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Clinorotation inhibits myotube formation by fluid motion, not by simulated microgravity

Mansour Jamaleddine, Janet and Berwanger, Carolin and Jung, Marcel and Eichinger, Ludwig and Fabry, Ben and Clemen, Christoph S. (2023) Clinorotation inhibits myotube formation by fluid motion, not by simulated microgravity. European Journal of Cell Biology, 102 (2), p. 151330. Elsevier. doi: 10.1016/j.ejcb.2023.151330. ISSN 0171-9335.

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Official URL: https://doi.org/10.1016/j.ejcb.2023.151330

Abstract

To study processes related to weightlessness in ground-based cell biological research, a theoretically assumed microgravity environment is typically simulated using a clinostat - a small laboratory device that rotates cell culture vessels with the aim of averaging out the vector of gravitational forces. Here, we report that the rotational movement during fast clinorotation induces complex fluid motions in the cell culture vessel, which can trigger unintended cellular responses. Specifically, we demonstrate that suppression of myotube formation by 2D-clinorotation at 60 rpm is not an effect of the assumed microgravity but instead is a consequence of fluid motion. Therefore, cell biological results from fast clinorotation cannot be attributed to microgravity unless alternative explanations have been rigorously tested and ruled out. We consider two control experiments mandatory, i) a static, non-rotating control, and ii) a control for fluid motion. These control experiments are also highly recommended for other rotation speed settings and experimental conditions. Finally, we discuss strategies to minimize fluid motion in clinorotation experiments.

Item URL in elib:https://elib.dlr.de/198914/
Document Type:Article
Title:Clinorotation inhibits myotube formation by fluid motion, not by simulated microgravity
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iDORCID Put Code
Mansour Jamaleddine, JanetGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, GermanyUNSPECIFIEDUNSPECIFIED
Berwanger, CarolinGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, GermanyUNSPECIFIEDUNSPECIFIED
Jung, MarcelUniversity of Duisburg-Essen, University Hospital Essen, Institute for Experimental Immunology and Imaging, Essen, GermanyUNSPECIFIEDUNSPECIFIED
Eichinger, LudwigUniversity of Cologne, Medical Faculty, Institute of Biochemistry, Cologne, Germanyhttps://orcid.org/0000-0003-1594-6117UNSPECIFIED
Fabry, BenFriedrich-Alexander University Erlangen-Nürnberg (FAU), Department of Physics, Biophysics Group, 91054 Erlangen, GermanyUNSPECIFIEDUNSPECIFIED
Clemen, Christoph S.German Aerospace Center (DLR), Institute of Aerospace Medicine, Gravitational Biology, Colognehttps://orcid.org/0000-0002-1291-4219UNSPECIFIED
Date:5 June 2023
Journal or Publication Title:European Journal of Cell Biology
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:102
DOI:10.1016/j.ejcb.2023.151330
Page Range:p. 151330
Publisher:Elsevier
ISSN:0171-9335
Status:Published
Keywords:Simulated microgravity, Clinostat, Gravity, Fluid flow, Fluid motion, Myoblasts, Myotubes, Mammalian cell culture, Shear stress
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - Gravisensorics
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Gravitational Biology
Deposited By: Chiodo, Annette
Deposited On:15 Nov 2023 12:11
Last Modified:30 Jan 2024 10:55

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