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Effect of simulated microgravity in the fungus Aspergillus niger

Cortesao, M. und Holland, G. und Laue, M. und Meyer, V. und Moeller, R. (2019) Effect of simulated microgravity in the fungus Aspergillus niger. EANA 2019, 3 – 6 September 2019, Orléans, France.

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Kurzfassung

Fungi are able to colonize indoor-closed habitats such as space stations, in a variety of solid and liquid substrates – e.g. walls, windows, life-support systems, etc. Their growth is usually associated with material degradation and spore formation, which can pose a threat to both astronauts’ health and spacecraft safety, in particular when in long-duration missions [1-3]. This makes monitoring fungal populations a challenge for medical and operation requirements in current and future space missions. Aspergillus niger is one of the predominant fungus detected aboard the Russian Space Station (Mir) as well as the International Space Station (ISS), but it is also known as the model organism for modern biotechnology, producing compounds of interest ranging from citric acid to antibiotics and polymers, among many others [4]. Understanding how the space environment affects fungal growth is not only important to maintain health and safety in spacecraft habitats, but also to assess future opportunities for biotechnology in space. To study how microgravity affects the growth of A. niger, an approach was set to characterize the fungus internal structure under simulated microgravity. For that, A. niger was grown as a colony for 3-5 days in minimum medium at 30°C, in both Earth gravity (1 g) and simulated microgravity (SMG) using a Clinostat [4]. Three different mutant strains were included, to address the effect of melanin and polarized (tip) growth in adapting to the simulated microgravity environment. Colony microstructure was analyzed by newly established scanning electron microscopy (SEM) techniques; changes in colony growth were determined by colony area; sporulation yield was identified by determining the amount of spores produced per colony. Results reveal that simulated microgravity induces changes in colony thickness, colony area and sporulation yield, also suggesting that melanin plays a role in adapting to the low gravity environment. This work marks an important step in the establishment of new electron microscopy methodologies that can be used to study large-dimension samples of filamentous fungi, exposed to different experimental conditions, on Earth or in Space.

elib-URL des Eintrags:https://elib.dlr.de/129875/
Dokumentart:Konferenzbeitrag (Poster)
Titel:Effect of simulated microgravity in the fungus Aspergillus niger
Autoren:
AutorenInstitution oder E-Mail-AdresseAutoren-ORCID-iDORCID Put Code
Cortesao, M.Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; Marta.Cortesao (at) dlr.dehttps://orcid.org/0000-0001-6603-1211NICHT SPEZIFIZIERT
Holland, G.RobertKoch Institute (RKI), Advanced Light and Electron Microscopy, Berlin, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Laue, M.RobertKoch Institute (RKI), Advanced Light and Electron Microscopy, Berlin, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Meyer, V.Department of Applied and Molecular Microbiology, Institute of Biotechnology, Technische Universität Berlin, Berlin, GermanyNICHT SPEZIFIZIERTNICHT SPEZIFIZIERT
Moeller, R.Radiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; ralf.moeller (at) dlr.dehttps://orcid.org/0000-0002-2371-0676NICHT SPEZIFIZIERT
Datum:3 September 2019
Referierte Publikation:Ja
Open Access:Ja
Gold Open Access:Nein
In SCOPUS:Nein
In ISI Web of Science:Nein
Status:veröffentlicht
Stichwörter:simulated microgravity, new electron microscopy methodologies, fungus Aspergillus niger
Veranstaltungstitel:EANA 2019
Veranstaltungsort:Orléans, France
Veranstaltungsart:internationale Konferenz
Veranstaltungsdatum:3 – 6 September 2019
Veranstalter :European Astrobiology Network Association (EANA)
HGF - Forschungsbereich:Luftfahrt, Raumfahrt und Verkehr
HGF - Programm:Raumfahrt
HGF - Programmthema:Forschung unter Weltraumbedingungen
DLR - Schwerpunkt:Raumfahrt
DLR - Forschungsgebiet:R FR - Forschung unter Weltraumbedingungen
DLR - Teilgebiet (Projekt, Vorhaben):R - Vorhaben Strahlenbiologie (alt)
Standort: Köln-Porz
Institute & Einrichtungen:Institut für Luft- und Raumfahrtmedizin > Strahlenbiologie
Hinterlegt von: Kopp, Kerstin
Hinterlegt am:30 Okt 2019 15:02
Letzte Änderung:30 Okt 2019 15:02

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