Bauer, Johann and Gombocz, Erich and Schulz, Herbert and Hauslage, Jens and Grimm, Daniela (2021) Interaction Network Provides Clues on the Role of BCAR1 in Cellular Response to Changes in Gravity. Computation, 9 (8), p. 81. Multidisciplinary Digital Publishing Institute (MDPI). doi: 10.3390/computation9080081. ISSN 2079-3197.
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Official URL: http://dx.doi.org/10.3390/computation9080081
Abstract
When culturing cells in space or under altered gravity conditions on Earth to investigate the impact of gravity, their adhesion and organoid formation capabilities change. In search of a target where the alteration of gravity force could have this impact, we investigated p130cas/BCAR1 and its interactions more thoroughly, particularly as its activity is sensitive to applied forces. This protein is well characterized regarding its role in growth stimulation and adhesion processes. To better understand BCAR1′s force-dependent scaffolding of other proteins, we studied its interactions with proteins we had detected by proteome analyses of MCF-7 breast cancer and FTC-133 thyroid cancer cells, which are both sensitive to exposure to microgravity and express BCAR1. Using linked open data resources and our experiments, we collected comprehensive information to establish a semantic knowledgebase and analyzed identified proteins belonging to signaling pathways and their networks. The results show that the force-dependent phosphorylation and scaffolding of BCAR1 influence the structure, function, and degradation of intracellular proteins as well as the growth, adhesion and apoptosis of cells similarly to exposure of whole cells to altered gravity. As BCAR1 evidently plays a significant role in cell responses to gravity changes, this study reveals a clear path to future research performing phosphorylation experiments on BCAR1.
Item URL in elib: | https://elib.dlr.de/144055/ | ||||||||||||||||||||||||
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Document Type: | Article | ||||||||||||||||||||||||
Title: | Interaction Network Provides Clues on the Role of BCAR1 in Cellular Response to Changes in Gravity | ||||||||||||||||||||||||
Authors: |
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Date: | 23 July 2021 | ||||||||||||||||||||||||
Journal or Publication Title: | Computation | ||||||||||||||||||||||||
Refereed publication: | Yes | ||||||||||||||||||||||||
Open Access: | Yes | ||||||||||||||||||||||||
Gold Open Access: | Yes | ||||||||||||||||||||||||
In SCOPUS: | Yes | ||||||||||||||||||||||||
In ISI Web of Science: | Yes | ||||||||||||||||||||||||
Volume: | 9 | ||||||||||||||||||||||||
DOI: | 10.3390/computation9080081 | ||||||||||||||||||||||||
Page Range: | p. 81 | ||||||||||||||||||||||||
Publisher: | Multidisciplinary Digital Publishing Institute (MDPI) | ||||||||||||||||||||||||
ISSN: | 2079-3197 | ||||||||||||||||||||||||
Status: | Published | ||||||||||||||||||||||||
Keywords: | knowledge explorer, pathway studio, mass spectrometry, post-translational modification, network analysis, SPARQL | ||||||||||||||||||||||||
HGF - Research field: | Aeronautics, Space and Transport | ||||||||||||||||||||||||
HGF - Program: | Space | ||||||||||||||||||||||||
HGF - Program Themes: | Research under Space Conditions | ||||||||||||||||||||||||
DLR - Research area: | Raumfahrt | ||||||||||||||||||||||||
DLR - Program: | R FR - Research under Space Conditions | ||||||||||||||||||||||||
DLR - Research theme (Project): | R - Life Sciences Experiments Moon | ||||||||||||||||||||||||
Location: | Köln-Porz | ||||||||||||||||||||||||
Institutes and Institutions: | Institute of Aerospace Medicine > Gravitational Biology | ||||||||||||||||||||||||
Deposited By: | Hauslage, Jens | ||||||||||||||||||||||||
Deposited On: | 06 Oct 2021 14:18 | ||||||||||||||||||||||||
Last Modified: | 05 Dec 2023 09:35 |
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