elib
DLR-Header
DLR-Logo -> http://www.dlr.de
DLR Portal Home | Imprint | Privacy Policy | Contact | Deutsch
Fontsize: [-] Text [+]

The impact of microgravity and gravitational countermeasures on the gut microbiome of humans enrolled in the AGBRESA study

Himmelmann, Alexandra and Ramos-Nascimento, Ana and Frings-Meuthen, Petra and Mulder, Edwin and Hellweg, Christine E. and Moeller, Ralf and and the, AGBRESA gut microbiome research team (2021) The impact of microgravity and gravitational countermeasures on the gut microbiome of humans enrolled in the AGBRESA study. 59. Wissenschaftliche Jahrestagung Deutsche Gesellschaft für Luft- und Raumfahrtmedizin (DGLRM) e. V., 4. Dezember 2021, Virtual Conference.

[img] PDF
81kB

Abstract

The Artificial Gravity Bed Rest Study – AGBRESA – was the first joint study conducted by DLR, ESA and NASA to simulate the effects of microgravity on healthy subjects. Moreover, the study included the use of artificial gravity protocols in a short-arm human centrifuge as a measure to counteract the negative effects of weightlessness. The health of the gut translates into the overall wellbeing since the disruption of the gut symbiotic networks – dysbiosis – could be due to either diet, antibiotic ingestion, sleep disturbance, physical activity or psychological stresses. In recent times, the gut microbiome has changed from being a complementary addition to our digestive tract to a potentially life-changing role by directly being the source of stimuli which revealed to impact neurochemistry, behavior and overall physiological status. Combined, microbial fluctuations could alter the intestinal microbiota composition and bacterial metabolite production, or more severely, in the disruption of host intestinal barrier integrity and the immune system activity, triggering intestinal inflammation syndromes and making the gut a very relevant organ to be studied in the context of spaceflight. Thus, 12 subjects, 8 males, were subjected to bed rest at negative 6-degree inclination for a period of 60 days with a preceding baseline of 15 days and posterior recovery period of 14 days. In other to characterize the gut microenvironment of healthy humans in simulated microgravity, fecal samples were collected during the baseline stage (once), during the head-down tilt treatment (at days 10, 30, and 50) and during the recovery period (once), and the samples were then processed for 16S rRNA sequencing and taxonomic analysis of the gut microenvironment. The characterization of the prokaryote flora was conducted 1) throughout time in contrast to the baseline reference and 2) in the context of the gravitational countermeasure vs the bed-rest-only control. The analysis revealed the detection of commensal microorganisms described to positively impact the gut such as Bifidobacterium spp., Lactobacillus spp., Akkermansia spp. and Enterococus spp.. Interestingly, we were able to detect pathogens like Campylobacter hominis which has been linked to severe bowel diseases ulcerative colitis and Crohn's disease. Also, opportunistic microorganisms such as Fusobacterium spp., Prevotella spp., Pseudomonas spp., Staphylococcus and Streptococcus spp., could potentially indicate an imbalance of the microbial networks and be a good an indicator of dysbiosis. Additionally, we set aside samples to undergo proteomic and metabolite analysis to improve the characterization of the gut microenvironment under microgravity simulation and the extent of the gravitational countermeasure recovery on bowel condition. Overall, the microgravity simulation performed on the AGBRESA study did not impact dramatically the fitness of the participants. Nonetheless, the analysis of the gut provides important insights on the triggers that occur during the adaptation of human physiology to long term exposure to spaceflight conditions and whether these relate to the described complications associated with gut disease.

Item URL in elib:https://elib.dlr.de/147103/
Document Type:Conference or Workshop Item (Speech)
Title:The impact of microgravity and gravitational countermeasures on the gut microbiome of humans enrolled in the AGBRESA study
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iD
Himmelmann, AlexandraInstitute of Aerospace Medicine, German Aerospace Center (DLR e.V.)UNSPECIFIED
Ramos-Nascimento, AnaInstitute of Aerospace Medicine, German Aerospace Center (DLR e.V.)UNSPECIFIED
Frings-Meuthen, PetraPetra.Frings-Meuthen (at) dlr.deUNSPECIFIED
Mulder, EdwinEdwin.Mulder (at) dlr.deUNSPECIFIED
Hellweg, Christine E.Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; Christine.Hellweg (at) dlr.dehttps://orcid.org/0000-0002-2223-3580
Moeller, RalfRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; ralf.moeller (at) dlr.de and Natural Sciences Department, University of Applied Sciences Bonn-Rhein-Sieg (BRSU), D-53359 Rheinbach, Germanyhttps://orcid.org/0000-0002-2371-0676
and the, AGBRESA gut microbiome research teamUNSPECIFIEDUNSPECIFIED
Date:December 2021
Refereed publication:Yes
Open Access:Yes
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Status:Published
Keywords:The Artificial Gravity Bed Rest Study – AGBRESA
Event Title:59. Wissenschaftliche Jahrestagung Deutsche Gesellschaft für Luft- und Raumfahrtmedizin (DGLRM) e. V.
Event Location:Virtual Conference
Event Type:national Conference
Event Dates:4. Dezember 2021
Organizer:Deutsche Gesellschaft für Luft- und Raumfahrtmedizin e.V.
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - Project ISS LIFE 2.0
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Institute of Aerospace Medicine > Muscle and Bone Metabolism
Institute of Aerospace Medicine > Leitungsbereich ME
Deposited By: Kopp, Kerstin
Deposited On:14 Dec 2021 14:57
Last Modified:14 Dec 2021 14:57

Repository Staff Only: item control page

Browse
Search
Help & Contact
Information
electronic library is running on EPrints 3.3.12
Copyright © 2008-2017 German Aerospace Center (DLR). All rights reserved.