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Enhanced Blood Supply Through Lower Body Negative Pressure During Slow-Paced, High Load Leg Press Exercise Alters the Response of Muscle AMPK and Circulating Angiogenic Factors

Parganlija, Dajana and Gehlert, Sebastian and Herrera, Frankyn and Rittweger, Jörn and Bloch, Wilhelm and Zange, Jochen (2020) Enhanced Blood Supply Through Lower Body Negative Pressure During Slow-Paced, High Load Leg Press Exercise Alters the Response of Muscle AMPK and Circulating Angiogenic Factors. Frontiers in Physiology, 11, p. 781. Frontiers Media S.A. doi: 10.3389/fphys.2020.00781. ISSN 1664-042X.

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Official URL: https://www.frontiersin.org/articles/10.3389/fphys.2020.00781/full

Abstract

Lower body negative pressure (LBNP) is an established method of simulating the gravitational effects of orthostasis on the cardiovascular system during space flight or at supine body position on Earth. We hypothesized that LBNP added onto leg press exercise would promote leg muscle perfusion, stimulate oxygen consumption, and modify acute molecular responses. Eighteen subjects performed fifteen slow-paced concentric (4 s) and eccentric contractions (4 s) without or with 40 mmHg LBNP. Force corresponding to 6% of the one-repetition maximum (1-RM) at knee flexion gradually increased to 60% 1-RM within the first half of the range of motion, thereafter remaining constant. AMPK and P-AMPK protein expression was determined in biopsies of vastus lateralis. Venous blood samples were used to measure angiogenic factors. Physiological responses to LBNP included an elevated EMG amplitude, higher heart rate and doubling of the cardiac output compared to control (p < 0.001). Muscle total hemoglobin was increased by around 20 mmol/l vs. control (p < 0.001), accompanied by decreasing tissue oxygen saturation and elevated oxygen uptake (p < 0.05). MMP-2 levels were reduced, and the ratio of P-AMPK to AMPK elevated after exercise with LBNP (p < 0.05). MMP-9 similarly increased in both groups, whereas endostatin was only elevated in the control group (p < 0.05). Our results indicate facilitated peripheral blood supply and higher oxygen exploitation leading to activation of the energy sensor AMPK and differential regulation of angiogenic factors involved in muscle tissue remodeling and capillary growth. Simulating orthostasis with LBNP might promote beneficial structural adaptations of skeletal muscles during resistance exercise and contribute to future exercise countermeasures achieving increased muscle strength and endurance during space flight.

Item URL in elib:https://elib.dlr.de/135973/
Document Type:Article
Title:Enhanced Blood Supply Through Lower Body Negative Pressure During Slow-Paced, High Load Leg Press Exercise Alters the Response of Muscle AMPK and Circulating Angiogenic Factors
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iD
Parganlija, DajanaSpace Physiology Department, Institute of AerospaceUNSPECIFIED
Gehlert, SebastianDepartment of Molecular and Cellular Sport Medicine, German Sport University CologneUNSPECIFIED
Herrera, FrankynGerman Aerospace Center, Institute of Aerospace Medicine and Space Physiology, CologneUNSPECIFIED
Rittweger, JörnJoern.Rittweger (at) dlr.dehttps://orcid.org/0000-0002-2223-8963
Bloch, WilhelmGerman Sport University Colognehttps://orcid.org/0000-0003-1786-8853
Zange, JochenJochen.Zange (at) dlr.dehttps://orcid.org/0000-0003-1822-0952
Date:30 July 2020
Journal or Publication Title:Frontiers in Physiology
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:11
DOI :10.3389/fphys.2020.00781
Page Range:p. 781
Publisher:Frontiers Media S.A
ISSN:1664-042X
Status:Published
Keywords:resistance exercise, robotically controlled leg press, lower body negative pressure, muscle perfusion, physiological responses, AMPK, MMP, endostatin
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - Vorhaben Systemphysiologie (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Muscle and Bone Metabolism
Deposited By: Arndt, Carina
Deposited On:08 Sep 2020 13:23
Last Modified:30 Sep 2020 10:17

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