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Taxonomic and functional analyses of intact microbial communities thriving in extreme, astrobiology-relevant, anoxic sites

Bashir, Alexandra Kristin and Wink, Lisa and Duller, Stefanie and Schwendner, Petra and Cockell, Charles and Rettberg, Petra and Mahnert, Alexander and Beblo-Vranesevic, Kristina and Bohmeier, Maria and Rabbow, Elke and Gaboyer, Frederic and Westall, Frances and Walter, Nicolas and Cabezas, Patricia and Garcia-Descalzo, Laura and Gomez, Felipe and Malki, Mustapha and Amils, Ricardo and Ehrenfreund, Pascale and Monaghan, Euan and Vannier, Pauline and Marteinsson, Viggo and Erlacher, Armin and Tanski, George and Strauss, Jens and Bashir, Mina and Riedo, Andreas and Moissl-Eichinger, Christine (2021) Taxonomic and functional analyses of intact microbial communities thriving in extreme, astrobiology-relevant, anoxic sites. Microbiome, 9 (1), p. 50. BioMed Central. doi: 10.1186/s40168-020-00989-5. ISSN 2049-2618.

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Official URL: http://dx.doi.org/10.1186/s40168-020-00989-5

Abstract

Background: Extreme terrestrial, analogue environments are widely used models to study the limits of life and to infer habitability of extraterrestrial settings. In contrast to Earth’s ecosystems, potential extraterrestrial biotopes are usually characterized by a lack of oxygen. Methods: In the MASE project (Mars Analogues for Space Exploration), we selected representative anoxic analogue environments (permafrost, salt-mine, acidic lake and river, sulfur springs) for the comprehensive analysis of their microbial communities. We assessed the microbiome profile of intact cells by propidium monoazide-based amplicon and shotgun metagenome sequencing, supplemented with an extensive cultivation effort. Results: The information retrieved from microbiome analyses on the intact microbial community thriving in the MASE sites, together with the isolation of 31 model microorganisms and successful binning of 15 high-quality genomes allowed us to observe principle pathways, which pinpoint specific microbial functions in the MASE sites compared to moderate environments. The microorganisms were characterized by an impressive machinery to withstand physical and chemical pressures. All levels of our analyses revealed the strong and omnipresent dependency of the microbial communities on complex organic matter. Moreover, we identified an extremotolerant cosmopolitan group of 34 poly-extremophiles thriving in all sites. Conclusions: Our results reveal the presence of a core microbiome and microbial taxonomic similarities between saline and acidic anoxic environments. Our work further emphasizes the importance of the environmental, terrestrial parameters for the functionality of a microbial community, but also reveals a high proportion of living microorganisms in extreme environments with a high adaptation potential within habitability borders.

Item URL in elib:https://elib.dlr.de/141134/
Document Type:Article
Additional Information:This work was supported by European Community’s Seventh Framework Program (FP7/2007-2013) [grant agreement n° 607297].
Title:Taxonomic and functional analyses of intact microbial communities thriving in extreme, astrobiology-relevant, anoxic sites
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iD
Bashir, Alexandra KristinDiagnostic and Research Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, Graz, Austria and Department of Microbiology and Archaea Center, University of Regensburg, Regensburg, Germany.UNSPECIFIED
Wink, LisaDiagnostic and Research Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, Graz, Austria.UNSPECIFIED
Duller, StefanieDiagnostic and Research Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, Graz, Austria.UNSPECIFIED
Schwendner, PetraUK Center for Astrobiology, School of Physics and Astronomy, University of Edinburgh, Edinburgh, UK.UNSPECIFIED
Cockell, CharlesUK Center for Astrobiology, School of Physics and Astronomy, University of Edinburgh, Edinburgh, UK.UNSPECIFIED
Rettberg, PetraRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; petra.rettberg (at) dlr.dehttps://orcid.org/0000-0003-4439-2395
Mahnert, AlexanderDiagnostic and Research Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, Graz, Austria.UNSPECIFIED
Beblo-Vranesevic, KristinaRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; kristina.beblo (at) dlr.dehttps://orcid.org/0000-0002-4834-7121
Bohmeier, MariaInstitute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, GermanyUNSPECIFIED
Rabbow, ElkeRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; Elke.Rabbow (at) dlr.dehttps://orcid.org/0000-0002-9301-2021
Gaboyer, FredericCentre de Biophysique Moléculaire, Centre National de la Recherché Scientifique (CNRS), Orléans, France.UNSPECIFIED
Westall, FrancesCentre de Biophysique Moléculaire, Centre National de la Recherché Scientifique (CNRS), Orléans, France.UNSPECIFIED
Walter, NicolasEuropean Science Foundation (ESF), Strasbourg, France.UNSPECIFIED
Cabezas, PatriciaEuropean Science Foundation (ESF), Strasbourg, France.UNSPECIFIED
Garcia-Descalzo, LauraInstituto Nacional de Técnica Aeroespacial – Centro de Astrobiología (INTA-CAB), Madrid, Spain.UNSPECIFIED
Gomez, FelipeInstituto Nacional de Técnica Aeroespacial – Centro de Astrobiología (INTA-CAB), Madrid, Spain.UNSPECIFIED
Malki, MustaphaCentro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid (UAM), Madrid, Spain.UNSPECIFIED
Amils, RicardoCentro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid (UAM), Madrid, Spain.UNSPECIFIED
Ehrenfreund, PascaleLeiden Observatory, Universiteit Leiden, Leiden, The Netherlands.UNSPECIFIED
Monaghan, EuanLeiden Observatory, Universiteit Leiden, Leiden, The Netherlands.UNSPECIFIED
Vannier, PaulineMATIS, Reykjavík, Iceland.UNSPECIFIED
Marteinsson, ViggoMATIS, Reykjavík, Iceland and Faculty of Food Science and Nutrition, University of Iceland, Reykjavik, Iceland.UNSPECIFIED
Erlacher, ArminInstitute of Environmental Biotechnology, Graz University of Technology, Graz, Austria.UNSPECIFIED
Tanski, GeorgeAlfred Wegener Institute Helmholtz Centre for Polar and Marine Research, Periglacial Research Unit, Potsdam, Germany.UNSPECIFIED
Strauss, JensAlfred Wegener Institute Helmholtz Centre for Polar and Marine Research, Periglacial Research Unit, Potsdam, Germany.UNSPECIFIED
Bashir, MinaDivision of Endocrinology and Metabolism, Department of Internal Medicine, Graz, Austria.UNSPECIFIED
Riedo, AndreasSackler Laboratory for Astrophysics, Leiden Observatory, Leiden University, Leiden, The Netherlands.UNSPECIFIED
Moissl-Eichinger, ChristineDiagnostic and Research Institute of Hygiene, Microbiology and Environmental Medicine, Medical University of Graz, Graz, Austria and BioTechMed, Graz, Austria.https://orcid.org/0000-0001-6755-6263
Date:18 February 2021
Journal or Publication Title:Microbiome
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:9
DOI :10.1186/s40168-020-00989-5
Page Range:p. 50
Publisher:BioMed Central
ISSN:2049-2618
Status:Published
Keywords:Extreme environments, Microbiomes, Archaea, Bacteria, Propidium monoazide, Astrobiology, Spaceanalogue, Extremophiles, Extraterrestrial life, Metagenomics
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - Project ISS LIFE 2.0
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:12 Mar 2021 10:29
Last Modified:14 Apr 2021 14:26

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