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Effects of Ionizing Radiation and Long-Term Storage on Hydrated vs. Dried Cell Samples of Extremophilic Microorganisms

Romano, I. and Camerlingo, C. and Vaccari, L. and Birarda, G. and Poli, A. and Fujimori, A. and Lepore, M. and Moeller, R. and Di Donato, P. (2022) Effects of Ionizing Radiation and Long-Term Storage on Hydrated vs. Dried Cell Samples of Extremophilic Microorganisms. Microorganisms, 10 (1), p. 190. Multidisciplinary Digital Publishing Institute (MDPI). doi: 10.3390/microorganisms10010190. ISSN 2076-2607.

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Official URL: http://www.doi.org/10.3390/microorganisms10010190

Abstract

A main factor hampering life in space is represented by high atomic number nuclei and energy (HZE) ions that constitute about 1% of the galactic cosmic rays. In the frame of the “STARLIFE” project, we accessed the Heavy Ion Medical Accelerator (HIMAC) facility of the National Institute of Radiological Sciences (NIRS) in Chiba, Japan. By means of this facility, the extremophilic species Haloterrigena hispanica and Parageobacillus thermantarcticus were irradiated with high LET ions (i.e., Fe, Ar, and He ions) at doses corresponding to long permanence in the space environment. The survivability of HZE-treated cells depended upon either the storage time and the hydration state during irradiation; indeed, dry samples were shown to be more resistant than hydrated ones. With particular regard to spores of the species P. thermantarcticus, they were the most resistant to irradiation in a water medium: an analysis of the changes in their biochemical fingerprinting during irradiation showed that, below the survivability threshold, the spores undergo to a germination-like process, while for higher doses, inactivation takes place as a consequence of the concomitant release of the core’s content and a loss of integrity of the main cellular components. Overall, the results reported here suggest that the selected extremophilic microorganisms could serve as biological model for space simulation and/or real space condition exposure, since they showed good resistance to ionizing radiation exposure and were able to resume cellular growth after long-term storage.

Item URL in elib:https://elib.dlr.de/187623/
Document Type:Article
Title:Effects of Ionizing Radiation and Long-Term Storage on Hydrated vs. Dried Cell Samples of Extremophilic Microorganisms
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iD
Romano, I.Institute of Biomolecular Chemistry, National Research Council of Italy, Via Campi Flegrei, 34, 80078 Pozzuoli, ItalyUNSPECIFIED
Camerlingo, C.SuPerconducting and Other INnovative Materials and Devices Institute, National Research Council of Italy, Via Campi Flegrei, 34, 80078 Pozzuoli, ItalyUNSPECIFIED
Vaccari, L.Elettra—Sincrotrone Trieste S.C.p.A. S.S., 14 km 163,5 in Area Science Park, Basovizza, 34149 Trieste, ItalyUNSPECIFIED
Birarda, G.Elettra—Sincrotrone Trieste S.C.p.A. S.S., 14 km 163,5 in Area Science Park, Basovizza, 34149 Trieste, ItalyUNSPECIFIED
Poli, A.Institute of Biomolecular Chemistry, National Research Council of Italy, Via Campi Flegrei, 34, 80078 Pozzuoli, ItalyUNSPECIFIED
Fujimori, A.National Institutes for Quantum and Radiological Science and Technology (NIRS/QST), Department of Basic Medical Sciences for Radiation Damages, Chiba, Japan.UNSPECIFIED
Lepore, M.Dipartimento di Medicina Sperimentale, Università della Campania “L. Vanvitelli”, Via S. Maria di Costantinopoli 16, 80138 Napoli, ItalyUNSPECIFIED
Moeller, R.Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; ralf.moeller (at) dlr.dehttps://orcid.org/0000-0002-2371-0676
Di Donato, P.Institute of Biomolecular Chemistry, National Research Council of Italy, Via Campi Flegrei, 34, 80078 Pozzuoli, ItalyUNSPECIFIED
Date:16 January 2022
Journal or Publication Title:Microorganisms
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:10
DOI:10.3390/microorganisms10010190
Page Range:p. 190
Publisher:Multidisciplinary Digital Publishing Institute (MDPI)
ISSN:2076-2607
Status:Published
Keywords:extremophiles; space radiation environment; biochemical fingerprinting; STARLIFE project; SERS; IR microspectroscopy
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - Project ISS LIFE 2.0
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine
Institute of Aerospace Medicine > Radiation Biology
Deposited By: Schrage, Larissa
Deposited On:15 Aug 2022 12:34
Last Modified:22 Sep 2022 11:35

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