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Hypergravity attenuates Reactivity in Primary Murine Astrocytes

Lichterfeld, Yannick and Kalinski, Laura and Schunk, Sarah and Schmakeit, Theresa and Feles, Sebastian and Frett, Timo and Herrmann, Harald and Hemmersbach, Ruth and Liemersdorf, Christian (2022) Hypergravity attenuates Reactivity in Primary Murine Astrocytes. 27th Biennal Symposium and General Assembly (Elgra), 2022-09-06 - 2022-09-10, Lissabon, Portugal.

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Abstract

Neuronal activity is the key modulator of nearly every aspect of behavior, affecting cognition, learning and memory as well as motion. Alterations or even disruptions of the transmission of synaptic signals are the main cause of many neurological disorders. Lesions to nervous tissues are associated with phenotypic changes mediated by astrocytes becoming reactive. Reactive astrocytes form the basis of astrogliosis and glial scar formation. Astrocyte reactivity is often targeted to inhibit axon dystrophy and thus promote neuronal regeneration. Here, we use increased gravitational (mechanical) loading induced by hypergravity to identify a potential method to modify key features of astrocyte reactivity. We exposed primary murine astrocytes as a model system closely resembling the reactivity phenotype in vivo on custom-built centrifuges for cultivation as well as for livecell imaging under hypergravity conditions in a physiological range (2g and 10g). This resulted in significant changes to astrocyte morphology, behavior and reactivity phenotypes, with the ultimate goal being to enhance neuronal regeneration for novel therapeutic approaches.

Item URL in elib:https://elib.dlr.de/189819/
Document Type:Conference or Workshop Item (Speech)
Title:Hypergravity attenuates Reactivity in Primary Murine Astrocytes
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iDORCID Put Code
Lichterfeld, YannickGerman Aerospace Centre (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, Germanyhttps://orcid.org/0000-0001-8755-9920UNSPECIFIED
Kalinski, LauraGerman Aerospace Centre (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, GermanyUNSPECIFIEDUNSPECIFIED
Schunk, SarahGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, GermanyUNSPECIFIEDUNSPECIFIED
Schmakeit, TheresaGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, GermanyUNSPECIFIEDUNSPECIFIED
Feles, SebastianGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, Germanyhttps://orcid.org/0000-0002-5008-6290UNSPECIFIED
Frett, TimoGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Muscle and Bone Metabolism, Cologne (Köln), Germanyhttps://orcid.org/0000-0002-5572-1177UNSPECIFIED
Herrmann, HaraldUniversity of Erlangen, Institute of Neuropathology, , 91054 Erlangen, GermanyUNSPECIFIEDUNSPECIFIED
Hemmersbach, RuthGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, Germanyhttps://orcid.org/0000-0001-5308-6715UNSPECIFIED
Liemersdorf, ChristianGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, Germanyhttps://orcid.org/0000-0001-8407-5226UNSPECIFIED
Date:September 2022
Refereed publication:Yes
Open Access:Yes
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Status:Published
Keywords:Hypergravity, neuronal activity, astrocytes, synaptic signals, astrogliosis, glial scar formation, axon dystrophy, primary murine astrocytes, centrifuge, live-cell imaging,
Event Title:27th Biennal Symposium and General Assembly (Elgra)
Event Location:Lissabon, Portugal
Event Type:international Conference
Event Start Date:6 September 2022
Event End Date:10 September 2022
Organizer:Elgra
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - NeuroSpace
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Gravitational Biology
Deposited By: Chiodo, Annette
Deposited On:17 Nov 2022 09:20
Last Modified:24 Apr 2024 20:51

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