elib
DLR-Header
DLR-Logo -> http://www.dlr.de
DLR Portal Home | Impressum | Datenschutz | Kontakt | English
Schriftgröße: [-] Text [+]

The MAPHEUS module CellFix for studying the influence of altered gravity on the physiology of single cells

Liemersdorf, Christian und Lichterfeld, Yannick und Hemmersbach, Ruth und Hauslage, Jens (2020) The MAPHEUS module CellFix for studying the influence of altered gravity on the physiology of single cells. Review of Scientific Instruments, 91 (1), 014101. American Institute of Physics (AIP). doi: 10.1063/1.5121504. ISSN 0034-6748.

Dieses Archiv kann nicht den Volltext zur Verfügung stellen.

Offizielle URL: https://doi.org/10.1063/1.5121504

Kurzfassung

Gravity is the only constant stimulus during the evolution of life. To investigate the impact of the absence of gravity on living systems, their molecular and morphological status has to be studied under microgravity conditions. The experiment unit CellFix was developed in order to provide the possibility of exposure and chemical fixation of small biological systems, such as neurons, stem cells, small animals, yeast cultures, plants, etc., at dedicated time points during a sounding rocket flight. The current version of CellFix consists of two culture bags containing cell cultures in a temperature-controlled pressure vessel. The biosystems in the culture bags can be fixed by pumping the fixative [e.g., paraformaldehyde (PFA), methanol, RNAlater, or others] from a connected bag into the cell suspension. The mechatronic basis of the experiment unit is constructed from compartments of the shelf parts. Open source microcontroller systems (Arduino) or gear pumps, accumulators, etc., from the model making sector are affordable and reliable components to build up an experiment on an unmanned space mission such as a sounding rocket flight. Also, new technologies such as fused deposition modeling were used to construct structures and brackets, which were tested successfully in environmental tests and real space flights (MAPHEUS 7 and 8 sounding rocket missions). In combination with the possibility to handle the experiment as a late access insert in a standardized rocket compartment, CellFix provides a multi-usable experiment unit for performing life science experiments in space.

elib-URL des Eintrags:https://elib.dlr.de/135458/
Dokumentart:Zeitschriftenbeitrag
Titel:The MAPHEUS module CellFix for studying the influence of altered gravity on the physiology of single cells
Autoren:
AutorenInstitution oder E-Mail-AdresseAutoren-ORCID-iDORCID Put Code
Liemersdorf, Christiangerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0001-8407-5226NICHT SPEZIFIZIERT
Lichterfeld, Yannickgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0001-8755-9920NICHT SPEZIFIZIERT
Hemmersbach, Ruthgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0001-5308-6715NICHT SPEZIFIZIERT
Hauslage, Jensgerman aerospace center (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0003-2184-7000NICHT SPEZIFIZIERT
Datum:2 Januar 2020
Erschienen in:Review of Scientific Instruments
Referierte Publikation:Ja
Open Access:Nein
Gold Open Access:Nein
In SCOPUS:Ja
In ISI Web of Science:Ja
Band:91
DOI:10.1063/1.5121504
Seitenbereich:014101
Verlag:American Institute of Physics (AIP)
ISSN:0034-6748
Status:veröffentlicht
Stichwörter:MAPHEUS module, CellFix, Altered gravity, Cells
HGF - Forschungsbereich:Luftfahrt, Raumfahrt und Verkehr
HGF - Programm:Raumfahrt
HGF - Programmthema:Forschung unter Weltraumbedingungen
DLR - Schwerpunkt:Raumfahrt
DLR - Forschungsgebiet:R FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):R - Vorhaben Biowissenschaftliche Exp.-vorbereitung (alt), R - Vorhaben Biowissenschaftliche Nutzerunterstützung (alt)
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Gravitationsbiologie
Hinterlegt von: Duwe, Helmut
Hinterlegt am:06 Aug 2020 09:45
Letzte Änderung:24 Okt 2023 11:11

Nur für Mitarbeiter des Archivs: Kontrollseite des Eintrags

Blättern
Suchen
Hilfe & Kontakt
Informationen
electronic library verwendet EPrints 3.3.12
Gestaltung Webseite und Datenbank: Copyright © Deutsches Zentrum für Luft- und Raumfahrt (DLR). Alle Rechte vorbehalten.