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Microbial Markers Profile in Anaerobic Mars Analogue Environments Using the LDChip (Life Detector Chip) Antibody Microarray Core of the SOLID (Signs of Life Detector) Platform

García-Descalzo, Laura and Parro, Victorino and García-Villadangos, Miriam and Cockell, Charles S. and Moissl-Eichinger, Christine and Perras, Alex and Rettberg, Petra and Beblo-Vranesevic, Kristina and Bohmeier, Maria and Rabbow, Elke and Westall, Frances and Gaboyer, Frederik and Amils, Ricardo and Malki, Moustafa and Marteinsson, Viggo and Vannier, Pauline and Ehrenfreund, Pascale and Monaghan, Euan and Riedo, Andreas and Cabezas, Patricia and Walter, Nicolas and Gómez, Felipe Gómez (2019) Microbial Markers Profile in Anaerobic Mars Analogue Environments Using the LDChip (Life Detector Chip) Antibody Microarray Core of the SOLID (Signs of Life Detector) Platform. Microorganisms, 7 (9), p. 365. Multidisciplinary Digital Publishing Institute (MDPI). doi: 10.3390/microorganisms7090365. ISSN 2076-2607.

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Official URL: http://dx.doi.org/10.3390/microorganisms7090365

Abstract

One of the main objectives for astrobiology is to unravel and explore the habitability of environments beyond Earth, paying special attention to Mars. If the combined environmental stress factors on Mars are compatible with life or if they were less harsh in the past, to investigate the traces of past or present life is critical to understand its potential habitability. Essential for this research is the characterization of Mars analogue environments on Earth through the development of techniques for biomarker detection in them. Biosensing techniques based on fluorescence sandwich microarray immunoassays (FSMI) have shown to be a powerful tool to detect biosignatures and depict the microbial profiles of different environments. In this study, we described the microbial biomarker profile of five anoxic Mars analogues sites using the Life Detector Chip (LDChip), an antibody microarray for multiple microbial marker detection. Furthermore, we contributed to new targets by developing a new 26-polyclonal antibodies microarray using crude extracts from anaerobic sampling sites, halophilic microorganisms, and anaerobic isolates obtained in the framework of the European Mars Analogues for Space Exploration (MASE) project. The new subset of antibodies was characterized and implemented into a microarray platform (MASE-Chip) for microbial marker searching in salty and anaerobic environments.

