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Microbial Monitoring in the EDEN ISS Greenhouse, a Mobile Test Facility in Antarctica

Beblo-Vranesevic, K. und Fahrion, J. und Zabel, P. und Schubert, D. und Mysara, M. und Van Houdt, R. und Eikmanns, B. und Rettberg, P. (2021) Microbial Monitoring in the EDEN ISS Greenhouse, a Mobile Test Facility in Antarctica. 23rd IAA Humans in Space, 5.-8. April 2021, Virtual Conference (Moskow, Russia).

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Kurzfassung

The EDEN ISS greenhouse, integrated in two joined containers, is a confined mobile test facility in Antarctica for the development and optimization of new plant cultivation techniques for future space programs. The EDEN ISS greenhouse was used successfully from February to November 2018 for fresh food production for the overwintering crew at the Antarctic Neumayer III station. During the 9 months of operation, samples from the different plants, from the nutrition solution of the aeroponic planting system, and from diverse surfaces within the three different compartments of the container were taken [future exploration greenhouse (FEG), service section (SS), and cold porch (CP)]. Quantity as well as diversity of microorganisms was examined by cultivation. In case of the plant samples, microbial quantities were in a range from 102 to 104 colony forming units (CFU) per gram plant material. Compared to plants purchased from a German grocery, the produce hosted orders of magnitude more microorganisms than the EDEN ISS plants. The EDEN ISS plant samples contained mainly fungi and a few bacteria. No classical food associated pathogenic microorganism, like Escherichia and Salmonella, could be found. Probably due to the used cultivation approach, Archaea were not found in the samples. The bioburden in the nutrition solutions increased constantly over time but never reached critical values like 10² –10³ CFU per 100 mL in irrigation water as it is stated, e.g., for commercial European plant productions. The surface samples revealed high differences in the microbial burden between the greenhouse part of the container and the SS and CP part. However, the numbers of organisms (bacteria and fungi) found in the planted greenhouse were still not critical. The microbial loaded surfaces showed strong temporal as well as spatial fluctuations. In samples of the nutrition solution and the surface, the number of bacteria exceeded the amount of fungi by many times. For identification, 16S rRNA gene sequencing was performed for the isolated prokaryotic organisms. Phylogenetic analyses revealed that the most abundant bacterial phyla were Firmicutes and Actinobacteria. These phyla include plant- and human-associated bacterial species. In general, it could be shown that it is possible to produce edible fresh food in a remote environment and this food is safe for consumption from a microbiological point of view.

elib-URL des Eintrags:https://elib.dlr.de/142846/
Dokumentart:Konferenzbeitrag (Vortrag)
Titel:Microbial Monitoring in the EDEN ISS Greenhouse, a Mobile Test Facility in Antarctica
Autoren:
AutorenInstitution oder E-Mail-AdresseAutoren-ORCID-iDORCID Put Code
Beblo-Vranesevic, K.Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; kristina.beblo (at) dlr.dehttps://orcid.org/0000-0002-4834-7121NICHT SPEZIFIZIERT
Fahrion, J.Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany and Institute of Microbiology and Biotechnology, Faculty of Natural Sciences, University of Ulm, Ulm, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Zabel, P.Institute for Space Systems, German Aerospace Center (DLR), Bremen, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Schubert, D.Institute for Space Systems, German Aerospace Center (DLR), Bremen, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Mysara, M.Microbiology Unit, Belgian Nuclear Research Centre (SCK CEN), Mol, BelgiumNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Van Houdt, R.Microbiology Unit, Belgian Nuclear Research Centre (SCK CEN), Mol, BelgiumNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Eikmanns, B.Institute of Microbiology and Biotechnology, Faculty of Natural Sciences, University of Ulm, Ulm, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Rettberg, P.Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; petra.rettberg (at) dlr.dehttps://orcid.org/0000-0003-4439-2395NICHT SPEZIFIZIERT
Datum:6 April 2021
Referierte Publikation:Ja
Open Access:Ja
Gold Open Access:Nein
In SCOPUS:Nein
In ISI Web of Science:Nein
Status:veröffentlicht
Stichwörter:EDEN ISS greenhouse, Antarctica, microbial monitoring
Veranstaltungstitel:23rd IAA Humans in Space
Veranstaltungsort:Virtual Conference (Moskow, Russia)
Veranstaltungsart:internationale Konferenz
Veranstaltungsdatum:5.-8. April 2021
HGF - Forschungsbereich:Luftfahrt, Raumfahrt und Verkehr
HGF - Programm:Raumfahrt
HGF - Programmthema:Forschung unter Weltraumbedingungen
DLR - Schwerpunkt:Raumfahrt
DLR - Forschungsgebiet:R FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):R - Projekt ISS LIFE 2.0
Standort: Bremen , Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Strahlenbiologie
Institut für Raumfahrtsysteme > Systemanalyse Raumsegment
Hinterlegt von: Kopp, Kerstin
Hinterlegt am:22 Jun 2021 12:54
Letzte Änderung:22 Jun 2021 13:13

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