Nassef, Mohamed Z. and Kopp, Sascha and Melnik, Daniela and Corydon, Thoma J. and Sahana, Jayashree and Krüger, Marcus and Wehland, Markus and Bauer, Thomas J. and Liemersdorf, Christian and Hemmersbach, Ruth and Infanger, Manfred and Grimm, Daniela (2019) Short-Term Microgravity Influences Cell Adhesion in Human Breast Cancer Cells. International Journal of Molecular Sciences, 20 (22), p. 5730. Multidisciplinary Digital Publishing Institute (MDPI). doi: 10.3390/ijms20225730. ISSN 1661-6596.
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Official URL: https://www.mdpi.com/1422-0067/20/22/5730
Abstract
With the commercialization of spaceflight and the exploration of space, it is important to understand the changes occurring in human cells exposed to real microgravity (r-µg) conditions. We examined the influence of r-µg, simulated microgravity (s-µg, incubator random positioning machine (iRPM)), hypergravity (hyper-g), and vibration (VIB) on triple-negative breast cancer (TNBC) cells (MDA-MB-231 cell line) with the aim to study early changes in the gene expression of factors associated with cell adhesion, apoptosis, nuclear factor “kappa-light-chain-enhancer” of activated B-cells (NF-kappaB) and mitogen-activated protein kinase (MAPK) signaling. We had the opportunity to attend a parabolic flight (PF) mission and to study changes in RNA transcription in the MDA-MB cells exposed to PF maneuvers (29th Deutsches Zentrum für Luft- und Raumfahrt (DLR) PF campaign). PF maneuvers induced an early up-regulation of ICAM1, CD44 and ERK1 mRNAs after the first parabola (P1) and a delayed upregulation of NFKB1, NFKBIA, NFKBIB, and FAK1 after the last parabola (P31). ICAM-1, VCAM-1 and CD44 protein levels were elevated, whereas the NF-κB subunit p-65 and annexin-A2 protein levels were reduced after the 31st parabola (P31). The PRKCA, RAF1, BAX mRNA were not changed and cleaved caspase-3 was not detectable in MDA-MB-231 cells exposed to PF maneuvers. Hyper-g-exposure of the cells elevated the expression of CD44 and NFKBIA mRNAs, iRPM-exposure downregulated ANXA2 and BAX, whereas VIB did not affect the TNBC cells. The early changes in ICAM-1 and VCAM-1 and the rapid decrease in the NF-kappaB subunit p-65 might be considered as fast-reacting, gravity-regulated and cell-protective mechanisms of TNBC cells exposed to altered gravity conditions. This data suggest a key role for the detected gravity-signaling elements in three-dimensional growth and metastasis.
Item URL in elib: | https://elib.dlr.de/133668/ | ||||||||||||||||||||||||||||||||||||||||||||||||||||
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Document Type: | Article | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Title: | Short-Term Microgravity Influences Cell Adhesion in Human Breast Cancer Cells | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Authors: |
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Date: | 15 November 2019 | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Journal or Publication Title: | International Journal of Molecular Sciences | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Refereed publication: | Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Open Access: | Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Gold Open Access: | Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||
In SCOPUS: | Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||
In ISI Web of Science: | Yes | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Volume: | 20 | ||||||||||||||||||||||||||||||||||||||||||||||||||||
DOI: | 10.3390/ijms20225730 | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Page Range: | p. 5730 | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Publisher: | Multidisciplinary Digital Publishing Institute (MDPI) | ||||||||||||||||||||||||||||||||||||||||||||||||||||
ISSN: | 1661-6596 | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Status: | Published | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Keywords: | Breast cancer cells, Microgravity, Hypergravity, Cell adhesion, Apoptosis; NF-kappaB | ||||||||||||||||||||||||||||||||||||||||||||||||||||
HGF - Research field: | Aeronautics, Space and Transport | ||||||||||||||||||||||||||||||||||||||||||||||||||||
HGF - Program: | Space | ||||||||||||||||||||||||||||||||||||||||||||||||||||
HGF - Program Themes: | Research under Space Conditions | ||||||||||||||||||||||||||||||||||||||||||||||||||||
DLR - Research area: | Raumfahrt | ||||||||||||||||||||||||||||||||||||||||||||||||||||
DLR - Program: | R FR - Research under Space Conditions | ||||||||||||||||||||||||||||||||||||||||||||||||||||
DLR - Research theme (Project): | R - Vorhaben Biowissenschaftliche Exp.-vorbereitung (old), R - Vorhaben Biowissenschaftliche Nutzerunterstützung (old) | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Location: | Köln-Porz | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Institutes and Institutions: | Institute of Aerospace Medicine > Gravitational Biology | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Deposited By: | Duwe, Helmut | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Deposited On: | 21 Jan 2020 09:33 | ||||||||||||||||||||||||||||||||||||||||||||||||||||
Last Modified: | 30 Sep 2020 16:09 |
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