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Early remote detection of downy mildew on grape vine by fluorescence methods

Diedrich, Manuel und Kölbl, Christoph und Ellingen, Elias und Duschek, Frank und Selim, Moustafa und Berkelmann-Löhnertz, Beate (2022) Early remote detection of downy mildew on grape vine by fluorescence methods. XIII. International Symposium on Grapevine Breeding and Genetics 2022, 2022-07-10 - 2022-07-17, Landau.

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Kurzfassung

Pathogenic fungi severely threatens the annual yield of grapes in quantity and quality. Therefore, viticulture requires intensive fungicide applications, compared to other crops. Aimed at a reduction of fungicide input, different techniques and combined strategies are applied in viticulture. To successfully reduce losses in yields, early detection techniques are required. In this work we present premeasurements to remotely detect a pathogenic fungus on the leaves of Vitis vinifera (Müller-Thurgau). We were able to detect a leaf infection with Plasmopara viticola (causal agent of downy mildew) on potted vines within the first week after inoculation. Our results could be reproduced over multiple samples and different methods. Fluorescence mapping was used to monitor the blue-green fluorescence and chlorophyll fluorescence behaviour over a wide area of excitation wavelengths. Based on this, spectral data of fluorescence emission was recorded via fluorometric measurements with excitation at different wavelengths in the visible and UV range. Furthermore, we used laser-induced fluorescence (LIF) and hyperspectral imaging to verify our results. Additionally, we combined hyperspectral imaging with LIF. The evaluation was based on the ratio of blue fluorescence to far-red fluorescence (BFRR_UV) and normalized difference vegetation index (NDVI), which represent an established method in many agricultural applications. Eventually, we evaluated the scalability of our methods for long term measurements. Our results form a fundamental approach for the design of a laser-based stand-off detection system for the practical application in vineyards. Such a system can serve as a model technology for early detection of pathogenic diseases and opens a window for early countermeasures. As mildew infection is considered the most damaging disease in European viticulture, these early countermeasures could greatly reduce the environmental and economic cost associated with fungicide application.

elib-URL des Eintrags:https://elib.dlr.de/187979/
Dokumentart:Konferenzbeitrag (Poster)
Titel:Early remote detection of downy mildew on grape vine by fluorescence methods
Autoren:
AutorenInstitution oder E-Mail-AdresseAutoren-ORCID-iDORCID Put Code
Diedrich, Manuelmanuel.diedrich (at) dlr.dehttps://orcid.org/0000-0002-7512-6500NICHT SPEZIFIZIERT
Kölbl, ChristophChristoph.Koelbl (at) dlr.dehttps://orcid.org/0000-0001-6518-0012NICHT SPEZIFIZIERT
Ellingen, Eliaselias.ellingen (at) dlr.deNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Duschek, FrankFrank.Duschek (at) dlr.dehttps://orcid.org/0000-0002-1809-0257NICHT SPEZIFIZIERT
Selim, MoustafaMoustafa.Selim (at) hs-gm.deNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Berkelmann-Löhnertz, BeateBeate.Berkelmann-Loehnertz (at) hs-gm.deNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Datum:22 Juli 2022
Referierte Publikation:Ja
Open Access:Ja
Gold Open Access:Nein
In SCOPUS:Nein
In ISI Web of Science:Nein
Status:veröffentlicht
Stichwörter:vinolas, remote detection, fluorescence, LIF, hyperspectral imaging, grapevine, pathogenic fungi, downy mildew
Veranstaltungstitel:XIII. International Symposium on Grapevine Breeding and Genetics 2022
Veranstaltungsort:Landau
Veranstaltungsart:internationale Konferenz
Veranstaltungsbeginn:10 Juli 2022
Veranstaltungsende:17 Juli 2022
Veranstalter :Julius-Kühn Institut
HGF - Forschungsbereich:Luftfahrt, Raumfahrt und Verkehr
HGF - Programm:Luftfahrt
HGF - Programmthema:keine Zuordnung
DLR - Schwerpunkt:Luftfahrt
DLR - Forschungsgebiet:L - keine Zuordnung
DLR - Teilgebiet (Projekt, Vorhaben):L - keine Zuordnung
Standort: Lampoldshausen
Institute & Einrichtungen:Institut für Technische Physik > Atmosphärische Propagation und Wirkung
Hinterlegt von: Kölbl, Dr. Christoph
Hinterlegt am:05 Sep 2022 10:05
Letzte Änderung:24 Apr 2024 20:49

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