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Changes in astrocytic behavior induced by altered gravity

Schunk, Sarah (2020) Changes in astrocytic behavior induced by altered gravity. Bachelor's, University of Bonn.

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After CNS injuries, neural regeneration and remyelination are inhibited, inter alia, by the formation of the glial scar. Although it prevents the widespread of damaged tissue, it is also preventing axon regrowth and therefore the healing of a CNS injury, leading to major and long-lasting or persistent restrictions. As soon as the mechanisms of this process are explored, possible therapeutic approaches to influence this process will be achievable. As astrocytes play an important role in the process of the formation of the glial scar, it is also essential to understand the mechanisms of the shift between their predominant non-reactive state and the reactive state following injury, as well as to find a way to reliably identify reactive astrocytes within the cell population. Previous studies have shown that astrocytes exposed to hypergravity in the physiological range (2 g) exhibited decreased cell spreading and migration rates in comparison to 1 g control samples. Furthermore, in contrast to astrocytes, neurons increase their neurite numbers as well as their neurite projection lengths under the influence of 2 g. Therefore, gravity might play an essential role in achieving possible therapeutic approaches to influence the formation of the glial scar. The aim of this thesis was to further investigate these promising results, focusing on the effects of hypergravity on key cellular parameters of primary murine astrocytes. These key parameters included apoptosis and necrosis, proliferation, reactivity, and morphology. Over the timespan of 120 h, for each parameter one assay was conducted, comparing 2 g samples with the 1 g control samples. Another important part of this thesis was to develop a measurable scoring approach to assess astrocyte reactivity, identifying if astrocytes are reactive, and subsequently in which state of reactivity they exist. This will build a base for further studies on astrocyte behavior under normal and altered gravity conditions. Overall, this study contributes to understanding the effects of hypergravity that is employed as a tool to specifically alter key astrocytic parameters, and thus influencing and possibly inhibiting the formation of a glial scar. Therefore, helping to find possible regeneration strategies, ideally, a regeneration method which induces a slower formation of a glial scar without the loss of the positive natural attributes could be developed.

Item URL in elib:https://elib.dlr.de/140210/
Document Type:Thesis (Bachelor's)
Additional Information:First Supervisor: Christian Liemersdorf Second Supervisor: Ruth Hemmersbach
Title:Changes in astrocytic behavior induced by altered gravity
AuthorsInstitution or Email of AuthorsAuthor's ORCID iDORCID Put Code
Schunk, SarahGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, Germany, and University of Bonn, Faculty of Mathematics and Natural Sciences, Biology, Bonn, GermanyUNSPECIFIEDUNSPECIFIED
Date:1 September 2020
Refereed publication:Yes
Open Access:No
Number of Pages:78
Keywords:Astrocytes, Hypergravity, CNS injuries, Glial scar
Institution:University of Bonn
Department:Faculty of Mathematics and Natural Sciences, Biology
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):Vorhaben: NeuroTox (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Gravitational Biology
Deposited By: Duwe, Helmut
Deposited On:11 Jan 2021 14:00
Last Modified:11 Jan 2021 14:00

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