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Standardized microbial mock community against pandemic threats

Ly, Y. und Koch, S. und Holtel, J. und Moeller, R. (2022) Standardized microbial mock community against pandemic threats. VAAM, Annual Conference 2022 of the Association for General and Applied Microbiology, 21.–23. Februar 2022, Düsseldorf/Jülich, Germany (virtual).

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Kurzfassung

Microbial monitoring has become an increasing and essential research interest particularly due to the global SARS-CoV-2 pandemic. Predominantly affected was public transportation, which is a mean of transportation used by millions of people on a daily basis. Mass transit systems represent confined rooms, where transmission of infectious diseases through bioaerosols and surfaces is facilitated. To this date, no authentic, microbial enriched bioaerosol has been used and tested for aerosol distribution research. In this study, an artificial microbial mock community was developed, that consists of surrogates from human-associated bacteria that have been found in the airplane cabin (based on Weiss et al. 2019). For technically safe usage in field studies, it was inactivated by high dose X-Ray irradiation. We aimed it to be highly reproducible; for this reason it consists of commercially available bacterial type strains. Among others, low temperature shelf life and desiccation stress testing was performed as with both X-Ray irradiated and unirradiated mock communities. For analysis, 16S amplicon rRNA sequencing was performed by two independent facilities. The obtained results from amplicon sequencing conducted in two individual sequencing facilities showed that the abundances within the mock communities were similarly distributed, but did not completely match. That observation was made for almost all treatments, which proves that significant abundance shifts can be well detected by 16S rRNA sequencing. After desiccation, the mock community was still detectable with similar abundances as the control. Moreover, testing the stability of the mock community at 4°C over one week, a good shelf life was observed. The findings build a foundation for future studies in interdisciplinary aerosol research, using the developed microbial mock community as a biological tool for various studies in applied bioaerosol research as well as for technical improvement studies. It represents a realistic, yet safe and reproducible product used in bioaerosol research for pandemic research, contributing to the development of optimized ventilation systems, or as reference for the evaluation of decontamination methods.

elib-URL des Eintrags:https://elib.dlr.de/190672/
Dokumentart:Konferenzbeitrag (Poster)
Titel:Standardized microbial mock community against pandemic threats
Autoren:
AutorenInstitution oder E-Mail-AdresseAutoren-ORCID-iDORCID Put Code
Ly, Y.Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Koch, S.Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Holtel, J.NICHT SPEZIFIZIERTNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Moeller, R.Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; ralf.moeller (at) dlr.dehttps://orcid.org/0000-0002-2371-0676NICHT SPEZIFIZIERT
Datum:Februar 2022
Referierte Publikation:Ja
Open Access:Nein
Gold Open Access:Nein
In SCOPUS:Nein
In ISI Web of Science:Nein
Status:veröffentlicht
Stichwörter:Microbial monitoring, decontamination methods,
Veranstaltungstitel:VAAM, Annual Conference 2022 of the Association for General and Applied Microbiology
Veranstaltungsort:Düsseldorf/Jülich, Germany (virtual)
Veranstaltungsart:internationale Konferenz
Veranstaltungsdatum:21.–23. Februar 2022
HGF - Forschungsbereich:Luftfahrt, Raumfahrt und Verkehr
HGF - Programm:Raumfahrt
HGF - Programmthema:Forschung unter Weltraumbedingungen
DLR - Schwerpunkt:Raumfahrt
DLR - Forschungsgebiet:R FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):R - Projekt ISS LIFE 2.0
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Strahlenbiologie
Hinterlegt von: Kopp, Kerstin
Hinterlegt am:29 Nov 2022 09:54
Letzte Änderung:29 Nov 2022 09:54

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