Ivanova, Krassimira and Schaefer, M. (1) and Drummer, C. and Gerzer, R. (1993) Effects of NitricOxide-Containing Compounds on Increases in Cytosolic Ionized Ca2+ and on Aggregation of Human Platelets. European Journal of Pharmacology - Molecular Pharmacology Section, 244, pp. 37-47. Elsevier Science Publishers B.V..
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Abstract
The present study was undertaken to determine the modulatory effects of nitric oxide (NO)-releasing compounds on increases in cytosolic ionized calcium ((Ca2+)i) and on aggregation of gel-filtered and human platelets induced via diverse agonists. We used various sydnonimines and organic nitrates as donors of NO. Gel-filtered and fura-2-loaded platelets were stimulated with ADP, collagen, or thrombin, respectively. Half-maximal inhibiting effects of sydno- nimines on agonist-evoked increases in (Ca2+)i were observed between 30 and 1000 nM, while half-maximal inhibiting effects of the compounds on aggregation were between 3 and 500 nM. The compound C 87-3754, which is the bioactive metabolite of pirsidomine, was a much stronger inhibitor of increases in (Ca2+)i than of platelet aggregation. This was due to an enhanced NO release from this compound exposed to ultraviolet light during Ca2+ measurement. The organic nitrates isosorbide 5-mononitrate and nicorandil inhibited both aggregation and increase of cytosolic ionized calcium in stimulated platelets at half-maximal concentrations of approximately 200 nM. The present results also suggest that some of the inhibitory effects of NO-releasing compounds correspond rather to the presence of the A forms (NO-containing intermediates) than to the presence of free NO.
Item URL in elib: | https://elib.dlr.de/26869/ | |||||||||||||||
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Document Type: | Article | |||||||||||||||
Additional Information: | LIDO-Berichtsjahr=1993, pages=11, | |||||||||||||||
Title: | Effects of NitricOxide-Containing Compounds on Increases in Cytosolic Ionized Ca2+ and on Aggregation of Human Platelets | |||||||||||||||
Authors: |
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Date: | 1993 | |||||||||||||||
Journal or Publication Title: | European Journal of Pharmacology - Molecular Pharmacology Section | |||||||||||||||
Refereed publication: | Yes | |||||||||||||||
Open Access: | Yes | |||||||||||||||
Gold Open Access: | Yes | |||||||||||||||
In SCOPUS: | No | |||||||||||||||
In ISI Web of Science: | Yes | |||||||||||||||
Volume: | 244 | |||||||||||||||
Page Range: | pp. 37-47 | |||||||||||||||
Publisher: | Elsevier Science Publishers B.V. | |||||||||||||||
Status: | Published | |||||||||||||||
Keywords: | Nitric oxide (NO), Ca2+, platelet aggregation | |||||||||||||||
HGF - Research field: | Aeronautics, Space and Transport (old) | |||||||||||||||
HGF - Program: | Space (old) | |||||||||||||||
HGF - Program Themes: | W - no assignment | |||||||||||||||
DLR - Research area: | Space | |||||||||||||||
DLR - Program: | W - no assignment | |||||||||||||||
DLR - Research theme (Project): | W - no assignment (old) | |||||||||||||||
Location: | Köln-Porz | |||||||||||||||
Institutes and Institutions: | Institute of Aerospace Medicine | |||||||||||||||
Deposited By: | DLR-Beauftragter, elib | |||||||||||||||
Deposited On: | 02 Apr 2006 | |||||||||||||||
Last Modified: | 11 Jul 2011 13:36 |
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