elib
DLR-Header
DLR-Logo -> http://www.dlr.de
DLR Portal Home | Imprint | Privacy Policy | Contact | Deutsch
Fontsize: [-] Text [+]

Hypergravity Reduces Astrocyte Migration by Altering Cytoskeletal Dynamics

Liemersdorf, Christian and Lichterfeld, Yannick and Kalinski, Laura and Frett, Timo and Jordan, Jens and Hemmersbach, Ruth (2020) Hypergravity Reduces Astrocyte Migration by Altering Cytoskeletal Dynamics. FASEB JOURNAL, 34 (S1). Federation of American Societies for Experimental Biology. doi: 10.1096/fasebj.2020.34.s1.02872. ISSN 0892-6638.

Full text not available from this repository.

Abstract

Glial scar formation through astrocytes impairs neural regeneration following spinal cord injury, head trauma, or stroke. Astrocyte migration towards the lesion and induction of a reactive astroglial phenotype require dynamic cytoskeletal protein rearrangements. In this glial cell model, hypergravity was used to alter cytoskeletal dynamics, i.e. stabilizing microtubules while destabilizing actin filaments. We hypothesize that increased gravitational (mechanical) loading by means of centrifugation (hypergravity) modulates in vitro astrocyte function in a way that could reduce their potential for scar formation. We exposed primary murine cortical astrocytes to 2g using two types of custom-designed hypergravity platforms at DLR (Cologne, Germany) and assessed a variety of parameters important for glial scarring in vitro. The platforms unlike commercial laboratory centrifuges model physiological hypergravity and allow for cell cultivation and live-cell imaging. Primary astrocytes were isolated from wildtype (C57BL/6J) as well as transgenic LifeAct-GFP mice and subjected to increased gravitational load. We measured cell proliferation and survival, after 7 days of exposure to 2g, as well as spreading and migration rate online for 24h. We visualized morphological features, cytoskeletal actin filament dynamics, reactivity markers and investigated expression levels of focal adhesion-related proteins. The exposure to 2g hypergravity induced a decrease in cell spreading (20%) coincidental with an inhibited migratory behavior (40%) and altered cytoskeletal dynamics. Astrocytic proliferation and survival were not affected. The expression of the focal adhesion marker vinculin was increased by 70–80%. We conclude that hypergravity attenuates astrocyte spreading and migration. These parameters are crucial for glial scar formation, while basic cellular processes, such as proliferation and apoptosis were unchanged. The response appears to be mediated through altered cytoskeletal dynamics and may provide targets for therapies promoting neuronal regeneration.

Item URL in elib:https://elib.dlr.de/185770/
Document Type:Article
Title:Hypergravity Reduces Astrocyte Migration by Altering Cytoskeletal Dynamics
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iD
Liemersdorf, ChristianChristian.Liemersdorf (at) dlr.deUNSPECIFIED
Lichterfeld, YannickYannick.Lichterfeld (at) dlr.deUNSPECIFIED
Kalinski, LauraLaura.Kalinski (at) dlr.deUNSPECIFIED
Frett, TimoTimo.Frett (at) dlr.dehttps://orcid.org/0000-0002-5572-1177
Jordan, JensInstitute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany and Lehrstuhl für Luft- und Raumfahrtmedizin, Universität zu Köln; Jens.Jordan (at) dlr.dehttps://orcid.org/0000-0003-4518-0706
Hemmersbach, RuthRuth.Hemmersbach (at) dlr.deUNSPECIFIED
Date:2020
Journal or Publication Title:FASEB JOURNAL
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:34
DOI :10.1096/fasebj.2020.34.s1.02872
Publisher:Federation of American Societies for Experimental Biology
ISSN:0892-6638
Status:Published
Keywords:Hypergravity; Astrocyte; Cytoskeletal Dynamic
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - Human performance under altered gravity conditions
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Gravitational Biology
Deposited By: Becker, Christine
Deposited On:11 Apr 2022 10:20
Last Modified:11 Apr 2022 10:20

Repository Staff Only: item control page

Browse
Search
Help & Contact
Information
electronic library is running on EPrints 3.3.12
Copyright © 2008-2017 German Aerospace Center (DLR). All rights reserved.