Mosca, Claudia and Rothschild, Lynn J. and Napoli, Alessandro and Ferré, Fabrizio and Pietrosanto, Marco and Fagliarone, Claudia and Baqué, Mickael and Rabbow, Elke and Rettberg, Petra and Billi, Daniela (2019) Over-Expression of UV-Damage DNA Repair Genes and Ribonucleic Acid Persistence Contribute to the Resilience of Dried Biofilms of the Desert Cyanobacetrium Chroococcidiopsis Exposed to Mars-Like UV Flux and Long-Term Desiccation. Frontiers in Microbiology, 10, p. 2312. Frontiers Media S.A.. doi: 10.3389/fmicb.2019.02312. ISSN 1664-302X.
PDF
- Published version
833kB |
Official URL: http://dx.doi.org/10.3389/fmicb.2019.02312
Abstract
The survival limits of the desert cyanobacterium Chroococcidiopsis were challenged by rewetting dried biofilms and dried biofilms exposed to 1.5 × 10³ kJ/m² of a Mars-like UV, after 7 years of air-dried storage. PCR-stop assays revealed the presence of DNA lesions in dried biofilms and an increased accumulation in dried-UV-irradiated biofilms. Different types and/or amounts of DNA lesions were highlighted by a different expression of uvrA, uvrB, uvrC, phrA, and uvsE genes in dried-rewetted biofilms and dried-UV-irradiatedrewetted biofilms, after rehydration for 30 and 60 min. The up-regulation in dried-rewetted biofilms of uvsE gene encoding an UV damage endonuclease, suggested that UV-damage DNA repair contributed to the repair of desiccation-induced damage. While the phrA gene encoding a photolyase was up-regulated only in dried-UV-irradiated-rewetted biofilms. Nucleotide excision repair genes were over-expressed in dried-rewetted biofilms and dried-UV-irradiated-rewetted biofilms, with uvrC gene showing the highest increase in dried-UV-irradiated-rewetted biofilms. Dried biofilms preserved intact mRNAs (at least of the investigated genes) and 16S ribosomal RNA that the persistence of the ribosome machinery and mRNAs might have played a key role in the early phase recovery. Results have implications for the search of extra-terrestrial life by contributing to the definition of habitability of astrobiologically relevant targets such as Mars or planets orbiting around other stars.
Item URL in elib: | https://elib.dlr.de/129600/ | ||||||||||||||||||||||||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Document Type: | Article | ||||||||||||||||||||||||||||||||||||||||||||
Title: | Over-Expression of UV-Damage DNA Repair Genes and Ribonucleic Acid Persistence Contribute to the Resilience of Dried Biofilms of the Desert Cyanobacetrium Chroococcidiopsis Exposed to Mars-Like UV Flux and Long-Term Desiccation | ||||||||||||||||||||||||||||||||||||||||||||
Authors: |
| ||||||||||||||||||||||||||||||||||||||||||||
Date: | 11 October 2019 | ||||||||||||||||||||||||||||||||||||||||||||
Journal or Publication Title: | Frontiers in Microbiology | ||||||||||||||||||||||||||||||||||||||||||||
Refereed publication: | Yes | ||||||||||||||||||||||||||||||||||||||||||||
Open Access: | Yes | ||||||||||||||||||||||||||||||||||||||||||||
Gold Open Access: | Yes | ||||||||||||||||||||||||||||||||||||||||||||
In SCOPUS: | Yes | ||||||||||||||||||||||||||||||||||||||||||||
In ISI Web of Science: | Yes | ||||||||||||||||||||||||||||||||||||||||||||
Volume: | 10 | ||||||||||||||||||||||||||||||||||||||||||||
DOI: | 10.3389/fmicb.2019.02312 | ||||||||||||||||||||||||||||||||||||||||||||
Page Range: | p. 2312 | ||||||||||||||||||||||||||||||||||||||||||||
Publisher: | Frontiers Media S.A. | ||||||||||||||||||||||||||||||||||||||||||||
ISSN: | 1664-302X | ||||||||||||||||||||||||||||||||||||||||||||
Status: | Published | ||||||||||||||||||||||||||||||||||||||||||||
Keywords: | habitability and astrobiology, anhydrobiosis, desert cyanobacteria, Mars UV simulation, DNA repair | ||||||||||||||||||||||||||||||||||||||||||||
HGF - Research field: | Aeronautics, Space and Transport | ||||||||||||||||||||||||||||||||||||||||||||
HGF - Program: | Space | ||||||||||||||||||||||||||||||||||||||||||||
HGF - Program Themes: | Space Exploration | ||||||||||||||||||||||||||||||||||||||||||||
DLR - Research area: | Raumfahrt | ||||||||||||||||||||||||||||||||||||||||||||
DLR - Program: | R EW - Space Exploration | ||||||||||||||||||||||||||||||||||||||||||||
DLR - Research theme (Project): | R - Vorhaben Planetary Evolution and Life (old), R - Vorhaben Strahlenbiologie (old) | ||||||||||||||||||||||||||||||||||||||||||||
Location: | Berlin-Adlershof , Köln-Porz | ||||||||||||||||||||||||||||||||||||||||||||
Institutes and Institutions: | Institute of Planetary Research > Leitungsbereich PF Institute of Aerospace Medicine > Radiation Biology | ||||||||||||||||||||||||||||||||||||||||||||
Deposited By: | Kopp, Kerstin | ||||||||||||||||||||||||||||||||||||||||||||
Deposited On: | 21 Oct 2019 08:12 | ||||||||||||||||||||||||||||||||||||||||||||
Last Modified: | 30 Sep 2020 16:07 |
Repository Staff Only: item control page