum. Theavailability of sequence information on human guanylyl cyclases per- mitted the development of a polymerase chain reaction approach for distinguishing the expression of GC-A and GC-C in human tissue samples. The expression levels of particulate guanylyl cyclases found by polymerase chain reaction in surgical biopsy specimens con- firmed the enzymatic data, in that substantial expression of GC-C was found not only in the small intestine but also in the large bowel. According to the restriction mapping of amplificates, GC-C pre- vailed over GC-A throughout the human intestine, particularly in the mucosal layers.

Item URL in elib:https://elib.dlr.de/27108/
Document Type:Article
Additional Information: LIDO-Berichtsjahr=1994, pages=8,
Title:Distribution of Membrane Bound Guanylyl Cyclases in Human Intestine
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Krause, G. (1)UNSPECIFIEDUNSPECIFIED
Bayerl, A. (1)UNSPECIFIEDUNSPECIFIED
Heim, J.-M. (1)UNSPECIFIEDUNSPECIFIED
Singh, S. (1)UNSPECIFIEDUNSPECIFIED
Gerzer, R.UNSPECIFIEDUNSPECIFIED
Date:1994
Journal or Publication Title:Gut 1994
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:Yes
Volume:35
Page Range:pp. 1250-1257
Status:Published
HGF - Research field:UNSPECIFIED
HGF - Program:Space (old)
HGF - Program Themes:W EO - Erdbeobachtung
DLR - Research area:UNSPECIFIED
DLR - Program:W EO - Erdbeobachtung
DLR - Research theme (Project):UNSPECIFIED
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine
Deposited By: DLR-Beauftragter, elib
Deposited On:02 Apr 2006
Last Modified:27 Apr 2009 06:29

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