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The MAPHEUS module CellFix for studying the influence of altered gravity on the physiology of single cells

Liemersdorf, Christian and Lichterfeld, Yannick and Hemmersbach, Ruth and Hauslage, Jens (2020) The MAPHEUS module CellFix for studying the influence of altered gravity on the physiology of single cells. Review of Scientific Instruments, 91 (1), 014101. American Institute of Physics (AIP). doi: 10.1063/1.5121504. ISSN 0034-6748.

Full text not available from this repository.

Official URL: https://doi.org/10.1063/1.5121504

Abstract

Gravity is the only constant stimulus during the evolution of life. To investigate the impact of the absence of gravity on living systems, their molecular and morphological status has to be studied under microgravity conditions. The experiment unit CellFix was developed in order to provide the possibility of exposure and chemical fixation of small biological systems, such as neurons, stem cells, small animals, yeast cultures, plants, etc., at dedicated time points during a sounding rocket flight. The current version of CellFix consists of two culture bags containing cell cultures in a temperature-controlled pressure vessel. The biosystems in the culture bags can be fixed by pumping the fixative [e.g., paraformaldehyde (PFA), methanol, RNAlater, or others] from a connected bag into the cell suspension. The mechatronic basis of the experiment unit is constructed from compartments of the shelf parts. Open source microcontroller systems (Arduino) or gear pumps, accumulators, etc., from the model making sector are affordable and reliable components to build up an experiment on an unmanned space mission such as a sounding rocket flight. Also, new technologies such as fused deposition modeling were used to construct structures and brackets, which were tested successfully in environmental tests and real space flights (MAPHEUS 7 and 8 sounding rocket missions). In combination with the possibility to handle the experiment as a late access insert in a standardized rocket compartment, CellFix provides a multi-usable experiment unit for performing life science experiments in space.

Item URL in elib:https://elib.dlr.de/135458/
Document Type:Article
Title:The MAPHEUS module CellFix for studying the influence of altered gravity on the physiology of single cells
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iDORCID Put Code
Liemersdorf, Christiangerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0001-8407-5226UNSPECIFIED
Lichterfeld, Yannickgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0001-8755-9920UNSPECIFIED
Hemmersbach, Ruthgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0001-5308-6715UNSPECIFIED
Hauslage, Jensgerman aerospace center (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0003-2184-7000UNSPECIFIED
Date:2 January 2020
Journal or Publication Title:Review of Scientific Instruments
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:91
DOI:10.1063/1.5121504
Page Range:014101
Publisher:American Institute of Physics (AIP)
ISSN:0034-6748
Status:Published
Keywords:MAPHEUS module, CellFix, Altered gravity, Cells
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Exp.-vorbereitung (old), R - Vorhaben Biowissenschaftliche Nutzerunterstützung (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Gravitational Biology
Deposited By: Duwe, Helmut
Deposited On:06 Aug 2020 09:45
Last Modified:24 Oct 2023 11:11

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