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Structural mechanism of extranucleosomal DNA readout by the INO80 complex

Kunert, Franziska and Metzner, Felix J. and Jung, James and Höpfler, Markus and Woike, Stephan and Schall, Kevin and Kostrewa, Dirk and Moldt, Manuela and Chen, Jia-Xuan and Bantele, Susanne and Pfander, Boris and Eustermann, Sebastian and Hopfner, Karl-Peter (2022) Structural mechanism of extranucleosomal DNA readout by the INO80 complex. Science Advances, 8 (49), eadd3189. American Association for the Advancement of Science (AAAS). doi: 10.1126/sciadv.add3189. ISSN 2375-2548.

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Official URL: https://dx.doi.org/10.1126/sciadv.add3189

Abstract

The nucleosomal landscape of chromatin depends on the concerted action of chromatin remodelers. The INO80 remodeler specifically places nucleosomes at the boundary of gene regulatory elements, which is proposed to be the result of an ATP-dependent nucleosome sliding activity that is regulated by extranucleosomal DNA features. Here, we use cryo–electron microscopy and functional assays to reveal how INO80 binds and is regulated by extranucleosomal DNA. Structures of the regulatory A-module bound to DNA clarify the mechanism of linker DNA binding. The A-module is connected to the motor unit via an HSA/post-HSA lever element to chemomechanically couple the motor and linker DNA sensing. Two notable sites of curved DNA recognition by coordinated action of the four actin/actin-related proteins and the motor suggest how sliding by INO80 can be regulated by extranucleosomal DNA features. Last, the structures clarify the recruitment of YY1/Ies4 subunits and reveal deep architectural similarities between the regulatory modules of INO80 and SWI/SNF complexes.

Item URL in elib:https://elib.dlr.de/194225/
Document Type:Article
Title:Structural mechanism of extranucleosomal DNA readout by the INO80 complex
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iDORCID Put Code
Kunert, FranziskaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Metzner, Felix J.UNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Jung, JamesUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Höpfler, MarkusUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Woike, StephanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Schall, KevinUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Kostrewa, DirkUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Moldt, ManuelaUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Chen, Jia-XuanUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Bantele, SusanneDNA Replication and Genome Integrity, Max Planck Institute of Biochemistry, Martinsried, GermanyUNSPECIFIEDUNSPECIFIED
Pfander, BorisUNSPECIFIEDhttps://orcid.org/0000-0003-2180-5054UNSPECIFIED
Eustermann, SebastianUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Hopfner, Karl-PeterUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Date:9 December 2022
Journal or Publication Title:Science Advances
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:8
DOI:10.1126/sciadv.add3189
Page Range:eadd3189
Publisher:American Association for the Advancement of Science (AAAS)
ISSN:2375-2548
Status:Published
Keywords:extranucleosomal DNA, INO80 and SWI/SNF complexes
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - Radiation & Hypoxia
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:20 Mar 2023 08:51
Last Modified:22 May 2023 11:58

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