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Reactive Jumps Preserve Skeletal Muscle Structure, Phenotype, and Myofiber Oxidative Capacity in Bed Rest

Blottner, D. and Hastermann, M. and Weber, R. and Lenz, R. and Gambara, G. and Limper, U. and Rittweger, J. and Bosutti, A. and Degens, H. and Salanova, M. (2020) Reactive Jumps Preserve Skeletal Muscle Structure, Phenotype, and Myofiber Oxidative Capacity in Bed Rest. Frontiers in Physiology, 10 (1527). Frontiers Media S.A. DOI: 10.3389/fphys.2019.01527 ISSN 1664-042X

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Abstract

Identification of countermeasures able to prevent disuse-induced muscle wasting is crucial to increase performance of crew members during space flight as well as ameliorate patient’s clinical outcome after long immobilization periods. We report on the outcome of short but high-impact reactive jumps (JUMP) as countermeasure during 60 days of 6◦ head-down tilt (HDT) bed rest on myofiber size, type composition, capillarization, and oxidative capacity in tissue biopsies (pre/post/recovery) from the knee extensor vastus lateralis (VL) and deep calf soleus (SOL) muscle of 22 healthy male participants (Reactive jumps in a sledge, RSL-study 2015–2016, DLR:envihab, Cologne). Bed rest induced a slow-to-fast myofiber shift (type I –>II) with an increased prevalence of hybrid fibers in SOL after bed rest without jumps (control, CTRL, p = 0.016). In SOL, JUMP countermeasure in bed rest prevented both fast and slow myofiber cross-sectional area (CSA) decrements (p = 0.005) in CTRL group. In VL, bed rest only induced capillary rarefaction, as reflected by the decrease in local capillaryto-fiber ratio (LCFR) for both type II (pre vs. post/R C 10, p = 0.028/0.028) and type I myofibers (pre vs. R C 10, p = 0.012), which was not seen in the JUMP group. VO2maxFiber (pL × mm−1 × min−1) calculated from succinate dehydrogenase (SDH)- stained cryosections (OD660 nm) showed no significant differences between groups. High-impact jump training in bed rest did not prevent disuse-induced myofiber atrophy in VL, mitigated phenotype transition (type I – >II) in SOL, and attenuated capillary rarefaction in the prime knee extensor VL however with little impact on oxidative capacity changes

Item URL in elib:https://elib.dlr.de/134103/
Document Type:Article
Title:Reactive Jumps Preserve Skeletal Muscle Structure, Phenotype, and Myofiber Oxidative Capacity in Bed Rest
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iD
Blottner, D.Charite - University Medicine BerlinUNSPECIFIED
Hastermann, M.Charite - University Medicine BerlinUNSPECIFIED
Weber, R.Charite - University Medicine BerlinUNSPECIFIED
Lenz, R.German Sports UniversityUNSPECIFIED
Gambara, G.Charité Comprehensive Cancer Center, BerlinUNSPECIFIED
Limper, U.Ulrich.Limper (at) dlr.dehttps://orcid.org/0000-0001-9927-4180
Rittweger, J.Joern.Rittweger (at) dlr.dehttps://orcid.org/0000-0002-2223-8963
Bosutti, A.Department of Clinical, Technological and Morphological Sciences, Division of Internal Medicine, University Triest, ItalienUNSPECIFIED
Degens, H.School of Healthcare Science, Manchester Metropolitan University, Manchester, UKUNSPECIFIED
Salanova, M.Charite - University Medicine BerlinUNSPECIFIED
Date:15 January 2020
Journal or Publication Title:Frontiers in Physiology
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:10
DOI :10.3389/fphys.2019.01527
Publisher:Frontiers Media S.A
ISSN:1664-042X
Status:Published
Keywords:bed rest, disuse, muscle atrophy, capillarization, oxidative capacity, countermeasure
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Systemphysiologie
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Muscle and Bone Metabolism
Deposited By: Becker, Christine
Deposited On:19 Feb 2020 14:44
Last Modified:19 Feb 2020 14:44

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