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Ultraviolet disinfection efficacy test method using bacteria monolayers

Kowalski, Wladyslaw and Moeller, Ralf and Walsh, Thomas J. and Petraitis, Vidmantas and Passman, Frederick J. (2022) Ultraviolet disinfection efficacy test method using bacteria monolayers. Journal of Microbiological Methods, 200, p. 106541. Elsevier. doi: 10.1016/j.mimet.2022.106541. ISSN 0167-7012.

Full text not available from this repository.

Official URL: https://dx.doi.org/10.1016/j.mimet.2022.106541

Abstract

Monolayers of bacterial cells of Staphylococcus aureus and Pseudomonas aeruginosa were inoculated on glass slide carriers using an automated inoculum spray deposition system. The use of bacterial monolayers allows for control of critical variables for testing and verification of light-based disinfection technologies. This approach avoids the variability associated with manual inoculation and high inoculum titers, which can engender clustering of cells and the associated photoprotection that clustering incurs. The use of glass slide carriers avoids problems caused by irregular microscopic surface features, which can impact the efficacy evaluation of lightbased disinfection technologies. Scanning electron micrographic (SEM) imaging was used to verify the surface topography and the presence of monolayers. The spray deposition method produced a mean density of >106 colony forming units (CFU) per carrier. The inoculated carriers were exposed to ultraviolet light for 120 s from a focused multivector ultraviolet (FMUV) light system. A mean log CFU reduction of 4.8 was achieved for S. aureus (p < 0.0001). A mean log CFU reduction of 5.1 was achieved for P. aeruginosa (p < 0.0001). The test method presented herein will facilitate increased accuracy in the measurement of ultraviolet susceptibility rate constants.

Item URL in elib:https://elib.dlr.de/188418/
Document Type:Article
Title:Ultraviolet disinfection efficacy test method using bacteria monolayers
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iD
Kowalski, WladyslawSanuvox, Montreal, Quebec, CanadaUNSPECIFIED
Moeller, RalfUNSPECIFIEDhttps://orcid.org/0000-0002-2371-0676
Walsh, Thomas J.Infectious Diseases Translational Research Laboratory, Transplantation-Oncology Infectious Diseases Program, Weill Cornell Medicine of Cornell University, New York City, NY, USAUNSPECIFIED
Petraitis, VidmantasInfectious Diseases Translational Research Laboratory, Transplantation-Oncology Infectious Diseases Program, Weill Cornell Medicine of Cornell University, New York City, NY, USAUNSPECIFIED
Passman, Frederick J.Biodeterioration Control Associates, Inc., Princeton, NJ, USAUNSPECIFIED
Date:21 July 2022
Journal or Publication Title:Journal of Microbiological Methods
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:200
DOI:10.1016/j.mimet.2022.106541
Page Range:p. 106541
Publisher:Elsevier
ISSN:0167-7012
Status:Published
Keywords:Ultraviolet disinfection, FMUV, UV disinfection standard, Monolayer Spray deposition, Automated inoculum deposition
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Transport
HGF - Program Themes:Rail Transport
DLR - Research area:Transport
DLR - Program:V SC Schienenverkehr
DLR - Research theme (Project):V - RoSto - Rolling Stock, V - VMo4Orte - Vernetzte Mobilität für lebenswerte Orte
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:06 Oct 2022 13:37
Last Modified:06 Oct 2022 13:37

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