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A biofilm used as ultraviolet dosimeter

Quintern, L. E. and Horneck, G. and Eschweiler, U. and Bücker, H. (1991) A biofilm used as ultraviolet dosimeter. Photochemistry and Photobiology, Vol. 55 (No. 3), pp. 389-395.

Full text not available from this repository.

Abstract

An UV-dosimeter has been developed for routine measurements which mainly weights the various components of the spectrum in relation to their damaging effects on a microorganism. For this purpose a biofilm was constructed, comprising dried spores of Bacillus subtilis (wild-type or DNA repair defective strain), immobilized on transparent polyester plastic sheets. After irradiation, the biofilm was incubated in a growth medium. The proteins, synthesized by immobilized microorganisms after spore germination and several cell divisions, were stained and determined by photometry, giving a measure of the biological activity. The "biological effective dose" was determined from a calibration curve. It reflects the dose equivalent to that of the calibration source producing the same effect. The UV-response of the biofilm is additive and follows the reciprocity law in the range of fluence rates investigated. The response is nearly independent of temperature within the range of 20 °C to +70 °C as well as of humidity between about 37 % and 80 % relative humidity. Storage for up to 9 months at < 70 % relative humidity and room temperature does not influence the viability of the spores to a significant rate.

Item URL in elib:https://elib.dlr.de/26742/
Document Type:Article
Additional Information: LIDO-Berichtsjahr=1992,
Title:A biofilm used as ultraviolet dosimeter
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Quintern, L. E.UNSPECIFIEDUNSPECIFIED
Horneck, G.UNSPECIFIEDUNSPECIFIED
Eschweiler, U.UNSPECIFIEDUNSPECIFIED
Bücker, H.UNSPECIFIEDUNSPECIFIED
Date:1991
Journal or Publication Title:Photochemistry and Photobiology
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:Yes
Volume:Vol. 55
Page Range:pp. 389-395
Status:Published
Keywords:biological UV-dosimetry, detector film, Bacillus subtilis
HGF - Research field:Aeronautics, Space and Transport (old)
HGF - Program:other
HGF - Program Themes:other
DLR - Research area:Space
DLR - Program:no assignment
DLR - Research theme (Project):UNSPECIFIED
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine
Deposited By: DLR-Beauftragter, elib
Deposited On:02 Apr 2006
Last Modified:27 Apr 2009 06:19

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