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Streamlining Culture Conditions for the Neuroblastoma Cell Line SH-SY5Y: A Prerequisite for Functional Studies

Feles, Sebastian and Overath, Christian and Reichardt, Sina and Diegeler, Sebastian and Schmitz, Claudia and Kronenberg, Jessica and Baumstark-Khan, Christa and Hemmersbach, Ruth and Hellweg, Christine Elisabeth and Liemersdorf, Christian (2022) Streamlining Culture Conditions for the Neuroblastoma Cell Line SH-SY5Y: A Prerequisite for Functional Studies. Methods and Protocols, 5 (4), p. 58. Multidisciplinary Digital Publishing Institute (MDPI). doi: 10.3390/mps5040058. ISSN 2409-9279.

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Official URL: https://dx.doi.org/10.3390/mps5040058

Abstract

The neuroblastoma cell line SH-SY5Y has been a well-established and very popular in vitro model in neuroscience for decades, especially focusing on neurodevelopmental disorders, such as Parkinson’s disease. The ability of this cell type to differentiate compared with other models in neurobiology makes it one of the few suitable models without having to rely on a primary culture of neuronal cells. Over the years, various, partly contradictory, methods of cultivation have been reported. This study is intended to provide a comprehensive guide to the in vitro cultivation of undifferentiated SH-SY5Y cells. For this purpose, the morphology of the cell line and the differentiation of the individual subtypes are described, and instructions for cell culture practice and long-term cryoconservation are provided. We describe the key growth characteristics of this cell line, including proliferation and confluency data, optimal initial seeding cell numbers, and a comparison of different culture media and cell viability during cultivation. Furthermore, applying an optimized protocol in a long-term cultivation over 60 days, we show that cumulative population doubling (CPD) is constant over time and does not decrease with incremental passage, enabling stable cultivation, for example, for recurrent differentiation to achieve the highest possible reproducibility in subsequent analyses. Therefore, we provide a solid guidance for future research that employs the neuroblastoma cell line SH-SY5Y

Item URL in elib:https://elib.dlr.de/187772/
Document Type:Article
Title:Streamlining Culture Conditions for the Neuroblastoma Cell Line SH-SY5Y: A Prerequisite for Functional Studies
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iD
Feles, SebastianGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne (Köln), Germanyhttps://orcid.org/0000-0002-5008-6290
Overath, ChristianUniversity Hospital of Cologne, Institute of Virology, Cologne, GermanyUNSPECIFIED
Reichardt, SinaGerman Aerospace Center (DLR), Microgravity User Support Center (MUSC), Cologne, GermanyUNSPECIFIED
Diegeler, SebastianGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Radiation Biology, Cologne, Germanyhttps://orcid.org/0000-0003-3161-0744
Schmitz, ClaudiaGerman Aerospace Centre (DLR), Institute of Aerospace Medicine, Radiation Biology, Cologne, GermanyUNSPECIFIED
Kronenberg, JessicaGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Radiation Biology, Cologne, GermanyUNSPECIFIED
Baumstark-Khan, ChristaGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Radiation Biology, Cologne, Germanyhttps://orcid.org/0000-0002-9329-0128
Hemmersbach, RuthGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne (Köln), Germanyhttps://orcid.org/0000-0001-5308-6715
Hellweg, Christine ElisabethGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Radiation Biology, Cologne, Germanyhttps://orcid.org/0000-0002-2223-3580
Liemersdorf, ChristianGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne (Köln), Germanyhttps://orcid.org/0000-0001-8407-5226
Date:12 July 2022
Journal or Publication Title:Methods and Protocols
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:5
DOI:10.3390/mps5040058
Page Range:p. 58
Publisher:Multidisciplinary Digital Publishing Institute (MDPI)
ISSN:2409-9279
Status:Published
Keywords:neuroscience, in vitro methods, SH-SY5Y, proliferation, characterization, neuroblastoma, morphology, cultivation techniques, cell culture
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - NeuroSpace
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Gravitational Biology
Institute of Aerospace Medicine > Radiation Biology
Space Operations and Astronaut Training > User center for space experiments (MUSC)
Deposited By: Chiodo, Annette
Deposited On:15 Aug 2022 12:35
Last Modified:18 Oct 2022 12:21

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