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Hypergravity attenuates Reactivity in Primary Murine Astrocytes

Lichterfeld, Yannick and Kalinski, Laura and Schunk, Sarah and Schmakeit, Theresa and Feles, Sebastian and Frett, Timo and Herrmann, Harald and Hemmersbach, Ruth and Liemersdorf, Christian (2022) Hypergravity attenuates Reactivity in Primary Murine Astrocytes. Biomedicines, 10 (8), p. 1966. Multidisciplinary Digital Publishing Institute (MDPI). doi: 10.3390/biomedicines10081966. ISSN 2227-9059.

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Official URL: https://www.mdpi.com/2227-9059/10/8/1966

Abstract

Neuronal activity is the key modulator of nearly every aspect of behavior, affecting cognition, learning, and memory as well as motion. Hence, disturbances of the transmission of synaptic signals are the main cause of many neurological disorders. Lesions to nervous tissues are associated with phenotypic changes mediated by astrocytes becoming reactive. Reactive astrocytes form the basis of astrogliosis and glial scar formation. Astrocyte reactivity is often targeted to inhibit axon dystrophy and thus promote neuronal regeneration. Here, we aim to understand the impact of gravitational loading induced by hypergravity to potentially modify key features of astrocyte reactivity. We exposed primary murine astrocytes as a model system closely resembling the in vivo reactivity phenotype on custom-built centrifuges for cultivation as well as for live-cell imaging under hypergravity conditions in a physiological range (2g and 10g). We revealed spreading rates, migration velocities, and stellation to be diminished under 2g hypergravity. In contrast, proliferation and apoptosis rates were not affected. In particular, hypergravity attenuated reactivity induction. We observed cytoskeletal remodeling of actin filaments and microtubules under hypergravity. Hence, the reorganization of these key elements of cell structure demonstrates that fundamental mechanisms on shape and mobility of astrocytes are affected due to altered gravity conditions. In future experiments, potential target molecules for pharmacological interventions that attenuate astrocytic reactivity will be investigated. The ultimate goal is to enhance neuronal regeneration for novel therapeutic approaches

Item URL in elib:https://elib.dlr.de/188275/
Document Type:Article
Title:Hypergravity attenuates Reactivity in Primary Murine Astrocytes
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iDORCID Put Code
Lichterfeld, YannickUNSPECIFIEDhttps://orcid.org/0000-0001-8755-9920UNSPECIFIED
Kalinski, LauraGerman Aerospace Centre (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, GermanyUNSPECIFIEDUNSPECIFIED
Schunk, SarahGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, GermanyUNSPECIFIEDUNSPECIFIED
Schmakeit, TheresaGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, GermanyUNSPECIFIEDUNSPECIFIED
Feles, SebastianGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne (Köln), Germanyhttps://orcid.org/0000-0002-5008-6290UNSPECIFIED
Frett, TimoGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Muscle and Bone Metabolismhttps://orcid.org/0000-0002-5572-1177UNSPECIFIED
Herrmann, HaraldUniversity of Erlangen, Institute of Neuropathology, , 91054 Erlangen, GermanyUNSPECIFIEDUNSPECIFIED
Hemmersbach, RuthGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, Germanyhttps://orcid.org/0000-0001-5308-6715UNSPECIFIED
Liemersdorf, ChristianGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, Germanyhttps://orcid.org/0000-0001-8407-5226UNSPECIFIED
Date:13 August 2022
Journal or Publication Title:Biomedicines
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:10
DOI:10.3390/biomedicines10081966
Page Range:p. 1966
Editors:
EditorsEmailEditor's ORCID iDORCID Put Code
UNSPECIFIEDMDPIUNSPECIFIEDUNSPECIFIED
Publisher:Multidisciplinary Digital Publishing Institute (MDPI)
Series Name:Biomedicines
ISSN:2227-9059
Status:Published
Keywords:neuroscience, primary astrocytes, astrocyte reactivity, astrogliosis, gravitational biology, neuronal regeneration, glial scarring, cytoskeletal remodeling, hypergravity
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - NeuroSpace, R - Gravisensorics
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Gravitational Biology
Deposited By: Chiodo, Annette
Deposited On:22 Sep 2022 09:56
Last Modified:04 Oct 2022 09:29

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