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Beneficial Effects of Low Frequency Vibration on Human Chondrocytes in Vitro

Lützenberg, Roland and Wehland, Markus and Solano, Kendrick and Nassef, Mohamed Z. and Buken, Christoph and Melnik, Daniela and Bauer, Johann and Kopp, Sascha and Krüger, Marcus and Riwaldt, Stefan and Hemmersbach, Ruth and Schulz, Herbert and Infanger, Manfred and Grimm, Daniela (2019) Beneficial Effects of Low Frequency Vibration on Human Chondrocytes in Vitro. Cellular Physiology and Biochemistry, 53 (4), pp. 623-637. S. Karger AG. DOI: 10.33594/000000161 ISSN 1015-8987

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Official URL: https://www.cellphysiolbiochem.com/Articles/000161/

Abstract

Background/Aims: In articular cartilage, chondrocytes are the predominant cell type. A long-term stay in space can lead to bone loss and cartilage breakdown. Due to the poor regenerative capacity of cartilage, this may impair the crewmembers’ mobility and influence mission activities. Beside microgravity other factors such as cosmic radiation and vibration might be important for cartilage degeneration. Vibration at different frequencies showed various effects on cartilage in vivo, but knowledge about its impact on chondrocytes in vitro is sparse. Methods: Human chondrocytes were exposed to a vibration device, simulating the vibration profile occurring during parabolic flights, for 24 h (VIB) and compared to static controls. Phase-contrast microscopy, immunofluorescence, F-actin and TUNEL staining as well as quantitative real-time PCR were performed to examine effects on morphology, cell viability and shape as well as gene expression. The results were compared to earlier studies using semantic analyses. Results: No morphological changes or cytoskeletal alterations were observed in VIB and no apoptotic cells were found. A reorganization and increase in fibronectin were detected in VIB samples by immunofluorescence technique. PXN, VCL, ANXA1, ANXA2, BAX, and BCL2 revealed differential regulations. Conclusion: Long-term VIB did not damage human chondrocytes in vitro. The reduction of ANXA2, and up-regulation of ANXA1, PXN and VCL mRNAs suggest that long-term vibration might even positively influence cultured chondrocytes.

Item URL in elib:https://elib.dlr.de/133406/
Document Type:Article
Title:Beneficial Effects of Low Frequency Vibration on Human Chondrocytes in Vitro
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Lützenberg, Rolandotto-von-guericke-university magdeburg, clinic for plastic, aesthetic and hand surgery, magdeburg, germanyUNSPECIFIED
Wehland, MarkusClinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke University Magdeburg, Magdeburg, GermanyUNSPECIFIED
Solano, Kendrickotto-von-guericke-university magdeburg, clinic for plastic, aesthetic and hand surgery, magdeburg, germanyUNSPECIFIED
Nassef, Mohamed Z.Clinic for Plastic, Aesthetic and Hand Surgery, Otto von Guericke University Magdeburg, Magdeburg, GermanyUNSPECIFIED
Buken, ChristophOtto von Guericke University, Clinic for Plastic, Aesthetic and Hand Surgery, Magdeburg, Germany and Aarhus University, Department of Biomedicine, Aarhus, DenmarkUNSPECIFIED
Melnik, DanielaOtto von Guericke University, Clinic for Plastic, Aesthetic and Hand Surgery, Magdeburg, GermanyUNSPECIFIED
Bauer, Johannmax-planck institute for biochemistry, martinsried, germanyUNSPECIFIED
Kopp, SaschaClinic for Plastic, Aesthetic and Hand Surgery, Otto-von Guericke-University, 39120 Magdeburg, GermanyUNSPECIFIED
Krüger, Marcusotto-von-guericke-university magdeburg, clinic for plastic, aesthetic and hand surgery, magdeburg, germanyUNSPECIFIED
Riwaldt, StefanClinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke-University Magdeburg, Magdeburg, GermanyUNSPECIFIED
Hemmersbach, RuthGerman Aerospace Center (DLR e.V.), Institute of Aerospace Medicine, Gravitational Biology, Cologne (Köln), Germanyhttps://orcid.org/0000-0001-5308-6715
Schulz, HerbertUniversity of Cologne, Cologne Center for Genomics, Cologne, GermanyUNSPECIFIED
Infanger, ManfredClinic for Plastic, Aesthetic and Hand Surgery, Otto-von-Guericke University Magdeburg, Magdeburg, GermanyUNSPECIFIED
Grimm, DanielaClinic for Plastic, Aestetic, and Hand Surgery, Otto von Guericke University Magdeburg, Magdeburg, Germany and Institute of Biomedicine, Pharmacology, Aarhus University, Aarhus, DenmarkUNSPECIFIED
Date:25 September 2019
Journal or Publication Title:Cellular Physiology and Biochemistry
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:No
Volume:53
DOI :10.33594/000000161
Page Range:pp. 623-637
Publisher:S. Karger AG
ISSN:1015-8987
Status:Published
Keywords:Vibration, Chondrocytes, Extracellular matrix, Apoptosis, Focal adhesion, Annexin A2
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Forschung unter Weltraumbedingungen
DLR - Research theme (Project):R - Vorhaben Biowissenschaftliche Exp.-vorbereitung, R - Vorhaben Biowissenschaftliche Nutzerunterstützung
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Gravitational Biology
Deposited By: Duwe, Helmut
Deposited On:16 Jan 2020 12:33
Last Modified:16 Jan 2020 12:33

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