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Backward translation to the cellular level. Studies under artificial gravity conditions

Hemmersbach, Ruth and Liemersdorf, Christian and Brungs, Sonja and Waßer, Kai and Hauslage, Jens and Jordan, Jens (2019) Backward translation to the cellular level. Studies under artificial gravity conditions. 22nd IAA Humans in Space Symposium (HIS), 11.–14. Nov. 2019, Dubai, UAE.

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Abstract

Suitable platforms providing well defined and reproducible artificial gravity conditions, particularly simulated microgravity and hypergravity, have a pivotal role in preparing space-based experiments. Clinostats and Random Positioning Machines (RPM) are commonly applied in gravitational cell biology to simulate microgravity conditions. To exclude potential non-gravitational cellular responses to both methodologies, we applied the highly sensitive reporter organism, the dinoflagellate Pyrocystis noctiluca. The organism responds to shear stress with detectable bioluminescence emission. Pyrocystis noctiluca was exposed to different microgravity simulations while varying operational modes. With cells in a RPM rotating around two axes with random velocities and directions, we observed significantly greater mechanical stress compared with clinostat experiments applying constant rotation around one axis. We conclude that in contrast to RPM, one axis clinorotation induces substantially less non-gravitational responses through shear forces. Therefore, we apply clinostats as our preferred and validated method for the simulation of microgravity in ongoing cellular experiments. Altered gravity conditions elicit physiological changes in the human body. Translation from humans to cells will help identifying fundamental gravity-related mechanisms and targets for preventative measures. Our cell models include stem cells, neurons, glia, cardiac muscle, and immune cells. Case studies applying a broad range of ground-based artificial gravity facilities, which are available in our research platform :envihab, will be presented.

Item URL in elib:https://elib.dlr.de/134774/
Document Type:Conference or Workshop Item (Speech)
Title:Backward translation to the cellular level. Studies under artificial gravity conditions
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iD
Hemmersbach, RuthGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, Germanyhttps://orcid.org/0000-0001-5308-6715
Liemersdorf, Christiangerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0001-8407-5226
Brungs, Sonjagerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0002-1277-9942
Waßer, KaiGerman Aerospace Centre (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, GermanyUNSPECIFIED
Hauslage, Jensgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0003-2184-7000
Jordan, Jensgerman aerospace centre (dlr), institute of aerospace medicine, cologne, germanyhttps://orcid.org/0000-0003-4518-0706
Date:11 November 2019
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Status:Published
Keywords:Microgravity, Artificial gravity, Clinostats, Random Positioning Machines (RPM), Pyrocystis noctiluca
Event Title:22nd IAA Humans in Space Symposium (HIS)
Event Location:Dubai, UAE
Event Type:international Conference
Event Dates:11.–14. Nov. 2019
Organizer:International Academy of Astronautics (IAA)
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Exp.-vorbereitung (old), R - Vorhaben Biowissenschaftliche Nutzerunterstützung (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Gravitational Biology
Institute of Aerospace Medicine > Leitungsbereich ME
Deposited By: Duwe, Helmut
Deposited On:13 May 2020 15:31
Last Modified:13 May 2020 15:31

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