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Enhanced Blood Supply Through Lower Body Negative Pressure During Slow-Paced, High Load Leg Press Exercise Alters the Response of Muscle AMPK and Circulating Angiogenic Factors

Parganlija, Dajana und Gehlert, Sebastian und Herrera, Frankyn und Rittweger, Jörn und Bloch, Wilhelm und Zange, Jochen (2020) Enhanced Blood Supply Through Lower Body Negative Pressure During Slow-Paced, High Load Leg Press Exercise Alters the Response of Muscle AMPK and Circulating Angiogenic Factors. Frontiers in Physiology, 11, Seite 781. Frontiers Media S.A. doi: 10.3389/fphys.2020.00781. ISSN 1664-042X.

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Offizielle URL: https://www.frontiersin.org/articles/10.3389/fphys.2020.00781/full

Kurzfassung

Lower body negative pressure (LBNP) is an established method of simulating the gravitational effects of orthostasis on the cardiovascular system during space flight or at supine body position on Earth. We hypothesized that LBNP added onto leg press exercise would promote leg muscle perfusion, stimulate oxygen consumption, and modify acute molecular responses. Eighteen subjects performed fifteen slow-paced concentric (4 s) and eccentric contractions (4 s) without or with 40 mmHg LBNP. Force corresponding to 6% of the one-repetition maximum (1-RM) at knee flexion gradually increased to 60% 1-RM within the first half of the range of motion, thereafter remaining constant. AMPK and P-AMPK protein expression was determined in biopsies of vastus lateralis. Venous blood samples were used to measure angiogenic factors. Physiological responses to LBNP included an elevated EMG amplitude, higher heart rate and doubling of the cardiac output compared to control (p < 0.001). Muscle total hemoglobin was increased by around 20 mmol/l vs. control (p < 0.001), accompanied by decreasing tissue oxygen saturation and elevated oxygen uptake (p < 0.05). MMP-2 levels were reduced, and the ratio of P-AMPK to AMPK elevated after exercise with LBNP (p < 0.05). MMP-9 similarly increased in both groups, whereas endostatin was only elevated in the control group (p < 0.05). Our results indicate facilitated peripheral blood supply and higher oxygen exploitation leading to activation of the energy sensor AMPK and differential regulation of angiogenic factors involved in muscle tissue remodeling and capillary growth. Simulating orthostasis with LBNP might promote beneficial structural adaptations of skeletal muscles during resistance exercise and contribute to future exercise countermeasures achieving increased muscle strength and endurance during space flight.

elib-URL des Eintrags:https://elib.dlr.de/135973/
Dokumentart:Zeitschriftenbeitrag
Titel:Enhanced Blood Supply Through Lower Body Negative Pressure During Slow-Paced, High Load Leg Press Exercise Alters the Response of Muscle AMPK and Circulating Angiogenic Factors
Autoren:
AutorenInstitution oder E-Mail-AdresseAutoren-ORCID-iDORCID Put Code
Parganlija, DajanaSpace Physiology Department, Institute of AerospaceNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Gehlert, SebastianDepartment of Molecular and Cellular Sport Medicine, German Sport University CologneNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Herrera, FrankynGerman Aerospace Center, Institute of Aerospace Medicine and Space Physiology, CologneNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Rittweger, JörnJoern.Rittweger (at) dlr.dehttps://orcid.org/0000-0002-2223-8963NICHT SPEZIFIZIERT
Bloch, WilhelmGerman Sport University Colognehttps://orcid.org/0000-0003-1786-8853NICHT SPEZIFIZIERT
Zange, JochenJochen.Zange (at) dlr.dehttps://orcid.org/0000-0003-1822-0952133724697
Datum:30 Juli 2020
Erschienen in:Frontiers in Physiology
Referierte Publikation:Ja
Open Access:Ja
Gold Open Access:Ja
In SCOPUS:Ja
In ISI Web of Science:Ja
Band:11
DOI:10.3389/fphys.2020.00781
Seitenbereich:Seite 781
Verlag:Frontiers Media S.A
ISSN:1664-042X
Status:veröffentlicht
Stichwörter:resistance exercise, robotically controlled leg press, lower body negative pressure, muscle perfusion, physiological responses, AMPK, MMP, endostatin
HGF - Forschungsbereich:Luftfahrt, Raumfahrt und Verkehr
HGF - Programm:Raumfahrt
HGF - Programmthema:Forschung unter Weltraumbedingungen
DLR - Schwerpunkt:Raumfahrt
DLR - Forschungsgebiet:R FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):R - Vorhaben Systemphysiologie (alt)
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Muskel- und Knochenstoffwechsel
Hinterlegt von: Arndt, Carina
Hinterlegt am:08 Sep 2020 13:23
Letzte Änderung:30 Okt 2023 13:44

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