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GENE EXPRESSION COLLECTIVE DATA ANALYSIS FOR STUDYING THE EFFECTS OF HIGH-LET IONIZING RADIATION: A BIOINFORMATICS APPROACH

Michalettou, Theodora-Dafni and Diegeler, Sebastian and Kronenberg, Jessica and Nikitaki, Zacharenia and Hellweg, Christine E. and Georgakilas, Alexandros G. (2021) GENE EXPRESSION COLLECTIVE DATA ANALYSIS FOR STUDYING THE EFFECTS OF HIGH-LET IONIZING RADIATION: A BIOINFORMATICS APPROACH. COSPAR 2021, 28. Januar - 4. Februar 2021, Sydney, Australia and Virtual Event (Hybrid).

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Abstract

The use of high linear energy (LET) ionizing radiation (IR) is progressively being incorporated in radiation therapy (RT) due to its precise dose localization and high relative biological effectiveness. At the same time, these benefits of particle radiation become a high risk for astronauts in the case of inevitable long-term cosmic radiation exposure. Nonetheless, DNA Damage Response (DDR) activated via complex DNA damage on healthy tissue, occurring from such types of radiation, may be instrumental in the induction of various chronic and late effects. A method of approach in understanding the possible underlying mechanisms, is studying alterations in gene expression. To this end we identified Differentially Expressed Genes (DEGs) in IR-exposed healthy human tissue, utilizing microarray data available in public repositories. DEG analysis was conducted using R programming language. Consequently, through functional enrichment and biological network analysis, we identified biological pathways and processes implicated in DDR. By comparing low and high-LET radiation effects, our primary results indicate the induction of a differential biological response for high-LET, like an enhanced inflammatory response.In addition, patterns of DNA repair are substantially distinct compared to low-LET. Finally, we expanded our study in search of possible comorbidities for HZE particle exposure. Pathway enrichment analysis suggests the involvement of mechanisms, tightly correlated with neurodegenerative disorders like amyloid fibrils formation. Regarding blood tissue, platelet activation signaling was found, upholding the connection to cardiovascular disease. This holistic bioinformatics approach revealed cellular trends towards inflammation and degeneration which might be central to the development of late effects of high-LET radiation exposure. It can contribute to the identification of molecular targets for effective countermeasures.

Item URL in elib:https://elib.dlr.de/141300/
Document Type:Conference or Workshop Item (Poster)
Title:GENE EXPRESSION COLLECTIVE DATA ANALYSIS FOR STUDYING THE EFFECTS OF HIGH-LET IONIZING RADIATION: A BIOINFORMATICS APPROACH
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iD
Michalettou, Theodora-DafniNational Technical University of Athens (NTUA), Athens, Greece; daphne_lettos (at) yahoo.grUNSPECIFIED
Diegeler, SebastianRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; Sebastian.Diegeler (at) dlr.deUNSPECIFIED
Kronenberg, JessicaRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; jessica.kronenberg (at) dlr.deUNSPECIFIED
Nikitaki, ZachareniaNational Technical University of Athens (NTUA), Athens, Greece; znikitaki (at) mail.ntua.grUNSPECIFIED
Hellweg, Christine E.Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; Christine.Hellweg (at) dlr.dehttps://orcid.org/0000-0002-2223-3580
Georgakilas, Alexandros G.National Technical University of Athens (NTUA), Athens, Greece; alexg (at) mail.ntua.grUNSPECIFIED
Date:29 January 2021
Refereed publication:Yes
Open Access:Yes
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Status:Published
Keywords:high linear energy (LET), ionizing radiation (IR), DNA damage
Event Title:COSPAR 2021
Event Location:Sydney, Australia and Virtual Event (Hybrid)
Event Type:international Conference
Event Dates:28. Januar - 4. Februar 2021
Organizer:COSPAR - COMMITTEE ON SPACE RESEARCH
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - Project ISS LIFE 2.0
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:12 Mar 2021 10:40
Last Modified:14 Apr 2021 16:00

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