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The effect of revascularization on recovery of mitochondrial respiration in peripheral artery disease: a case control study

Gratl, Alexandra and Pesta, Dominik and Gruber, Leonhard and Speichinger, Fiona and Raude, Ben and Omran, Safwan and Greiner, Andreas and Frese, Jan Paul (2021) The effect of revascularization on recovery of mitochondrial respiration in peripheral artery disease: a case control study. Journal of Translational Medicine, 19 (1), p. 244. Springer Nature. doi: 10.1186/s12967-021-02908-0. ISSN 1479-5876.

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Official URL: http://dx.doi.org/10.1186/s12967-021-02908-0

Abstract

Background: Peripheral arterial disease (PAD) is accompanied by myopathy characterized by mitochondrial dysfunction. The aim of this experimental study was to investigate the effect of revascularization procedures on mitochondrial function in ischemic and non-ischemic muscle. Methods: Muscle biopsies from patients with symptomatic stage IIB/III PAD caused by isolated pathologies of the superficial femoral artery were obtained from muscle regions within the chronic ischemic muscle (gastrocnemius) and from non-ischemic muscle (vastus lateralis) before and 6 weeks after invasive revascularization. High-resolution respirometry was used to investigate mitochondrial function and results were normalized to citrate synthase activity (CSA). Results are given in absolute values and fold over basal (FOB). Results: Respiratory states (OXPHOS (P) and electron transfer (E) capacity) normalized to CSA decreased while CSA was increased in chronic ischemic muscle after revascularization. There were no changes in in non-ischemic muscle. The FOB of chronic ischemic muscle was significantly higher for CSA (chronic ischemic 1.37 (IQR 1.10-1.64) vs. non-ischemic 0.93 (IQR 0.69-1.16) p = 0.020) and significantly lower for respiratory states normalized to CSA when compared to the non-ischemic muscle (P per CSA chronic ischemic 0.64 (IQR 0.46-0.82) vs non-ischemic 1.16 (IQR 0.77-1.54) p = 0.011; E per CSA chronic ischemic 0.61 (IQR 0.47-0.76) vs. non-ischemic 1.02 (IQR 0.64-1.40) p = 0.010). Conclusions: Regeneration of mitochondrial content and function following revascularization procedures only occur in muscle regions affected by malperfusion. This indicates that the restoration of blood and oxygen supply are important mediators aiding mitochondrial recovery.

Item URL in elib:https://elib.dlr.de/144669/
Document Type:Article
Title:The effect of revascularization on recovery of mitochondrial respiration in peripheral artery disease: a case control study
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iD
Gratl, AlexandraUNSPECIFIEDUNSPECIFIED
Pesta, DominikInstitute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; Dominik.Pesta (at) dlr.dehttps://orcid.org/0000-0002-5089-3586
Gruber, LeonhardUNSPECIFIEDUNSPECIFIED
Speichinger, FionaUNSPECIFIEDUNSPECIFIED
Raude, BenUNSPECIFIEDUNSPECIFIED
Omran, SafwanUNSPECIFIEDUNSPECIFIED
Greiner, AndreasUNSPECIFIEDUNSPECIFIED
Frese, Jan PaulUNSPECIFIEDUNSPECIFIED
Date:4 June 2021
Journal or Publication Title:Journal of Translational Medicine
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:19
DOI :10.1186/s12967-021-02908-0
Page Range:p. 244
Publisher:Springer Nature
ISSN:1479-5876
Status:Published
Keywords:Intermittent claudication; Mitochondrial function; Mitochondrial respiration; PAD; Peripheral arterial disease; Revascularization
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - Bone metabolism and structural adaptation
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Muscle and Bone Metabolism
Deposited By: Schönenberg, Sandra
Deposited On:28 Oct 2021 16:04
Last Modified:29 Oct 2021 12:34

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