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Evaluation of State-of-the-Art Decontamination Techniques and Innovative Prevention Methods on the Astrobiological Model Organism Aspergillus niger

Iannicelli Caiaffa, Manoela (2021) Evaluation of State-of-the-Art Decontamination Techniques and Innovative Prevention Methods on the Astrobiological Model Organism Aspergillus niger. Bachelor's, University of Applied Sciences Bonn-Rhein-Sieg.

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Abstract

Aspergillus niger represents one of the major fungal contaminants inside the International Space Station (ISS). It represents a hazard for human health and can compromise structural installations. It can act as an opportunistic pathogen for humans and can cause a wide variety of issues, such as skin and respiratory tract infections. It affects materials and built structures by excessive growth by releasing secondary metabolites which cause biocorrosion of materials. Fungal spores, the dispersal units and focus of this study have been observed to survive the high doses of radiation and show resistance against most of the commercial sterilization techniques. In this thesis, we tested the state-of-the-art decontamination methods: Autoclavation, UV-C irradiation, and Hydrogen peroxide (H₂​O₂​), using two strains of ​A. niger​. Corresponding results show that the wildtype strain was more capable of survival than a counterpart mutant strain with a melanin deficiency. Secondly, investigations of preventing a manifestation of fungal contamination within a closed built environment, specifically structured copper and copper-containing surfaces were tested for their effect on the viability of spores with similar results. In a nutshell, this thesis demonstrated that ​A. niger spores are capable of surviving harsh conditions, as well as the need for standardization of decontamination techniques. In conclusion, ​A. niger spores should be considered standard sterilization indicator organisms that can be used to indicate sterility in the construction of future equipment to be used in long-term space missions or any other installations that require sterile conditions.

Item URL in elib:https://elib.dlr.de/141935/
Document Type:Thesis (Bachelor's)
Title:Evaluation of State-of-the-Art Decontamination Techniques and Innovative Prevention Methods on the Astrobiological Model Organism Aspergillus niger
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iD
Iannicelli Caiaffa, ManoelaRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany.UNSPECIFIED
Date:April 2021
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Number of Pages:88
Status:Published
Keywords:state-of-the-art decontamination methods, Aspergillus niger, Astrobiology
Institution:University of Applied Sciences Bonn-Rhein-Sieg
Department:Department of Natural Sciences
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Aeronautics
HGF - Program Themes:Air Transportation and Impact
DLR - Research area:Aeronautics
DLR - Program:L AI - Air Transportation and Impact
DLR - Research theme (Project):L - Human Factors, R - Project ISS LIFE 2.0
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:28 May 2021 11:00
Last Modified:28 May 2021 11:00

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