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Systems View of Deconditioning During Spaceflight Simulation in the PlanHab Project: The Departure of Urine 1 H-NMR Metabolomes From Healthy State in Young Males Subjected to Bedrest Inactivity and Hypoxia

Šket, Robert and Deutsch, Leon and Prevoršek, Zala and Mekjavic, Igor B. and Plavec, Janez and Rittweger, Jörn and Debevec, Tadej and Eiken, Ola and Stres, Blaz (2020) Systems View of Deconditioning During Spaceflight Simulation in the PlanHab Project: The Departure of Urine 1 H-NMR Metabolomes From Healthy State in Young Males Subjected to Bedrest Inactivity and Hypoxia. Frontiers in Physiology, 11, p. 532271. Frontiers Media S.A. doi: 10.3389/fphys.2020.532271. ISSN 1664-042X.

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Official URL: https://www.frontiersin.org/articles/10.3389/fphys.2020.532271/full

Abstract

We explored the metabolic makeup of urine in prescreened healthy male participants within the PlanHab experiment. The run-in (5 day) and the following three 21-day interventions [normoxic bedrest (NBR), hypoxic bedrest (HBR), and hypoxic ambulation (HAmb)] were executed in a crossover manner within a controlled laboratory setup (medical oversight, fluid and dietary intakes, microbial bioburden, circadian rhythm, and oxygen level). The inspired O₂ (FᵢO₂) fraction next to inspired O₂ (PᵢO₂) partial pressure were 0.209 and 133.1 ± 0.3 mmHg for the NBR variant in contrast to 0.141 ± 0.004 and 90.0 ± 0.4 mmHg (approx. 4,000 m of simulated altitude) for HBR and HAmb interventions, respectively. ¹H-NMR metabolomes were processed using standard quantitative approaches. A consensus of ensemble of multivariate analyses showed that the metabolic makeup at the start of the experiment and at HAmb endpoint differed significantly from the NBR and HBR endpoints. Inactivity alone or combined with hypoxia resulted in a significant reduction of metabolic diversity and increasing number of affected metabolic pathways. Sliding window analysis (3 + 1) unraveled that metabolic changes in the NBR lagged behind those observed in the HBR. These results show that the negative effects of cessation of activity on systemic metabolism are further aggravated by additional hypoxia. The PlanHab HAmb variant that enabled ambulation, maintained vertical posture, and controlled but limited activity levels apparently prevented the development of negative physiological symptoms such as insulin resistance, low-level systemic inflammation, constipation, and depression. This indicates that exercise apparently prevented the negative spiral between the host's metabolism, intestinal environment, microbiome physiology, and proinflammatory immune activities in the host.

Item URL in elib:https://elib.dlr.de/140044/
Document Type:Article
Title:Systems View of Deconditioning During Spaceflight Simulation in the PlanHab Project: The Departure of Urine 1 H-NMR Metabolomes From Healthy State in Young Males Subjected to Bedrest Inactivity and Hypoxia
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iD
Šket, RobertDepartment of Animal Science, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia,UNSPECIFIED
Deutsch, LeonDepartment of Animal Science, Biotechnical Faculty, University of Ljubljana, Ljubljana, Slovenia,UNSPECIFIED
Prevoršek, ZalaDepartment of Animal Science, Biotechnical Faculty, University of Ljubljana, Ljubljana, SloveniaUNSPECIFIED
Mekjavic, Igor B.Jozef Stefan InstituteUNSPECIFIED
Plavec, JanezNational Institute of Chemistry,NMR Center, Ljubljana, SloveniaUNSPECIFIED
Rittweger, JörnJoern.Rittweger (at) dlr.dehttps://orcid.org/0000-0002-2223-8963
Debevec, TadejDepartment of Automation, Biocybernetics and Robotics, Jozef Stefan Institute, Ljubljana, SloveniaUNSPECIFIED
Eiken, OlaDepartment of Environmental Physiology, Swedish Aerospace Physiology Centre, Royal Institute of Technology, Stockholm, SwedenUNSPECIFIED
Stres, BlazDepartment of Animal Science, Biotechnical Faculty, University of Ljubljana, Ljubljana, SloveniaUNSPECIFIED
Date:7 December 2020
Journal or Publication Title:Frontiers in Physiology
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:11
DOI :10.3389/fphys.2020.532271
Page Range:p. 532271
Publisher:Frontiers Media S.A
Series Name:Gastrointestinal Sciences
ISSN:1664-042X
Status:Published
Keywords:urine, metabolome, NMR, inactivity, interplanetary travel, medicine, deconditioning, inflammation
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - Vorhaben Systemphysiologie (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Muscle and Bone Metabolism
Deposited By: Arndt, Carina
Deposited On:06 Jan 2021 14:20
Last Modified:06 Jan 2021 14:20

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