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Single cell whole genome amplification in optofluidic platform and sequencing assessment from the Biology and Mars Experiment (BIOMEX)

Liu, Y. and Jeraldo, P. and Schulze-Makuch, D. and de Vera, Jean Pierre Paul and Cockell, C. and Leya, Thomas and Baque, Mickael and Walther-Antonio, M. (2019) Single cell whole genome amplification in optofluidic platform and sequencing assessment from the Biology and Mars Experiment (BIOMEX). AbSciCon 2019, 24-28 June 2019, Seattle, Washington, USA.

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Abstract

Genome sequencing of single microbial cells in low biomass settings such as in extreme conditions could lead to crucial findings in astrobiological pursuits. Typically, sequencing requires high biomass and averages over genomically heterogeneous populations, concealing the valuable information hidden within very few cells. Single cell whole genome sequencing is capable of identifying microbes with low representation which is often neglected or undetectable in traditional studies. It can also detect rare mutation events in single cells, which allows for mutagenic and detailed evolutionary work. In this work, we use an optofluidic platform that integrates advanced microscopy, laser tweezers and microfluidic technology to isolate single cells of Gleocapsa sp., Sphaerocystis sp. Arctic strain CCCryo 101-99 and Nostoc sp. Antarctic strain CCCryo 231-06 for whole genome amplification and sequencing to identify the genomic variation among the cells exposed to different conditions, including the ground controls (Earth), Mars simulator on Earth, and the simulated Martian condition on the International Space Station. This work presents an unparalleled study of single cell mutagenic events within a population and observation of the raw rate of mutation events that a single cell experiences under these conditions and shed light on microbial responses to space radiation.

Item URL in elib:https://elib.dlr.de/129996/
Document Type:Conference or Workshop Item (Speech)
Title:Single cell whole genome amplification in optofluidic platform and sequencing assessment from the Biology and Mars Experiment (BIOMEX)
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Liu, Y.Mayo ClinicUNSPECIFIED
Jeraldo, P.Mayo ClinicUNSPECIFIED
Schulze-Makuch, D.TU Berlin, GermanyUNSPECIFIED
de Vera, Jean Pierre Pauljean-pierre.devera (at) dlr.deUNSPECIFIED
Cockell, C.UK Centre for Astrobiology, School of Physics and Astronomy, University of Edinburgh, Edinburgh, UK; e-mail: c.s.cockell (at) ed.ac.ukUNSPECIFIED
Leya, ThomasExtremophile Research and Biobank CCCryo, Branch Bioanalytics and Bioprocesses (IZI‐BB), Fraunhofer Institute for Cell Therapy and Immunology, Potsdam, GermanyUNSPECIFIED
Baque, MickaelMickael.Baque (at) dlr.dehttps://orcid.org/0000-0002-6696-6030
Walther-Antonio, M.UNSPECIFIEDUNSPECIFIED
Date:2019
Refereed publication:No
Open Access:Yes
Gold Open Access:No
In SCOPUS:No
In ISI Web of Science:No
Status:Published
Keywords:Genome sequencing, cyanobacteria, Mars habitability, astrobiology, exposure experiments, BIOMEX
Event Title:AbSciCon 2019
Event Location:Seattle, Washington, USA
Event Type:international Conference
Event Dates:24-28 June 2019
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Space Science and Exploration
DLR - Research area:Raumfahrt
DLR - Program:R EW - Erforschung des Weltraums
DLR - Research theme (Project):R - Vorhaben Planetary Evolution and Life
Location: Berlin-Adlershof
Institutes and Institutions:Institute of Planetary Research > Leitungsbereich PF
Deposited By: Baqué, Dr. Mickael
Deposited On:01 Nov 2019 07:51
Last Modified:01 Nov 2019 07:51

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