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Short-Term Microgravity Influences Cell Adhesion in Human Breast Cancer Cells

Nassef, Mohamed Z. and Kopp, Sascha and Melnik, Daniela and Corydon, Thoma J. and Sahana, Jayashree and Krüger, Marcus and Wehland, Markus and Bauer, Thomas J. and Liemersdorf, Christian and Hemmersbach, Ruth and Infanger, Manfred and Grimm, Daniela (2019) Short-Term Microgravity Influences Cell Adhesion in Human Breast Cancer Cells. International Journal of Molecular Sciences, 20 (22), p. 5730. Multidisciplinary Digital Publishing Institute (MDPI). doi: 10.3390/ijms20225730. ISSN 1661-6596.

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Official URL: https://www.mdpi.com/1422-0067/20/22/5730

Abstract

With the commercialization of spaceflight and the exploration of space, it is important to understand the changes occurring in human cells exposed to real microgravity (r-µg) conditions. We examined the influence of r-µg, simulated microgravity (s-µg, incubator random positioning machine (iRPM)), hypergravity (hyper-g), and vibration (VIB) on triple-negative breast cancer (TNBC) cells (MDA-MB-231 cell line) with the aim to study early changes in the gene expression of factors associated with cell adhesion, apoptosis, nuclear factor “kappa-light-chain-enhancer” of activated B-cells (NF-kappaB) and mitogen-activated protein kinase (MAPK) signaling. We had the opportunity to attend a parabolic flight (PF) mission and to study changes in RNA transcription in the MDA-MB cells exposed to PF maneuvers (29th Deutsches Zentrum für Luft- und Raumfahrt (DLR) PF campaign). PF maneuvers induced an early up-regulation of ICAM1, CD44 and ERK1 mRNAs after the first parabola (P1) and a delayed upregulation of NFKB1, NFKBIA, NFKBIB, and FAK1 after the last parabola (P31). ICAM-1, VCAM-1 and CD44 protein levels were elevated, whereas the NF-κB subunit p-65 and annexin-A2 protein levels were reduced after the 31st parabola (P31). The PRKCA, RAF1, BAX mRNA were not changed and cleaved caspase-3 was not detectable in MDA-MB-231 cells exposed to PF maneuvers. Hyper-g-exposure of the cells elevated the expression of CD44 and NFKBIA mRNAs, iRPM-exposure downregulated ANXA2 and BAX, whereas VIB did not affect the TNBC cells. The early changes in ICAM-1 and VCAM-1 and the rapid decrease in the NF-kappaB subunit p-65 might be considered as fast-reacting, gravity-regulated and cell-protective mechanisms of TNBC cells exposed to altered gravity conditions. This data suggest a key role for the detected gravity-signaling elements in three-dimensional growth and metastasis.

Item URL in elib:https://elib.dlr.de/133668/
Document Type:Article
Title:Short-Term Microgravity Influences Cell Adhesion in Human Breast Cancer Cells
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iDORCID Put Code
Nassef, Mohamed Z.Clinic for Plastic, Aesthetic and Hand Surgery, Otto von Guericke University Magdeburg, Magdeburg, GermanyUNSPECIFIEDUNSPECIFIED
Kopp, SaschaOtto von Guericke University, Clinic for Plastic, Aesthetic and Hand Surgery, Magdeburg, Germanyhttps://orcid.org/0000-0001-5829-6455UNSPECIFIED
Melnik, DanielaClinic for Plastic, Aesthetic and Hand Surgery, Otto von Guericke University Magdeburg, Magdeburg, GermanyUNSPECIFIEDUNSPECIFIED
Corydon, Thoma J.Aarhus University, Department of Biomedicine, Aarhus, Denmarkhttps://orcid.org/0000-0003-3588-6350UNSPECIFIED
Sahana, JayashreeAarhus University, Institute of Biomedicine, Aarhus, Denmarkhttps://orcid.org/0000-0002-6606-6315UNSPECIFIED
Krüger, MarcusOtto von Guericke University, Clinic for Plastic, Aesthetic and Hand Surgery, Magdeburg, Germanyhttps://orcid.org/0000-0003-1120-0246UNSPECIFIED
Wehland, MarkusClinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke University Magdeburg, Magdeburg, GermanyUNSPECIFIEDUNSPECIFIED
Bauer, Thomas J.Clinic for Plastic, Aesthetic and Hand Surgery, Otto von Guericke University Magdeburg, Magdeburg, GermanyUNSPECIFIEDUNSPECIFIED
Liemersdorf, Christiangerman aerospace centre (dlr), institute of aerospace medicine, gravitational biology, cologne, germanyhttps://orcid.org/0000-0001-8407-5226UNSPECIFIED
Hemmersbach, RuthGerman Aerospace Center (DLR), Institute of Aerospace Medicine, Gravitational Biology, Cologne, Germanyhttps://orcid.org/0000-0001-5308-6715UNSPECIFIED
Infanger, ManfredClinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke-University Magdeburg, Magdeburg, GermanyUNSPECIFIEDUNSPECIFIED
Grimm, DanielaOtto von Guericke University, Clinic for Plastic, Aesthetic and Hand Surgery, Magdeburg, Germany and Aarhus University, Department of Biomedicine, Aarhus, Denmarkhttps://orcid.org/0000-0002-5083-2812UNSPECIFIED
Date:15 November 2019
Journal or Publication Title:International Journal of Molecular Sciences
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:20
DOI:10.3390/ijms20225730
Page Range:p. 5730
Publisher:Multidisciplinary Digital Publishing Institute (MDPI)
ISSN:1661-6596
Status:Published
Keywords:Breast cancer cells, Microgravity, Hypergravity, Cell adhesion, Apoptosis; NF-kappaB
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Exp.-vorbereitung (old), R - Vorhaben Biowissenschaftliche Nutzerunterstützung (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Gravitational Biology
Deposited By: Duwe, Helmut
Deposited On:21 Jan 2020 09:33
Last Modified:30 Sep 2020 16:09

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