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Impact of Simulated Martian Conditions on (Facultatively) Anaerobic Bacterial Strains from Different Mars Analogue Sites

Beblo-Vranesevic, Kristina and Bohmeier, Maria and Schleumer, Sven and Rabbow, Elke and Perras, Alexandra K. and Moissl-Eichinger, Christine and Schwendner, Petra and Cockell, Charles S. and Vannier, Pauline and Marteinsson, Viggo T. and Monaghan, Euan P. and Riedo, Andreas and Ehrenfreund, Pascale and Garcia-Descalzo, Laura and Gómez, Felipe and Malki, Moustafa and Amils, Ricardo and Gaboyer, Frédéric and Hickman-Lewis, Keyron and Westall, Frances and Cabezas, Patricia and Walter, Nicolas and Rettberg, Petra (2020) Impact of Simulated Martian Conditions on (Facultatively) Anaerobic Bacterial Strains from Different Mars Analogue Sites. Current Issues in Molecular Biology, 38, pp. 103-122. Caister Academic Press. doi: 10.21775/cimb.038.103. ISSN 1467-3037.

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Official URL: http://dx.doi.org/10.21775/cimb.038.103

Abstract

Five bacterial (facultatively) anaerobic strains, namely Buttiauxella sp. MASE-IM-9, Clostridium sp. MASE-IM-4, Halanaerobium sp. MASE-BB-1, Trichococcus sp. MASE-IM-5, and Yersinia intermedia MASE-LG-1 isolated from different extreme natural environments were subjected to Mars relevant environmental stress factors in the laboratory under controlled conditions. These stress factors encompassed low water activity, oxidizing compounds, and ionizing radiation. Stress tests were performed under permanently anoxic conditions. The survival rate after addition of sodium perchlorate (Na-perchlorate) was found to be species-specific. The inter-comparison of the five microorganisms revealed that Clostridium sp. MASE-IM-4 was the most sensitive strain (D₁₀-value (15 min, NaClO₄) = 0.6 M). The most tolerant microorganism was Trichococcus sp. MASE-IM-5 with a calculated D₁₀-value (15 min, NaClO₄) of 1.9 M. Cultivation in the presence of Na-perchlorate in Martian relevant concentrations up to 1 wt% led to the observation of chains of cells in all strains. Exposure to Na-perchlorate led to a lowering of the survival rate after desiccation. Consecutive exposure to desiccating conditions and ionizing radiation led to additive effects. Moreover, in a desiccated state, an enhanced radiation tolerance could be observed for the strains Clostridium sp. MASE-IM-4 and Trichococcus sp. MASE-IM-5. These data show that anaerobic microorganisms from Mars analogue environments can resist a variety of Martian-simulated stresses either individually or in combination. However, responses were species-specific and some Mars-simulated extremes killed certain organisms. Thus, although Martian stresses would be expected to act differentially on microorganisms, none of the expected extremes tested here and found on Mars prevent the growth of anaerobic microorganisms.

Item URL in elib:https://elib.dlr.de/134675/
Document Type:Article
Title:Impact of Simulated Martian Conditions on (Facultatively) Anaerobic Bacterial Strains from Different Mars Analogue Sites
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iD
Beblo-Vranesevic, KristinaRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; kristina.beblo (at) dlr.dehttps://orcid.org/0000-0002-4834-7121
Bohmeier, MariaRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; Maria.Bohmeier (at) dlr.deUNSPECIFIED
Schleumer, SvenRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany and Hochschule Niederrhein, Wirtschaftingenieurswesen, Krefeld, Germany.UNSPECIFIED
Rabbow, ElkeRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; Elke.Rabbow (at) dlr.dehttps://orcid.org/0000-0002-9301-2021
Perras, Alexandra K.Department of Internal Medicine, Medical University of Graz, Graz, Austria and Department of Microbiology and Archaea, University of Regensburg, Regensburg, GermanyUNSPECIFIED
Moissl-Eichinger, ChristineDepartment of Internal Medicine, Medical University of Graz, Graz, Austria and BioTechMed Graz, Graz, AustriaUNSPECIFIED
Schwendner, PetraUK Center for Astrobiology, School of Physics and Astronomy, University of Edinburgh, Edinburgh, UK and Department of Plant Pathology, Space Life Sciences Laboratory, University of Florida, FL, USAUNSPECIFIED
Cockell, Charles S.UK Center for Astrobiology, School of Physics and Astronomy, University of Edinburgh, Edinburgh, UKUNSPECIFIED
Vannier, PaulineMATIS - Prokaria, Reykjavík, IcelandUNSPECIFIED
Marteinsson, Viggo T.MATIS - Prokaria, Reykjavík, Iceland and Faculty of Food Science and Nutrition, University of Iceland, Reykjavík, IcelandUNSPECIFIED
Monaghan, Euan P.Leiden Observatory, Universiteit Leiden, Leiden, NetherlandsUNSPECIFIED
Riedo, AndreasLeiden Observatory, Universiteit Leiden, Leiden, NetherlandUNSPECIFIED
Ehrenfreund, PascaleLeiden Observatory, Universiteit Leiden, Leiden, Netherland and Space Policy Institute, George Washington University, Washington DC, USAUNSPECIFIED
Garcia-Descalzo, LauraInstituto Nacional de Técnica Aeroespacial - Centro de Astrobiología (INTA-CAB), Madrid, SpainUNSPECIFIED
Gómez, FelipeInstituto Nacional de Técnica Aeroespacial - Centro de Astrobiología (INTA-CAB), Madrid, SpainUNSPECIFIED
Malki, MoustafaCentro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid (UAM), Madrid, SpainUNSPECIFIED
Amils, RicardoCentro de Biología Molecular Severo Ochoa, Universidad Autónoma de Madrid (UAM), Madrid, SpainUNSPECIFIED
Gaboyer, FrédéricCentre de Biophysique Moléculaire, Centre National de la Recherche Scientifique (CNRS), Orléans, FranceUNSPECIFIED
Hickman-Lewis, KeyronCentre de Biophysique Moléculaire, Centre National de la Recherche Scientifique (CNRS), Orléans, FranceUNSPECIFIED
Westall, FrancesCentre de Biophysique Moléculaire, Centre National de la Recherche Scientifique (CNRS), Orléans, FranceUNSPECIFIED
Cabezas, PatriciaEuropean Science Foundation (ESF), Strasbourg, FranceUNSPECIFIED
Walter, NicolasEuropean Science Foundation (ESF), Strasbourg, FranceUNSPECIFIED
Rettberg, PetraRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), 51147 Cologne, Germany; Petra.Rettberg (at) dlr.dehttps://orcid.org/0000-0003-4439-2395
Date:April 2020
Journal or Publication Title:Current Issues in Molecular Biology
Refereed publication:Yes
Open Access:Yes
Gold Open Access:No
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:38
DOI :10.21775/cimb.038.103
Page Range:pp. 103-122
Publisher:Caister Academic Press
ISSN:1467-3037
Status:Published
Keywords:MASE, Mars, simulated Martian conditions, anearobic bacterial strains
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:22 Apr 2020 13:35
Last Modified:19 Nov 2021 20:40

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