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THE MICROBIOME OF A GREENHOUSE FOR GROUND DEMONSTRATION OF PLANT CULTIVATION TECHNOLOGIES IN SPACE

Rettberg, Petra and Fahrion, Jana and Zabel, Paul and Schubert, Daniel (2021) THE MICROBIOME OF A GREENHOUSE FOR GROUND DEMONSTRATION OF PLANT CULTIVATION TECHNOLOGIES IN SPACE. COSPAR 2021, 28. Januar - 4. Februar 2021, Sydney, Australia and Virtual Event (Hybrid).

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Abstract

The EDEN ISS greenhouse is a confined mobile test facility in Antarctica for the development and optimization of new plant cultivation techniques for future space programs. The EDEN ISS greenhouse was in operation from February to November 2018 for fresh food production for the overwintering crew at the German Antarctic Neumayer III station. During the nine months of operation, samples from the different plants, from the nutrition solution of the aeroponic planting system and from diverse surfaces within the three different compartments of the container were taken (future exploration greenhouse, service section, cold porch). Quantity as well as diversity of microorganisms was examined by cultivation. For identification, 16S rRNA gene sequencing was performed for the isolated prokaryotic organisms. In case of the plant samples, microbial quantities were in a range from 102 to 104 colony forming units per gram plant material. Compared to plants purchased from a German grocery, the produce hosted much more microorganisms than the EDEN ISS plants. The EDEN ISS plant samples contained mainly fungi and a few, most probably harmless bacteria. Most likely due to the used cultivation approach, Archaea were not found in the samples. The bioburden in the nutrition solutions increased constantly over time but never reached critical values. The surface samples revealed high differences in the microbial burden between the greenhouse part of the container and the service section and cold porch part. However, the numbers of organisms (bacteria and fungi) found in the planted greenhouse were still not critical. The microbial loaded surfaces showed strong temporal as well as spatial fluctuations. In samples of the nutrition solution and the surface, the amount of bacteria exceeded the amount of fungi by many times. The most abundant bacterial phyla were Firmicutes and Actinobacteria. These phyla include plant- and human-associated bacterial species. In general, there is a low risk of infection due to microbial contamination according to the results of this study. The metagenomic analysis of the EDENISS samples is ongoing.

Item URL in elib:https://elib.dlr.de/141311/
Document Type:Conference or Workshop Item (Speech)
Title:THE MICROBIOME OF A GREENHOUSE FOR GROUND DEMONSTRATION OF PLANT CULTIVATION TECHNOLOGIES IN SPACE
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iD
Rettberg, PetraRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; petra.rettberg (at) dlr.dehttps://orcid.org/0000-0003-4439-2395
Fahrion, JanaRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; jana.fahrion (at) dlr.deUNSPECIFIED
Zabel, PaulInstitute for Space Systems, German Aerospace Center (DLR), Bremen, Germany; Paul.Zabel (at) dlr.dehttps://orcid.org/0000-0001-7907-9230
Schubert, DanielInstitute for Space Systems, German Aerospace Center (DLR), Bremen, Germany; daniel.schubert (at) dlr.deUNSPECIFIED
Date:1 February 2021
Refereed publication:Yes
Open Access:Yes
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Status:Published
Keywords:EDEN ISS greenhouse, Antarctica, plant cultivation techniques, future space programs
Event Title:COSPAR 2021
Event Location:Sydney, Australia and Virtual Event (Hybrid)
Event Type:international Conference
Event Dates:28. Januar - 4. Februar 2021
Organizer:COSPAR - COMMITTEE ON SPACE RESEARCH
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - Project ISS LIFE 2.0
Location: Bremen , Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Institute of Space Systems > System Analysis Space Segment
Deposited By: Kopp, Kerstin
Deposited On:12 Mar 2021 10:45
Last Modified:14 Apr 2021 15:56

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