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Neuronal Regeneration Induced by Exposure to Altered Gravity

Liemersdorf, Christian and Lichterfeld, Yannick and Frett, Timo and Jordan, Jens and Hemmersbach, Ruth (2019) Neuronal Regeneration Induced by Exposure to Altered Gravity. Annual Meeting of the Bonn Forum Biomedizin (BFB), 7. - 8. März 2019, Hennef / Bonn.

Full text not available from this repository.

Official URL: https://www.bfb.uni-bonn.de/

Abstract

Disturbed neuronal connectivity is the ultimate cause of disability in individuals with neurological disease including spinal cord injury, head trauma, and stroke. Functional neurological recovery is limited through an unfavorable balance between neuronal regrowth and glia scar formation. Neuronal growth requires dynamic cytoskeletal protein rearrangements. Because hypergravity stabilizes microtubules while de-stabilizing actin filaments, we hypothesized that experimental hypergravity would shift the balance between neuronal and astroglial growth in vitro. We exposed murine primary hippocampal neurons during different developmental stages as well as primary murine astrocytes to 2g using the DLR hypergravity platform. This platform unlike commercial laboratory centrifuges models physiological hypergravity and allows for cell cultivation and live-cell imaging. We assessed neuritogenesis, neuronal polarization and maturation processes including synaptogenesis and synaptic integration in mature neural networks. Moreover, we studied the morphology and migration of primary astrocytes to shed light on their role during glial scar formation at neural lesion sites. Exposure of hippocampal neurons to 24h of 2g hypergravity increased neurite number by 30% and neurite projection length by 20% compared to 1g. At later developmental stages, mature synaptic contacts were formed under hypergravity conditions. In contrast, astrocytes showed decreases in cell spreading and lamellipodial protrusions as well as a diminished migratory rate with hypergravity. We conclude that experimental hypergravity ameliorates neuronal cell growth and synaptic contacts while halting astrocyte spreading and migration in vivo. Given the importance of this balance for neuronal regeneration in human neurological disease, we will now study the underlying mechanisms in more detail using, both, DLR hypergravity and clinostat-induced hypogravity platforms.

Item URL in elib:https://elib.dlr.de/134603/
Document Type:Conference or Workshop Item (Speech)
Title:Neuronal Regeneration Induced by Exposure to Altered Gravity
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iD
Liemersdorf, Christiangravitational biology, institute of aerospace medicine, german aerospace centre (dlr), cologne, germanyhttps://orcid.org/0000-0001-8407-5226
Lichterfeld, Yannickgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0001-8755-9920
Frett, Timogerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0002-5572-1177
Jordan, Jensgerman aerospace centre (dlr), institute of aerospace medicine, cologne, germanyhttps://orcid.org/0000-0003-4518-0706
Hemmersbach, Ruthgerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0001-5308-6715
Date:7 March 2019
Refereed publication:Yes
Open Access:No
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Status:Published
Keywords:Neuronal regeneration, Altered gravity, Hypergravity, Hypogravity
Event Title:Annual Meeting of the Bonn Forum Biomedizin (BFB)
Event Location:Hennef / Bonn
Event Type:national Conference
Event Dates:7. - 8. März 2019
Organizer:Bonn Forum Biomedizin (BFB)
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):Vorhaben: NeuroTox (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Gravitational Biology
Institute of Aerospace Medicine > Leitungsbereich ME
Deposited By: Duwe, Helmut
Deposited On:30 Apr 2020 09:40
Last Modified:30 Apr 2020 09:40

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