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Resistance of the Archaeon Halococcus morrhuae and the Biofilm‐Forming Bacterium Halomonas muralis to Exposure to Low Earth Orbit for 534 Days

Leuko, Stefan and Stan‐Lotter, Helga and Lamers, Greta and Sjöström, Sebastian and Rabbow, Elke and Parpart, André and Rettberg, Petra (2020) Resistance of the Archaeon Halococcus morrhuae and the Biofilm‐Forming Bacterium Halomonas muralis to Exposure to Low Earth Orbit for 534 Days. In: Extremophiles as Astrobiological Models Wiley Online Library. pp. 221-236. doi: 10.1002/9781119593096.ch10. ISBN 9781119593096.

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Official URL: http://dx.doi.org/10.1002/9781119593096.ch10

Abstract

The halophilic archaeon Halococcus morrhuae and the biofilm‐forming bacterium Halomonas muralis were exposed to space conditions during the EXPOSE‐R2 mission. Evidence for both strains co‐existing on a mural in the castle Herberstein (Austria) has been found and here we tested the theory that the biofilm produced by Hlm. muralis may act as a protective layer for Hcc. morrhuae during exposure to extreme conditions. During Pre‐flight tests it was shown that Hcc. morrhuae is significantly more resistant to extreme conditions compared to Hlm. muralis. A mixture of both strains proved advantageous only for the survival of Hcc. morrhuae. Hcc. morrhuae was exposed independently and in combination with Hlm. muralis to space conditions outside of the International Space Station (ISS) for 534 days. Survival of Hcc. morrhuae was investigated by most probable number test and colony‐forming unit assay; damage inflicted to genomic DNA was evaluated by random amplified polymorphic DNA‐PCR and the integrity of the 16S rRNA by real‐time PCR. Exposure to extreme conditions on Earth as well as in outer space had a strong detrimental effect on the survival and genomic stability of Hcc. morrhuae; however, we were able to re‐cultivate Hcc. morrhuae from samples exposed outside of the ISS.

Item URL in elib:https://elib.dlr.de/140094/
Document Type:Book Section
Additional Information:Chapter 10
Title:Resistance of the Archaeon Halococcus morrhuae and the Biofilm‐Forming Bacterium Halomonas muralis to Exposure to Low Earth Orbit for 534 Days
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iDORCID Put Code
Leuko, StefanUNSPECIFIEDhttps://orcid.org/0000-0002-0423-0896UNSPECIFIED
Stan‐Lotter, HelgaUniversity of Salzburg, Department of Biosciences, Salzburg, AustriaUNSPECIFIEDUNSPECIFIED
Lamers, GretaETH Zürich, Department of Health Sciences and Technology, Schwerzenbach, SwitzerlandUNSPECIFIEDUNSPECIFIED
Sjöström, SebastianETH Zürich, Department of Chemistry and Applied Biosciences, Zürich, SwitzerlandUNSPECIFIEDUNSPECIFIED
Rabbow, ElkeUNSPECIFIEDhttps://orcid.org/0000-0002-9301-2021UNSPECIFIED
Parpart, AndréRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, GermanyUNSPECIFIEDUNSPECIFIED
Rettberg, PetraUNSPECIFIEDhttps://orcid.org/0000-0003-4439-2395UNSPECIFIED
Date:4 December 2020
Journal or Publication Title:Extremophiles as Astrobiological Models
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
DOI:10.1002/9781119593096.ch10
Page Range:pp. 221-236
Editors:
EditorsEmailEditor's ORCID iDORCID Put Code
Seckbach, JosephUNSPECIFIEDUNSPECIFIEDUNSPECIFIED
Stan‐Lotter, HelgaUniversity of Salzburg, Department of Biosciences, Salzburg, AustriaUNSPECIFIEDUNSPECIFIED
Publisher:Wiley Online Library
ISBN:9781119593096
Status:Published
Keywords:Halophiles, biofilm, prolonged outer space exposure, BOSS, ISS, EXPOSE‐R2
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:12 Jan 2021 10:47
Last Modified:12 Jan 2021 10:47

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