Item URL in elib:https://elib.dlr.de/129247/
Document Type:Article
Title:Microbial Markers Profile in Anaerobic Mars Analogue Environments Using the LDChip (Life Detector Chip) Antibody Microarray Core of the SOLID (Signs of Life Detector) Platform
Authors:
AuthorsInstitution or Email of AuthorsAuthor's ORCID iD
García-Descalzo, LauraCentro de Astrobiología (CSIC-INTA), 28850 Madrid, Spain; garciadl (at) cab.inta-csic.esUNSPECIFIED
Parro, VictorinoCentro de Astrobiología (CSIC-INTA), 28850 Madrid, Spain; parrov (at) cab.inta-csic.esUNSPECIFIED
García-Villadangos, MiriamCentro de Astrobiología (CSIC-INTA), 28850 Madrid, Spain; villadangosgm (at) cab.inta-csic.esUNSPECIFIED
Cockell, Charles S.UK Center for Astrobiology, School of Physics and Astronomy, University of Edinburgh, Edinburgh EH9 3FD, UK; c.s.cockell (at) ed.ac.ukhttps://orcid.org/0000-0003-3662-0503
Moissl-Eichinger, ChristineDepartment of Internal Medicine, Medical University of Graz, 8036 Graz, Austria and BioTechMed Graz, 8010 Graz, Austria; christine.moissl-eichinger (at) medunigraz.atUNSPECIFIED
Perras, AlexDepartment of Internal Medicine, Medical University of Graz, 8036 Graz, Austria and Department of Microbiology and Archaea, University of Regensburg, 93040 Regensburg, Germany; akperras (at) gmail.comUNSPECIFIED
Rettberg, PetraRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; petra.rettberg (at) dlr.dehttps://orcid.org/0000-0003-4439-2395
Beblo-Vranesevic, KristinaRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; kristina.beblo (at) dlr.dehttps://orcid.org/0000-0002-4834-7121
Bohmeier, MariaRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; Maria.Bohmeier (at) dlr.deUNSPECIFIED
Rabbow, ElkeRadiation Biology Department, Institute of Aerospace Medicine, German Aerospace Center (DLR), Cologne, Germany; Elke.Rabbow (at) dlr.dehttps://orcid.org/0000-0002-9301-2021
Westall, FrancesCentre de Biophysique Moléculaire, Centre National de la Recherche Scientifique (CNRS), 45100 Orléans, France; frances.westall (at) cnrs-orleans.frUNSPECIFIED
Gaboyer, FrederikCentre de Biophysique Moléculaire, Centre National de la Recherche Scientifique (CNRS), 45100 Orléans, France; frederic.gaboyer (at) cnrs-orleans.frUNSPECIFIED
Amils, RicardoCentro de Biología Molecular Severo Ochoa (CSIC-UAM), 28049 Madrid, Spain and Centro de Biología Molecular Severo Ochoa (CSIC-UAM), 28049 Madrid, Spain; mkasma (at) hotmail.comUNSPECIFIED
Malki, MoustafaCentro de Biología Molecular Severo Ochoa (CSIC-UAM), 28049 Madrid, Spain; mkasma (at) hotmail.comUNSPECIFIED
Marteinsson, ViggoMATIS-Prokaria, 113 Reykjavík, Iceland and Faculty of Food Science and Nutrition, University of Iceland, 101 Reykjavík, Iceland; viggo (at) matis.isUNSPECIFIED
Vannier, PaulineMATIS-Prokaria, 113 Reykjavík, Iceland; pauline.vannier (at) matis.isUNSPECIFIED
Ehrenfreund, PascaleLeiden Observatory, Universiteit Leiden, 2300 Leiden, The Netherlands; p.ehrenfreund (at) chem.leidenuniv.nlUNSPECIFIED
Monaghan, EuanLeiden Observatory, Universiteit Leiden, 2300 Leiden, The Netherlands; monaghan (at) strw.leidenuniv.nlUNSPECIFIED
Riedo, AndreasLeiden Observatory, Universiteit Leiden, 2300 Leiden, The Netherlands; riedo (at) strw.leidenuniv.nlUNSPECIFIED
Cabezas, PatriciaEuropean Science Foundation (ESF), 67080 Strasbourg, France; PCabezas (at) esf.orgUNSPECIFIED
Walter, NicolasEuropean Science Foundation (ESF), 67080 Strasbourg, France; nwalter (at) esf.orgUNSPECIFIED
Gómez, Felipe GómezCentro de Astrobiología (CSIC-INTA), 28850 Madrid, Spain; gomezgf (at) cab.inta-csic.esUNSPECIFIED
Date:18 September 2019
Journal or Publication Title:Microorganisms
Refereed publication:Yes
Open Access:Yes
Gold Open Access:Yes
In SCOPUS:Yes
In ISI Web of Science:Yes
Volume:7
DOI:10.3390/microorganisms7090365
Page Range:p. 365
Publisher:Multidisciplinary Digital Publishing Institute (MDPI)
ISSN:2076-2607
Status:Published
Keywords:anaerobic environments; Mars; analogues; biomarkers; microarray
HGF - Research field:Aeronautics, Space and Transport
HGF - Program:Space
HGF - Program Themes:Research under Space Conditions
DLR - Research area:Raumfahrt
DLR - Program:R FR - Research under Space Conditions
DLR - Research theme (Project):R - Vorhaben Strahlenbiologie (old)
Location: Köln-Porz
Institutes and Institutions:Institute of Aerospace Medicine > Radiation Biology
Deposited By: Kopp, Kerstin
Deposited On:20 Sep 2019 11:35
Last Modified:14 Oct 2020 04:17

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