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Molecular response of Deinococcus radiodurans to simulated microgravity explored by proteometabolomic approach

Ott, Emanuel and Fuchs, Felix M. and Moeller, Ralf and Hemmersbach, Ruth and Kawaguchi, Yuko and Yamagishi, Akihiko and Weckwerth, Wolfram and Milojevic, Tetyana (2019) Molecular response of Deinococcus radiodurans to simulated microgravity explored by proteometabolomic approach. Scientific Reports, 9 (1), p. 18462. Nature Publishing Group. doi: 10.1038/s41598-019-54742-6. ISSN 2045-2322.

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Official URL: http://dx.doi.org/10.1038/s41598-019-54742-6

Abstract

Regarding future space exploration missions and long-term exposure experiments, a detailed investigation of all factors present in the outer space environment and their effects on organisms of all life kingdoms is advantageous. Influenced by the multiple factors of outer space, the extremophilic bacterium Deinococcus radiodurans has been long-termly exposed outside the international Space Station in frames of the tanpopo orbital mission. the study presented here aims to elucidate molecular key components in D. radiodurans, which are responsible for recognition and adaptation to simulated microgravity. D. radiodurans cultures were grown for two days on plates in a fast-rotating 2-D clinostat to minimize sedimentation, thus simulating reduced gravity conditions. Subsequently, metabolites and proteins were extracted and measured with mass spectrometry-based techniques. our results emphasize the importance of certain signal transducer proteins, which showed higher abundances in cells grown under reduced gravity. these proteins activate a cellular signal cascade, which leads to differences in gene expressions. Proteins involved in stress response, repair mechanisms and proteins connected to the extracellular milieu and the cell envelope showed an increased abundance under simulated microgravity. focusing on the expression of these proteins might present a strategy of cells to adapt to microgravity conditions.

Item URL in elib:https://elib.dlr.de/134232/
Document Type:Article
Title:Molecular response of Deinococcus radiodurans to simulated microgravity explored by proteometabolomic approach
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iD
Ott, EmanuelExtremophiles/Space Biochemistry Group, Department of Biophysical Chemistry, University of Vienna, Vienna, AustriaUNSPECIFIED
Fuchs, Felix M.Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; felix.fuchs (at) dlr.dehttps://orcid.org/0000-0001-5669-5655
Moeller, RalfRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; ralf.moeller (at) dlr.dehttps://orcid.org/0000-0002-2371-0676
Hemmersbach, RuthGerman Aerospace Center (DLR e.V.), Institute of Aerospace Medicine, Gravitational Biology, Cologne (Köln), Germanyhttps://orcid.org/0000-0001-5308-6715
Kawaguchi, YukoPlanetary Exploration Research Centre (PERC), Chiba Institute of Technology (CIT), Chiba, JapanUNSPECIFIED
Yamagishi, AkihikoDepartment of Life Science and Technology, Tokyo Institute of Technology, Nagatsuta, Yokohama, JapanUNSPECIFIED
Weckwerth, WolframDepartment of Ecogenomics and Systems Biology, University of Vienna, Vienna, Austria and Vienna Metabolomics Centre (VIME), University of Vienna, Vienna, AustriaUNSPECIFIED
Milojevic, TetyanaExtremophiles/Space Biochemistry Group, Department of Biophysical Chemistry, University of Vienna, Vienna, AustriaUNSPECIFIED
Date:5 December 2019
Journal or Publication Title:Scientific Reports
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:9
DOI :10.1038/s41598-019-54742-6
Page Range:p. 18462
Publisher:Nature Publishing Group
ISSN:2045-2322
Status:Published
Keywords:Molecular Response, Deinococcus radiodurans, simulated microgravity
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Gravitational Biology
Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:03 Mar 2020 12:50
Last Modified:29 Sep 2020 17:17

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