DLR-Logo -> http://www.dlr.de
DLR Portal Home | Imprint | Privacy Policy | Contact | Deutsch
Fontsize: [-] Text [+]

Interaction Network Provides Clues on the Role of BCAR1 in Cellular Response to Changes in Gravity

Bauer, Johann and Gombocz, Erich and Schulz, Herbert and Hauslage, Jens and Grimm, Daniela (2021) Interaction Network Provides Clues on the Role of BCAR1 in Cellular Response to Changes in Gravity. Computation, 9 (8), p. 81. Multidisciplinary Digital Publishing Institute (MDPI). doi: 10.3390/computation9080081. ISSN 2079-3197.

[img] PDF - Published version

Official URL: http://dx.doi.org/10.3390/computation9080081


When culturing cells in space or under altered gravity conditions on Earth to investigate the impact of gravity, their adhesion and organoid formation capabilities change. In search of a target where the alteration of gravity force could have this impact, we investigated p130cas/BCAR1 and its interactions more thoroughly, particularly as its activity is sensitive to applied forces. This protein is well characterized regarding its role in growth stimulation and adhesion processes. To better understand BCAR1′s force-dependent scaffolding of other proteins, we studied its interactions with proteins we had detected by proteome analyses of MCF-7 breast cancer and FTC-133 thyroid cancer cells, which are both sensitive to exposure to microgravity and express BCAR1. Using linked open data resources and our experiments, we collected comprehensive information to establish a semantic knowledgebase and analyzed identified proteins belonging to signaling pathways and their networks. The results show that the force-dependent phosphorylation and scaffolding of BCAR1 influence the structure, function, and degradation of intracellular proteins as well as the growth, adhesion and apoptosis of cells similarly to exposure of whole cells to altered gravity. As BCAR1 evidently plays a significant role in cell responses to gravity changes, this study reveals a clear path to future research performing phosphorylation experiments on BCAR1.

Item URL in elib:https://elib.dlr.de/144055/
Document Type:Article
Title:Interaction Network Provides Clues on the Role of BCAR1 in Cellular Response to Changes in Gravity
AuthorsInstitution or Email of AuthorsAuthor's ORCID iDORCID Put Code
Hauslage, JensDLR Institute of Aerospace Medicine, Gravitational Biology, Cologne, Germanyhttps://orcid.org/0000-0003-2184-7000UNSPECIFIED
Date:23 July 2021
Journal or Publication Title:Computation
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In ISI Web of Science:Yes
Page Range:p. 81
Publisher:Multidisciplinary Digital Publishing Institute (MDPI)
Keywords:knowledge explorer, pathway studio, mass spectrometry, post-translational modification, network analysis, SPARQL
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - Life Sciences Experiments Moon
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Gravitational Biology
Deposited By: Hauslage, Jens
Deposited On:06 Oct 2021 14:18
Last Modified:05 Dec 2023 09:35

Repository Staff Only: item control page

Help & Contact
electronic library is running on EPrints 3.3.12
Website and database design: Copyright © German Aerospace Center (DLR). All rights reserved.