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Human Platelet Mitochondrial Function Reflects Systemic Mitochondrial Alterations: A Protocol for Application in Field Studies

Hoppel, Florian and Garcia-Souza, Luiz Felipe and Kantner-Rumplmair, Wilhelm and Burtscher, Martin and Gnaiger, Erich and Pesta, Dominik and Calabria, Elisa (2021) Human Platelet Mitochondrial Function Reflects Systemic Mitochondrial Alterations: A Protocol for Application in Field Studies. Cells, 10 (8), p. 2088. Multidisciplinary Digital Publishing Institute (MDPI). doi: 10.3390/cells10082088. ISSN 2073-4409.

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Official URL: http://dx.doi.org/10.3390/cells10082088

Abstract

Human blood cells may offer a minimally invasive strategy to study systemic alterations of mitochondrial function. Here we tested the reliability of a protocol designed to study mitochondrial respiratory control in human platelets (PLTs) in field studies, using high-resolution respirometry (HRR). Several factors may trigger PLT aggregation during the assay, altering the homogeneity of the cell suspension and distorting the number of cells added to the two chambers (A, B) of the Oroboros Oxygraph-2k (O2k). Thus, inter-chamber variability (∆ab) was calculated by normalizing oxygen consumption to chamber volume (JO2) or to a specific respiratory control state (flux control ratio, FCR) as a reliable parameter of experimental quality. The method’s reliability was tested by comparing the ∆ab of laboratory-performed experiments (LAB, N = 9) to those of an ultramarathon field study (three sampling time-points: before competition (PRE, N = 7), immediately after (POST, N = 10) and 24 h after competition (REC; N = 10)). Our results show that ∆ab JO2 changed PRE-POST, but also for LAB-POST and LAB-REC, while all ∆ab FCR remained unchanged. Thus, we conclude that our method is reliable for assessing PLT mitochondrial function in LAB and field studies and after systemic stress conditions.

Item URL in elib:https://elib.dlr.de/144664/
Document Type:Article
Title:Human Platelet Mitochondrial Function Reflects Systemic Mitochondrial Alterations: A Protocol for Application in Field Studies
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iD
Hoppel, FlorianUNSPECIFIEDUNSPECIFIED
Garcia-Souza, Luiz FelipeUNSPECIFIEDUNSPECIFIED
Kantner-Rumplmair, WilhelmUNSPECIFIEDUNSPECIFIED
Burtscher, MartinDepartment of Sport Science, University of Innsbruck, A-6020 Innsbruck, Austria;Martin.Burtscher (at) uibk.ac.athttps://orcid.org/0000-0002-5232-3632
Gnaiger, ErichUNSPECIFIEDUNSPECIFIED
Pesta, DominikInstitute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; Dominik.Pesta (at) dlr.dehttps://orcid.org/0000-0002-5089-3586
Calabria, ElisaDepartment of Neurosciences, Biomedicine and Movement Sciences, University of Verona, 37131 Verona, Italy; a.calabria (at) univr.ithttps://orcid.org/0000-0001-6557-0379
Date:14 August 2021
Journal or Publication Title:Cells
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:10
DOI :10.3390/cells10082088
Page Range:p. 2088
Publisher:Multidisciplinary Digital Publishing Institute (MDPI)
ISSN:2073-4409
Status:Published
Keywords:platelets; respirometry; field study; physical exercise; quality control; mitochondrial function
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - Bone metabolism and structural adaptation
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Muscle and Bone Metabolism
Deposited By: Schönenberg, Sandra
Deposited On:28 Oct 2021 15:49
Last Modified:29 Oct 2021 12:31

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