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Human gut microbiome and metabolite dynamics under simulated microgravity

Ramos-Nascimento, Ana and Grenga, Lucia and Haange, Sven-Bastiaan and Himmelmann, Alexandra and Arndt, Franca Sabine and Ly, Yen-Tran and Miotello, Guylaine and Pible, Olivier and Jehmlich, Nico and Engelmann, Beatrice and von Bergen, Martin and Mulder, Edwin and Frings-Meuthen, Petra and Hellweg, Christine Elisabeth and Jordan, Jens and Rolle-Kampczyk, Ulrike and Armengaud, Jean and Moeller, Ralf (2023) Human gut microbiome and metabolite dynamics under simulated microgravity. Gut Microbes, 15 (2), p. 2259033. Taylor & Francis. doi: 10.1080/19490976.2023.2259033. ISSN 1949-0976.

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Official URL: https://dx.doi.org/10.1080/19490976.2023.2259033

Abstract

The Artificial Gravity Bed Rest – European Space Agency (AGBRESA) study was the first joint bed rest study by ESA, DLR, and NASA that examined the effect of simulated weightlessness on the human body and assessed the potential benefits of artificial gravity as a countermeasure in an analog of long-duration spaceflight. In this study, we investigated the impact of simulated microgravity on the gut microbiome of 12 participants during a 60-day head-down tilt bed rest at the :envihab facilities. Over 60 days of simulated microgravity resulted in a mild change in the gut microbiome, with distinct microbial patterns and pathway expression in the feces of the countermeasure group compared to the microgravity simulation-only group. Additionally, we found that the countermeasure protocols selectively increased the abundance of beneficial short-chain fatty acids in the gut, such as acetate, butyrate, and propionate. Some physiological signatures also included the modulation of taxa reported to be either beneficial or opportunistic, indicating a mild adaptation in the microbiome network balance. Our results suggest that monitoring the gut microbial catalog along with pathway clustering and metabolite profiling is an informative synergistic strategy to determine health disturbances and the outcome of countermeasure protocols for future space missions. Plain Language Summary The future of spaceflight will involve missions beyond the International Space Station or the Moon and astronaut’s health will be challenged by a harsh space environment for longer periods. In the last decade, the intestine has gained importance in dictating overall physiology and we explore it as an additional indicator of health during our ground-based bed rest study simulating microgravity for 60 days. Through the analysis of fecal proteins, we compile the catalog of microbes colonizing the gut of the 12 participants along with the implicated biological activity of the proteins and another 9 lipid analytes. We found specific microbes associated with recovery or healthy status in our subjects to be increased during spaceflight countermeasure conditions and inverse observations in subjects subjected to perilous spaceflight simulation. Our approach improves the functional characterization of the gut by the use of noninvasive methodology correlating the microbial composition of human stool samples with physiological status.

Item URL in elib:https://elib.dlr.de/197592/
Document Type:Article
Title:Human gut microbiome and metabolite dynamics under simulated microgravity
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iDORCID Put Code
Ramos-Nascimento, AnaUNSPECIFIEDhttps://orcid.org/0000-0003-2437-3445UNSPECIFIED
Grenga, LuciaUniversité Paris-Saclay, CEA, INRAE, Département Médicaments et Technologies pour la Santé (DMTS), SPI, Bagnols sur Cèze, Francehttps://orcid.org/0000-0001-5560-1717UNSPECIFIED
Haange, Sven-BastiaanDepartment of Metabolomics, UFZ-Helmholtz Centre for Environmental Research Leipzig, Leipzig, Germanyhttps://orcid.org/0000-0003-2952-1152UNSPECIFIED
Himmelmann, AlexandraUNSPECIFIEDhttps://orcid.org/0009-0003-9478-1185UNSPECIFIED
Arndt, Franca SabineUNSPECIFIEDhttps://orcid.org/0000-0003-0977-3969143094046
Ly, Yen-TranUNSPECIFIEDhttps://orcid.org/0009-0007-0048-0218143094049
Miotello, GuylaineUniversité Paris-Saclay, CEA, INRAE, Département Médicaments et Technologies pour la Santé (DMTS), SPI, Bagnols sur Cèze, Francehttps://orcid.org/0000-0002-2398-1249UNSPECIFIED
Pible, OlivierUniversité Paris-Saclay, CEA, INRAE, Département Médicaments et Technologies pour la Santé (DMTS), SPI, Bagnols sur Cèze, Francehttps://orcid.org/0000-0003-4398-6730UNSPECIFIED
Jehmlich, NicoDepartment of Metabolomics, UFZ-Helmholtz Centre for Environmental Research Leipzig, Leipzig, Germanyhttps://orcid.org/0000-0002-5638-6868UNSPECIFIED
Engelmann, BeatriceDepartment of Metabolomics, UFZ-Helmholtz Centre for Environmental Research Leipzig, Leipzig, Germanyhttps://orcid.org/0000-0002-8807-9651UNSPECIFIED
von Bergen, MartinDepartment of Metabolomics, UFZ-Helmholtz Centre for Environmental Research Leipzig, Leipzig, Germanyhttps://orcid.org/0000-0003-2732-2977UNSPECIFIED
Mulder, EdwinUNSPECIFIEDhttps://orcid.org/0000-0003-1200-5792UNSPECIFIED
Frings-Meuthen, PetraUNSPECIFIEDhttps://orcid.org/0000-0001-5291-4419UNSPECIFIED
Hellweg, Christine ElisabethUNSPECIFIEDhttps://orcid.org/0000-0002-2223-3580UNSPECIFIED
Jordan, JensUNSPECIFIEDhttps://orcid.org/0000-0003-4518-0706UNSPECIFIED
Rolle-Kampczyk, UlrikeDepartment of Metabolomics, UFZ-Helmholtz Centre for Environmental Research Leipzig, Leipzig, Germanyhttps://orcid.org/0000-0002-7728-6284UNSPECIFIED
Armengaud, JeanUniversité Paris-Saclay, CEA, INRAE, Département Médicaments et Technologies pour la Santé (DMTS), SPI, Bagnols sur Cèze, Francehttps://orcid.org/0000-0003-1589-445XUNSPECIFIED
Moeller, RalfUNSPECIFIEDhttps://orcid.org/0000-0002-2371-0676UNSPECIFIED
Date:25 September 2023
Journal or Publication Title:Gut Microbes
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:15
DOI:10.1080/19490976.2023.2259033
Page Range:p. 2259033
Publisher:Taylor & Francis
ISSN:1949-0976
Status:Published
Keywords:Gut microbiome, gut metabolites, functional analysis, metaproteomics, SCFA, microgravity, spaceflight, AGBRESA, bed rest study
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - Project ISS LIFE 2.0
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Institute of Aerospace Medicine > Leitungsbereich ME
Deposited By: Kopp, Kerstin
Deposited On:27 Sep 2023 10:38
Last Modified:27 Sep 2023 10:38

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