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Over-Expression of UV-Damage DNA Repair Genes and Ribonucleic Acid Persistence Contribute to the Resilience of Dried Biofilms of the Desert Cyanobacetrium Chroococcidiopsis Exposed to Mars-Like UV Flux and Long-Term Desiccation

Mosca, Claudia and Rothschild, Lynn J. and Napoli, Alessandro and Ferré, Fabrizio and Pietrosanto, Marco and Fagliarone, Claudia and Baqué, Mickael and Rabbow, Elke and Rettberg, Petra and Billi, Daniela (2019) Over-Expression of UV-Damage DNA Repair Genes and Ribonucleic Acid Persistence Contribute to the Resilience of Dried Biofilms of the Desert Cyanobacetrium Chroococcidiopsis Exposed to Mars-Like UV Flux and Long-Term Desiccation. Frontiers in Microbiology, 10. Frontiers Media S.A.. DOI: 10.3389/fmicb.2019.02312 ISSN 1664-302X

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Official URL: http://dx.doi.org/10.3389/fmicb.2019.02312

Abstract

The survival limits of the desert cyanobacterium Chroococcidiopsis were challenged by rewetting dried biofilms and dried biofilms exposed to 1.5 × 10³ kJ/m² of a Mars-like UV, after 7 years of air-dried storage. PCR-stop assays revealed the presence of DNA lesions in dried biofilms and an increased accumulation in dried-UV-irradiated biofilms. Different types and/or amounts of DNA lesions were highlighted by a different expression of uvrA, uvrB, uvrC, phrA, and uvsE genes in dried-rewetted biofilms and dried-UV-irradiatedrewetted biofilms, after rehydration for 30 and 60 min. The up-regulation in dried-rewetted biofilms of uvsE gene encoding an UV damage endonuclease, suggested that UV-damage DNA repair contributed to the repair of desiccation-induced damage. While the phrA gene encoding a photolyase was up-regulated only in dried-UV-irradiated-rewetted biofilms. Nucleotide excision repair genes were over-expressed in dried-rewetted biofilms and dried-UV-irradiated-rewetted biofilms, with uvrC gene showing the highest increase in dried-UV-irradiated-rewetted biofilms. Dried biofilms preserved intact mRNAs (at least of the investigated genes) and 16S ribosomal RNA that the persistence of the ribosome machinery and mRNAs might have played a key role in the early phase recovery. Results have implications for the search of extra-terrestrial life by contributing to the definition of habitability of astrobiologically relevant targets such as Mars or planets orbiting around other stars.

Item URL in elib:https://elib.dlr.de/129600/
Document Type:Article
Title:Over-Expression of UV-Damage DNA Repair Genes and Ribonucleic Acid Persistence Contribute to the Resilience of Dried Biofilms of the Desert Cyanobacetrium Chroococcidiopsis Exposed to Mars-Like UV Flux and Long-Term Desiccation
Authors:
AuthorsInstitution or Email of AuthorsAuthors ORCID iD
Mosca, ClaudiaDepartment of Biology, University of Rome Tor Vergata, Rome, ItalyUNSPECIFIED
Rothschild, Lynn J.Earth Sciences Division, NASA Ames Research Center, Mountain View, CA, United StatesUNSPECIFIED
Napoli, AlessandroDepartment of Biology, University of Rome Tor Vergata, Rome, ItalyUNSPECIFIED
Ferré, FabrizioDepartment of Pharmacy and Biotechnology, University of Bologna Alma Mater, Bologna, ItalyUNSPECIFIED
Pietrosanto, MarcoDepartment of Pharmacy and Biotechnology, University of Bologna Alma Mater, Bologna, ItalyUNSPECIFIED
Fagliarone, ClaudiaDepartment of Pharmacy and Biotechnology, University of Bologna Alma Mater, Bologna, ItalyUNSPECIFIED
Baqué, MickaelMickael.Baque (at) dlr.dehttps://orcid.org/0000-0002-6696-6030
Rabbow, ElkeRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; Elke.Rabbow (at) dlr.dehttps://orcid.org/0000-0002-9301-2021
Rettberg, PetraRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; petra.rettberg (at) dlr.dehttps://orcid.org/0000-0003-4439-2395
Billi, DanielaDepartment of Biology, University of Rome Tor Vergata, Rome, Italy; billi (at) uniroma2.itUNSPECIFIED
Date:11 October 2019
Journal or Publication Title:Frontiers in Microbiology
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:10
DOI :10.3389/fmicb.2019.02312
Publisher:Frontiers Media S.A.
ISSN:1664-302X
Status:Published
Keywords:habitability and astrobiology, anhydrobiosis, desert cyanobacteria, Mars UV simulation, DNA repair
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Space Science and Exploration
DLR - Research area:Raumfahrt
DLR - Program:R EW - Erforschung des Weltraums
DLR - Research theme (Project):R - Vorhaben Planetary Evolution and Life, R - Vorhaben Strahlenbiologie
Location: Berlin-Adlershof , Köln-Porz
Institutes and Institutions:Institute of Planetary Research > Leitungsbereich PF
Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:21 Oct 2019 08:12
Last Modified:21 Oct 2019 08:12

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