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Dietary lipid droplet structure in postnatal life improves hepatic energy and lipid metabolism in a mouse model for postnatal programming

Jelenik, Tomas and Kodde, Andrea and Pesta, Dominik and Phielix, Esther and Oosting, Annemarie and Rohbeck, Elisabeth and Dewidar, Bedair and Mastrototaro, Lucia and Trenkamp, Sandra and Keijer, Jaap and van der Beek, Eline and Roden, Michael (2022) Dietary lipid droplet structure in postnatal life improves hepatic energy and lipid metabolism in a mouse model for postnatal programming. Pharmacological Research, 179, p. 106193. Elsevier. doi: 10.1016/j.phrs.2022.106193. ISSN 1043-6618.

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Official URL: https://www.sciencedirect.com/science/article/pii/S1043661822001384?via%3Dihub

Abstract

Early-life diets may have a long-lasting impact on metabolic health. This study tested the hypothesis that an early-life diet with large, phospholipid-coated lipid droplets (Concept) induces sustained improvements of hepatic mitochondrial function and metabolism. Young C57BL/6j mice were fed Concept or control (CTRL) diet from postnatal day 15 (PN15) to PN42, followed by western style (WSD) or standard rodent diet (AIN) until PN98. Measurements comprised body composition, insulin resistance (HOMA-IR), tricarboxylic acid (TCA) cycle- and beta-oxidation-related hepatic oxidative capacity using high-resolution respirometry, mitochondrial dynamics, mediators of insulin resistance (diacylglycerols, DAG) or ceramides) in subcellular compartments as well as systemic oxidative stress. Concept feeding increased TCA cycle-related respiration by 33% and mitochondrial fusion protein-1 by 65% at PN42 (both p 0.05). At PN98, CTRL, but not Concept, mice developed hyperinsulinemia (CTRL/AIN 0.22 ± 0.44 vs. CTRL/WSD 1.49 ± 0.53 nmol/l, p 0.05 and Concept/AIN 0.20 ± 0.38 vs. Concept/WSD 1.00 ± 0.29 nmol/l, n.s.) and insulin resistance after WSD (CTRL/AIN 107 ± 23 vs. CTRL/WSD 738 ± 284, p 0.05 and Concept/AIN 109 ± 24 vs. Concept/WSD 524 ± 157, n.s.). WSD-induced liver weight was 18% lower in adult Concept-fed mice and beta-oxidation-related respiration was 69% higher (p 0.05; Concept/WSD vs. Concept/AIN) along with lower plasma lipid peroxides (CTRL/AIN 4.85 ± 0.28 vs. CTRL/WSD 5.73 ± 0.47 µmol/l, p 0.05 and Concept/AIN 4.49 ± 0.31 vs. Concept/WSD 4.42 ± 0.33 µmol/l, n.s.) and were in part protected from WSD-induced increase in hepatic cytosolic DAG C16:0/C18:1. Early-life feeding of Concept partly protected from WSD-induced insulin resistance and systemic oxidative stress, potentially via changes in specific DAG and mitochondrial function, highlighting the role of early life diets on metabolic health later in life.

Item URL in elib:https://elib.dlr.de/189706/
Document Type:Article
Title:Dietary lipid droplet structure in postnatal life improves hepatic energy and lipid metabolism in a mouse model for postnatal programming
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iD
Jelenik, TomasUNSPECIFIEDUNSPECIFIED
Kodde, AndreaUNSPECIFIEDUNSPECIFIED
Pesta, DominikInstitute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germanyhttps://orcid.org/0000-0002-5089-3586
Phielix, EstherUNSPECIFIEDUNSPECIFIED
Oosting, AnnemarieUNSPECIFIEDUNSPECIFIED
Rohbeck, ElisabethUNSPECIFIEDUNSPECIFIED
Dewidar, BedairUNSPECIFIEDUNSPECIFIED
Mastrototaro, LuciaUNSPECIFIEDUNSPECIFIED
Trenkamp, SandraUNSPECIFIEDUNSPECIFIED
Keijer, JaapUNSPECIFIEDUNSPECIFIED
van der Beek, ElineUNSPECIFIEDUNSPECIFIED
Roden, MichaelUNSPECIFIEDUNSPECIFIED
Date:May 2022
Journal or Publication Title:Pharmacological Research
Refereed publication:Yes
Open Access:Yes
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:179
DOI:10.1016/j.phrs.2022.106193
Page Range:p. 106193
Publisher:Elsevier
ISSN:1043-6618
Status:Published
Keywords:Early life programming; Mitochondrial function; Supramolecular structure of lipid droplets; Milk fat globule membrane; Insulin resistance; Diacylglycerol
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - Muscle Mechanics and Metabolism
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Muscle and Bone Metabolism
Deposited By: Pesta, Dominik
Deposited On:10 Nov 2022 11:37
Last Modified:21 Nov 2022 10:28

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