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Effects of NitricOxide-Containing Compounds on Increases in Cytosolic Ionized Ca2+ and on Aggregation of Human Platelets

Ivanova, Krassimira and Schaefer, M. (1) and Drummer, C. and Gerzer, R. (1993) Effects of NitricOxide-Containing Compounds on Increases in Cytosolic Ionized Ca2+ and on Aggregation of Human Platelets. European Journal of Pharmacology - Molecular Pharmacology Section, 244, pp. 37-47. Elsevier Science Publishers B.V..

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Abstract

The present study was undertaken to determine the modulatory effects of nitric oxide (NO)-releasing compounds on increases in cytosolic ionized calcium ((Ca2+)i) and on aggregation of gel-filtered and human platelets induced via diverse agonists. We used various sydnonimines and organic nitrates as donors of NO. Gel-filtered and fura-2-loaded platelets were stimulated with ADP, collagen, or thrombin, respectively. Half-maximal inhibiting effects of sydno- nimines on agonist-evoked increases in (Ca2+)i were observed between 30 and 1000 nM, while half-maximal inhibiting effects of the compounds on aggregation were between 3 and 500 nM. The compound C 87-3754, which is the bioactive metabolite of pirsidomine, was a much stronger inhibitor of increases in (Ca2+)i than of platelet aggregation. This was due to an enhanced NO release from this compound exposed to ultraviolet light during Ca2+ measurement. The organic nitrates isosorbide 5-mononitrate and nicorandil inhibited both aggregation and increase of cytosolic ionized calcium in stimulated platelets at half-maximal concentrations of approximately 200 nM. The present results also suggest that some of the inhibitory effects of NO-releasing compounds correspond rather to the presence of the A forms (NO-containing intermediates) than to the presence of free NO.

Item URL in elib:https://elib.dlr.de/26869/
Document Type:Article
Additional Information: LIDO-Berichtsjahr=1993, pages=11,
Title:Effects of NitricOxide-Containing Compounds on Increases in Cytosolic Ionized Ca2+ and on Aggregation of Human Platelets
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Ivanova, KrassimiraUNSPECIFIEDUNSPECIFIED
Schaefer, M. (1)UNSPECIFIEDUNSPECIFIED
Drummer, C.UNSPECIFIEDUNSPECIFIED
Gerzer, R.UNSPECIFIEDUNSPECIFIED
Date:1993
Journal or Publication Title:European Journal of Pharmacology - Molecular Pharmacology Section
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:No
In ISI Web of Science:Yes
Volume:244
Page Range:pp. 37-47
Publisher:Elsevier Science Publishers B.V.
Status:Published
Keywords:Nitric oxide (NO), Ca2+, platelet aggregation
HGF - Research field:Aeronautics, Space and Transport (old)
HGF - Program:Space (old)
HGF - Program Themes:W - no assignment
DLR - Research area:Space
DLR - Program:W - no assignment
DLR - Research theme (Project):W - no assignment (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine
Deposited By: DLR-Beauftragter, elib
Deposited On:02 Apr 2006
Last Modified:11 Jul 2011 13:36

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